Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   SIS06_RS01960 Genome accession   NZ_CP139188
Coordinates   368031..368480 (-) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus subtilis strain H17-16     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 363031..373480
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIS06_RS01925 (SIS06_01925) yckA 363314..363994 (-) 681 WP_003234624.1 amino acid ABC transporter permease -
  SIS06_RS01930 (SIS06_01930) yckB 364004..364867 (-) 864 WP_283934040.1 transporter substrate-binding domain-containing protein -
  SIS06_RS01935 (SIS06_01935) - 365029..365217 (+) 189 Protein_338 hypothetical protein -
  SIS06_RS01940 (SIS06_01940) - 365253..365568 (+) 316 Protein_339 RDD family protein -
  SIS06_RS01945 (SIS06_01945) yckD 365650..365982 (+) 333 WP_015252849.1 YckD family protein -
  SIS06_RS01950 (SIS06_01950) bglC 366136..367569 (+) 1434 WP_283934041.1 6-phospho-beta-glucosidase -
  SIS06_RS01955 (SIS06_01955) nin/comJ 367606..368004 (-) 399 WP_032724914.1 DNA-entry nuclease inhibitor Regulator
  SIS06_RS01960 (SIS06_01960) nucA/comI 368031..368480 (-) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  SIS06_RS01965 (SIS06_01965) tlpC 368648..370369 (-) 1722 WP_128746904.1 methyl-accepting chemotaxis protein TlpC -
  SIS06_RS01970 (SIS06_01970) hxlB 370479..371036 (-) 558 WP_021481820.1 6-phospho-3-hexuloisomerase -
  SIS06_RS01975 (SIS06_01975) hxlA 371042..371674 (-) 633 WP_283934042.1 3-hexulose-6-phosphate synthase -
  SIS06_RS01980 (SIS06_01980) hxlR 371906..372268 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=907600 SIS06_RS01960 WP_014475770.1 368031..368480(-) (nucA/comI) [Bacillus subtilis strain H17-16]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=907600 SIS06_RS01960 WP_014475770.1 368031..368480(-) (nucA/comI) [Bacillus subtilis strain H17-16]
GTGAACATCACGACGGACATCATAAAAACAATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCTTCTGACCGTT
ATCCCGAAACGGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987