Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   SM123_RS08250 Genome accession   NZ_CP139419
Coordinates   1703052..1704350 (-) Length   432 a.a.
NCBI ID   WP_129824778.1    Uniprot ID   A0A7X2X5E6
Organism   Streptococcus lingualis strain S5     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1698052..1709350
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SM123_RS08240 (SM123_08240) hpf 1701766..1702308 (-) 543 WP_021154569.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  SM123_RS08245 (SM123_08245) - 1702390..1703055 (-) 666 WP_320909411.1 ComF family protein -
  SM123_RS08250 (SM123_08250) comFA/cflA 1703052..1704350 (-) 1299 WP_129824778.1 DEAD/DEAH box helicase Machinery gene
  SM123_RS08255 (SM123_08255) - 1704406..1705047 (+) 642 WP_320909412.1 YigZ family protein -
  SM123_RS08260 (SM123_08260) cysK 1705170..1706093 (+) 924 WP_003004014.1 cysteine synthase A -
  SM123_RS08265 (SM123_08265) - 1706144..1706509 (-) 366 WP_320909413.1 S1 RNA-binding domain-containing protein -
  SM123_RS08270 (SM123_08270) - 1706511..1707911 (-) 1401 WP_320909414.1 Cof-type HAD-IIB family hydrolase -
  SM123_RS08275 (SM123_08275) - 1707956..1708588 (-) 633 WP_003004009.1 response regulator transcription factor -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49519.56 Da        Isoelectric Point: 9.2527

>NTDB_id=909549 SM123_RS08250 WP_129824778.1 1703052..1704350(-) (comFA/cflA) [Streptococcus lingualis strain S5]
MKIEESYGRLLTESQMTNDLKEIAQELPAIEKQNGRYQCFRCGSLIDQKLWKLSEEVLYCRACIQLGRIRSDQKLYTIAQ
QDFEGQEVLNWKGTLTSYQQEVSEGLIQAVKAGKHALVHAVTGAGKTEMMYQVVATAIKAGKGVCIATPRIDVCIELYGR
MKEDFSCPISLLHGESEPYFRTPLVIATTHQLLKFYQAFDLLIIDEVDAFPYVDNPILYKAAQNAIKKEGNTLYLTATST
DELDKKVKKKEIIRYSLPRRFHGNPLVVPEIKWVPKIREKIEKGRIPYELLQLIKKQRQTHYPLLIFVSEIELGQQFTEN
LKKYFPKETVGFVSSQTTDRLRMVEEFRNRAITMLVSTTILERGVTFPFVDVFVLESNHKLFTKSALVQISGRVGRSKER
PTGKLLFLSDGITREMKKAIKEIKEMNQEAGF

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=909549 SM123_RS08250 WP_129824778.1 1703052..1704350(-) (comFA/cflA) [Streptococcus lingualis strain S5]
ATGAAGATAGAAGAATCTTATGGAAGACTCTTAACGGAGAGTCAAATGACAAATGATTTAAAAGAAATTGCCCAAGAACT
TCCCGCTATAGAAAAACAGAATGGAAGGTACCAATGCTTTCGATGTGGCAGTTTGATTGATCAAAAACTGTGGAAGTTGA
GTGAAGAGGTACTGTATTGTAGAGCCTGTATCCAATTGGGGAGGATCCGGAGTGATCAAAAACTTTATACTATTGCACAG
CAAGACTTTGAAGGACAGGAGGTGTTAAACTGGAAGGGAACCTTGACTTCCTATCAACAAGAGGTATCCGAAGGTCTTAT
CCAAGCAGTTAAGGCAGGAAAACATGCCTTGGTACATGCTGTGACAGGAGCTGGAAAAACGGAAATGATGTATCAGGTTG
TAGCAACAGCGATCAAGGCAGGAAAAGGAGTCTGTATTGCTACCCCGAGAATCGATGTCTGTATCGAACTGTACGGAAGG
ATGAAGGAAGATTTTTCCTGCCCCATCTCTTTACTTCATGGAGAATCGGAACCCTATTTTAGAACACCCTTGGTGATCGC
TACAACCCATCAATTGCTAAAGTTTTATCAGGCCTTTGATCTCCTTATTATAGATGAAGTAGATGCGTTTCCCTATGTGG
ACAATCCAATCTTATATAAAGCAGCTCAGAATGCAATAAAAAAAGAGGGAAATACCCTATATTTAACAGCAACCTCTACA
GATGAGTTAGATAAAAAGGTCAAGAAAAAAGAAATCATTCGTTATAGTCTTCCAAGAAGATTTCATGGGAACCCACTAGT
GGTCCCTGAAATAAAATGGGTGCCGAAAATTAGAGAAAAAATAGAAAAAGGAAGAATTCCTTACGAACTATTGCAACTGA
TCAAGAAACAAAGACAGACTCACTACCCATTGTTAATCTTTGTATCTGAGATAGAATTAGGACAACAATTTACTGAGAAC
TTAAAAAAATACTTTCCAAAAGAAACAGTAGGTTTTGTATCCTCACAGACAACAGACCGCCTACGAATGGTAGAAGAGTT
TAGAAACAGAGCCATAACCATGCTAGTCTCGACAACAATTTTAGAAAGAGGAGTGACTTTTCCATTTGTAGATGTCTTTG
TTTTAGAAAGTAATCACAAATTATTTACTAAAAGTGCCCTCGTACAAATTTCAGGAAGGGTCGGTCGAAGTAAAGAAAGA
CCAACAGGTAAACTACTTTTTCTATCAGATGGTATAACACGAGAAATGAAGAAAGCGATCAAAGAAATAAAGGAAATGAA
TCAGGAGGCTGGATTTTGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(290-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7X2X5E6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

60.277

100

0.604
  comFA/cflA Streptococcus pneumoniae D39

60.277

100

0.604
  comFA/cflA Streptococcus pneumoniae R6

60.277

100

0.604
  comFA/cflA Streptococcus mitis SK321

60.046

100

0.602
  comFA/cflA Streptococcus pneumoniae TIGR4

60.046

100

0.602
  comFA/cflA Streptococcus mitis NCTC 12261

59.815

100

0.6
  comFA Lactococcus lactis subsp. cremoris KW2

50.251

92.13

0.463
  comFA Latilactobacillus sakei subsp. sakei 23K

37.644

100

0.377