Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NC3_RS11355 Genome accession   NZ_AP025264
Coordinates   2224756..2225826 (-) Length   356 a.a.
NCBI ID   WP_220482485.1    Uniprot ID   -
Organism   Bacillus altitudinis strain NC3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2219756..2230826
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NC3_RS11305 (NC3_21960) sipW 2219845..2220417 (-) 573 WP_041091749.1 signal peptidase I SipW -
  NC3_RS11310 (NC3_21970) tapA 2220467..2220994 (-) 528 WP_046343966.1 amyloid fiber anchoring/assembly protein TapA -
  NC3_RS11315 (NC3_21980) - 2221247..2221573 (+) 327 WP_039166025.1 DUF3889 domain-containing protein -
  NC3_RS11320 (NC3_21990) - 2221607..2221807 (-) 201 WP_046343970.1 YqzE family protein -
  NC3_RS11325 (NC3_22000) - 2221868..2222251 (-) 384 WP_017357982.1 competence type IV pilus minor pilin ComGG -
  NC3_RS11330 (NC3_22010) comGF 2222248..2222745 (-) 498 WP_017367592.1 competence type IV pilus minor pilin ComGF -
  NC3_RS11335 (NC3_22020) comGE 2222687..2223001 (-) 315 WP_081114339.1 competence type IV pilus minor pilin ComGE -
  NC3_RS11340 (NC3_22030) comGD 2222985..2223431 (-) 447 WP_108611166.1 competence type IV pilus minor pilin ComGD -
  NC3_RS11345 (NC3_22040) comGC 2223424..2223717 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  NC3_RS11350 (NC3_22050) comGB 2223735..2224775 (-) 1041 WP_079920615.1 competence type IV pilus assembly protein ComGB -
  NC3_RS11355 (NC3_22060) comGA 2224756..2225826 (-) 1071 WP_220482485.1 competence type IV pilus ATPase ComGA Machinery gene
  NC3_RS11360 (NC3_22070) - 2226284..2227153 (-) 870 WP_007501231.1 STAS domain-containing protein -
  NC3_RS11370 (NC3_22080) - 2227379..2227759 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  NC3_RS11375 (NC3_22090) - 2227972..2228214 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  NC3_RS11380 (NC3_22100) - 2228263..2228901 (-) 639 WP_017357989.1 MBL fold metallo-hydrolase -
  NC3_RS11385 (NC3_22110) - 2229062..2229235 (+) 174 WP_017357990.1 DUF2759 domain-containing protein -
  NC3_RS11390 (NC3_22120) - 2229459..2229773 (-) 315 WP_008342314.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40348.69 Da        Isoelectric Point: 9.0449

>NTDB_id=90829 NC3_RS11355 WP_220482485.1 2224756..2225826(-) (comGA) [Bacillus altitudinis strain NC3]
MYGIEYLGQELLEEACRMRASDVHIVPREKEASVSFRVDADLIQQRIIDKKSGERLIAHFKFLSSMDIGERRRPQNGSLA
VMLRRGQVFVRLSTLPTVNDESLVIRILPQDHVPKTKYLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKHFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDIIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELACPFCGETCELYCKLNRPVRRTNVFELLYGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEANL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=90829 NC3_RS11355 WP_220482485.1 2224756..2225826(-) (comGA) [Bacillus altitudinis strain NC3]
TTGTATGGTATTGAATATTTGGGGCAAGAGCTGCTAGAAGAGGCGTGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
GAGAGAAAAGGAAGCTTCAGTCTCTTTTCGTGTAGACGCTGACCTCATTCAGCAGCGGATCATTGACAAAAAGAGCGGAG
AGCGGCTCATTGCTCATTTCAAATTTTTATCTTCTATGGATATTGGTGAAAGAAGGAGACCACAAAATGGGTCATTGGCT
GTGATGCTAAGACGCGGTCAAGTGTTTGTTCGGCTCTCTACACTACCGACTGTAAATGATGAAAGTTTAGTGATTCGGAT
TTTACCGCAGGATCATGTGCCGAAAACAAAGTATTTGTCACTGTTCCCGAAGGCTTCAGCGAGATTATTATCGTTTCTGA
ATCATTCGCATGGGCTCATTTTATTTACTGGACCAACAAATTCAGGGAAAACGACAACACTTTATTCATTGATTCAGTTT
GCCAAAAAACATTTTAATCGAAATATTATTACCCTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCTGGTATTACGTATGCGGCAGGTTTACGCGCTATTTTAAGACATGATCCAGATATAATTGTGCTTG
GAGAAATAAGAGATGCGGAAACAGCCAGAACAGCTATTAGAGCAGCTTTGACAGGTCATTTAGTCATGAGCACACTGCAC
GCAAAGAATGCAAAAGGAGCTCTTTATCGAATGCTCGAATTTGGTGTGACGATGAATGAACTCGAACAAACAATGGTTGC
CATTGCTGCTCAGCGGTTAATCGAACTCGCATGTCCATTTTGCGGCGAAACGTGTGAGCTTTATTGCAAACTAAATCGGC
CAGTCAGACGAACAAATGTATTTGAATTGCTTTACGGAAAGGAGCTTGGTGAGTGTATCAAAGAGGCAAAAGGAGAATAC
GCTCATTCGTCATACGAAACACTGCAAAGATTAATTCGGAAAGGAGTGGCACTCGGCTATTTATCAAAAAGCACATATCA
TCGCTGGGTTTATGAAGAAGCGAACCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.607

100

0.646

Multiple sequence alignment