Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AABJ74_RS03855 Genome accession   NZ_AP027850
Coordinates   802413..803066 (+) Length   217 a.a.
NCBI ID   WP_338384184.1    Uniprot ID   -
Organism   Escherichia coli strain 2017.18.04CC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 797413..808066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABJ74_RS03850 (VEE64_07450) sslE 797718..802283 (+) 4566 WP_097491262.1 lipoprotein metalloprotease SslE -
  AABJ74_RS03855 (VEE64_07460) pilD 802413..803066 (+) 654 WP_338384184.1 prepilin peptidase Machinery gene
  AABJ74_RS03860 (VEE64_07480) - 803095..803792 (+) 698 WP_103215986.1 IS1-like element IS1A family transposase -
  AABJ74_RS03865 - 803808..803999 (+) 192 Protein_758 A24 family peptidase -
  AABJ74_RS03870 (VEE64_07490) gspS2 804065..804475 (+) 411 WP_001298744.1 type II secretion system pilot lipoprotein GspS-beta -
  AABJ74_RS03875 (VEE64_07500) gspC 804493..805452 (+) 960 WP_000135062.1 type II secretion system protein GspC -
  AABJ74_RS03880 - 805482..805931 (+) 450 Protein_761 hypothetical protein -
  AABJ74_RS03885 gspH 805932..806312 (+) 381 Protein_762 type II secretion system minor pseudopilin GspH -
  AABJ74_RS03890 (VEE64_07520) gspI 806309..806680 (+) 372 WP_000820125.1 type II secretion system minor pseudopilin GspI -
  AABJ74_RS03895 (VEE64_07530) gspJ 806677..807276 (+) 600 WP_001255040.1 type II secretion system minor pseudopilin GspJ -

Sequence

Protein


Download         Length: 217 a.a.        Molecular weight: 24103.58 Da        Isoelectric Point: 7.7270

>NTDB_id=90936 AABJ74_RS03855 WP_338384184.1 802413..803066(+) (pilD) [Escherichia coli strain 2017.18.04CC]
MLFDVFQQYPAAMPVLATVGGLIIGSFLNVVIWRYPIMLRQQMAEFHGEMSSAQSKISLALPRSHCPHCQQTIRVRDNIP
LLSWLMLKGRCRDCQAKISKRYPLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASIIDLDHQWLPDVFTQGVLWT
GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVGDAANLLI

Nucleotide


Download         Length: 654 bp        

>NTDB_id=90936 AABJ74_RS03855 WP_338384184.1 802413..803066(+) (pilD) [Escherichia coli strain 2017.18.04CC]
ATGCTTTTTGATGTTTTTCAGCAATACCCCGCGGCGATGCCCGTCCTGGCAACCGTCGGAGGATTGATCATCGGCAGTTT
TTTGAATGTGGTGATTTGGCGTTACCCCATCATGCTGCGCCAACAAATGGCGGAGTTTCACGGTGAAATGTCGAGTGCGC
AGTCAAAAATAAGCCTTGCGCTGCCGCGTTCGCACTGTCCGCATTGCCAGCAGACTATCCGCGTTCGTGACAATATTCCG
CTGCTCTCCTGGTTGATGCTCAAAGGGCGCTGCCGCGACTGTCAGGCGAAAATCAGCAAGCGTTATCCGCTGGTGGAGTT
ATTGACGGCACTCGCTTTTTTGCTGGCGAGTCTGGTCTGGCCGGAAAGTGGATGGGCGCTGGCGGTGATGATATTATCCG
CCTGGCTGATTGCCGCGAGCATCATCGACCTCGATCACCAATGGCTGCCCGATGTTTTTACTCAGGGCGTATTGTGGACA
GGACTGATTGCGGCATGGGCGCAACAGAGTCCGTTAACGCTACAAGACGCAGTTACCGGCGTCCTGGTGGGGTTTATCAC
TTTTTACTCCCTGCGCTGGATAGCCGGAATAGTTCTGCGTAAAGAAGCATTAGGCATGGGCGATGTAGGTGATGCTGCCA
ACTTACTGATTTAG

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(130-209)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

47.867

97.235

0.465
  pilD Vibrio campbellii strain DS40M4

42.654

97.235

0.415
  pilD Neisseria gonorrhoeae MS11

40.488

94.47

0.382
  pilD Acinetobacter nosocomialis M2

38.389

97.235

0.373
  pilD Acinetobacter baumannii D1279779

37.915

97.235

0.369

Multiple sequence alignment