Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   SIS06_RS13540 Genome accession   NZ_CP139188
Coordinates   2560470..2560853 (-) Length   127 a.a.
NCBI ID   WP_283933557.1    Uniprot ID   -
Organism   Bacillus subtilis strain H17-16     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2555470..2565853
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIS06_RS13500 (SIS06_13500) sinI 2556404..2556577 (+) 174 WP_014477323.1 anti-repressor SinI Regulator
  SIS06_RS13505 (SIS06_13505) sinR 2556611..2556946 (+) 336 WP_003226345.1 transcriptional regulator SinR Regulator
  SIS06_RS13510 (SIS06_13510) tasA 2557038..2557823 (-) 786 WP_014477324.1 biofilm matrix protein TasA -
  SIS06_RS13515 (SIS06_13515) sipW 2557888..2558460 (-) 573 WP_003230181.1 signal peptidase I SipW -
  SIS06_RS13520 (SIS06_13520) tapA 2558444..2559205 (-) 762 WP_283933555.1 amyloid fiber anchoring/assembly protein TapA -
  SIS06_RS13525 (SIS06_13525) yqzG 2559477..2559803 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  SIS06_RS13530 (SIS06_13530) spoIITA 2559845..2560024 (-) 180 WP_014480252.1 YqzE family protein -
  SIS06_RS13535 (SIS06_13535) comGG 2560095..2560469 (-) 375 WP_283933556.1 ComG operon protein ComGG Machinery gene
  SIS06_RS13540 (SIS06_13540) comGF 2560470..2560853 (-) 384 WP_283933557.1 ComG operon protein ComGF Machinery gene
  SIS06_RS13545 (SIS06_13545) comGE 2560879..2561226 (-) 348 WP_283933558.1 ComG operon protein 5 Machinery gene
  SIS06_RS13550 (SIS06_13550) comGD 2561210..2561641 (-) 432 WP_283933559.1 comG operon protein ComGD Machinery gene
  SIS06_RS13555 (SIS06_13555) comGC 2561631..2561927 (-) 297 WP_014477332.1 comG operon protein ComGC Machinery gene
  SIS06_RS13560 (SIS06_13560) comGB 2561941..2562978 (-) 1038 WP_283933560.1 comG operon protein ComGB Machinery gene
  SIS06_RS13565 (SIS06_13565) comGA 2562965..2564035 (-) 1071 WP_283933561.1 competence protein ComGA Machinery gene
  SIS06_RS13570 (SIS06_13570) - 2564246..2564443 (-) 198 WP_032726162.1 hypothetical protein -
  SIS06_RS13575 (SIS06_13575) corA 2564445..2565398 (-) 954 WP_015483432.1 magnesium transporter CorA -

Sequence

Protein


Download         Length: 127 a.a.        Molecular weight: 14289.40 Da        Isoelectric Point: 6.2112

>NTDB_id=907635 SIS06_RS13540 WP_283933557.1 2560470..2560853(-) (comGF) [Bacillus subtilis strain H17-16]
MLISGSLAAIFHLFLSRQQEHDGFTQQEWMISIEQMMNECKESQAVKTAEHGSVLICTNLSGQDIRFDIYHSMIRKRVDG
KGHVPILDHIAAMKADFVNGVVLLKIESEDQKVYQTAFPVYSYLGGG

Nucleotide


Download         Length: 384 bp        

>NTDB_id=907635 SIS06_RS13540 WP_283933557.1 2560470..2560853(-) (comGF) [Bacillus subtilis strain H17-16]
TTGCTCATATCAGGATCGTTAGCTGCGATTTTCCATCTGTTTTTGTCTCGACAGCAGGAACATGACGGTTTCACACAGCA
AGAATGGATGATTTCGATAGAACAGATGATGAATGAATGCAAGGAGTCACAGGCAGTTAAGACAGCCGAGCATGGGAGCG
TGTTAATCTGCACCAATCTTTCCGGACAAGACATCCGTTTTGACATTTATCATTCAATGATAAGAAAAAGAGTGGATGGT
AAAGGGCATGTTCCGATTTTAGATCATATTGCTGCCATGAAAGCTGATTTTGTAAATGGTGTTGTTTTGCTGAAAATTGA
GAGTGAGGACCAAAAAGTGTATCAAACTGCTTTTCCAGTCTATTCGTATTTAGGAGGGGGGTGA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(16-114)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Bacillus subtilis subsp. subtilis str. 168

96.85

100

0.969