Detailed information NTG0906834

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: ABNW24_RS20855
• Genome accession: NZ_CP157958
• Coordinates: 4243292..4245490 (-)
• Length: 732 a.a.
• NCBI ID: WP_001301269.1
• Uniprot ID: P17888
• Organism: Escherichia coli strain 800
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4238292..4250490

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABNW24_RS20835 (ABNW24_20835)	hslU	4239096..4240427 (-)	1332	WP_001293341.1	HslU--HslV peptidase ATPase subunit	-
ABNW24_RS20840 (ABNW24_20840)	hslV	4240437..4240967 (-)	531	WP_000208242.1	ATP-dependent protease subunit HslV	-
ABNW24_RS20845 (ABNW24_20845)	ftsN	4241060..4242019 (-)	960	WP_000068828.1	cell division protein FtsN	-
ABNW24_RS20850 (ABNW24_20850)	cytR	4242111..4243136 (-)	1026	WP_000644904.1	DNA-binding transcriptional regulator CytR	Regulator
ABNW24_RS20855 (ABNW24_20855)	priA	4243292..4245490 (-)	2199	WP_001301269.1	primosomal protein N'	Machinery gene
ABNW24_RS20860 (ABNW24_20860)	rpmE	4245693..4245905 (+)	213	WP_000710769.1	50S ribosomal protein L31	-
ABNW24_RS20865 (ABNW24_20865)	yiiX	4245966..4246574 (-)	609	WP_000797353.1	YiiX family permuted papain-like enzyme	-
ABNW24_RS20870 (ABNW24_20870)	metJ	4246758..4247075 (-)	318	WP_000852812.1	met regulon transcriptional regulator MetJ	-
ABNW24_RS20875 (ABNW24_20875)	metB	4247352..4248512 (+)	1161	WP_001295694.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 732 a.a.
• Molecular weight: 81654.91 Da
• Isoelectric Point: 9.0297

>NTDB_id=906834 ABNW24_RS20855 WP_001301269.1 4243292..4245490(-) (priA) [Escherichia coli strain 800]
MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIGIVVSVSDASELPLNELKAVVEVLDSEPVFTHSVWRL
LLWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVLDLKGQKVQ
AGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQHHLRCHHCDSQRPVPRQCPSCG
STHLVPVGLGTEQLEQTLAPLFPGVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERRMMQLPPWTSHVIVR
AEDHNNQHAPLFLQQLRNLILSSPLADEKLWVLGPVPALAPKRGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRK
VKWVLDVDPIEG

Nucleotide

• Length: 2199 bp

>NTDB_id=906834 ABNW24_RS20855 WP_001301269.1 4243292..4245490(-) (priA) [Escherichia coli strain 800]
ATGCCCGTTGCCCACGTTGCCTTGCCCGTTCCGCTTCCTCGTACCTTTGACTATCTGCTGCCAGAAGGCATGACGGTTAA
AGCTGGGTGTCGCGTGCGCGTGCCGTTTGGCAAACAGCAGGAGCGCATCGGGATTGTGGTATCAGTTAGCGATGCCAGCG
AACTGCCGCTCAATGAGCTAAAAGCGGTAGTCGAAGTGCTGGATAGTGAGCCGGTGTTTACTCACTCCGTCTGGCGATTG
CTGCTATGGGCGGCAGATTACTATCATCATCCGATTGGCGATGTGCTGTTTCATGCCTTGCCGATTTTACTACGCCAGGG
GCGGCCTGCGGCGAACGCGCCGATGTGGTACTGGTTTGCCACTGAACAAGGCCAGGCGGTGGATCTGAACAGCCTGAAAC
GCTCCCCCAAGCAACAACAGGCGCTGGCGGCGTTACGGCAAGGCAAAATCTGGCGCGACCAGGTCGCCACGCTCGAATTT
AATGATGCCGCGTTGCAGGCGCTACGCAAAAAAGGTCTGTGTGATTTAGCAAGTGAAACACCAGAGTTTAGCGACTGGCG
AACGAACTATGCCGTTTCTGGTGAGCGGTTGCGATTGAATACCGAACAGGCCACCGCCGTTGGCGCAATTCATAGCGCGG
CAGATACTTTTTCTGCCTGGCTGCTGGCGGGCGTTACCGGTTCCGGTAAAACGGAGGTTTATCTCAGCGTACTGGAAAAC
GTGCTCGCTCAGGGCAAACAGGCGCTGGTGATGGTGCCGGAAATCGGCCTGACACCGCAAACTATCGCCCGTTTTCGTGA
ACGTTTTAATGCCCCCGTGGAAGTTCTGCATTCCGGCCTGAACGACAGCGAGCGTCTTTCGGCGTGGCTGAAAGCGAAAA
ATGGTGAGGCGGCGATTGTGATCGGCACCCGCTCCGCGCTGTTTACGCCGTTTAAAAATCTCGGCGTGATTGTCATTGAT
GAAGAGCACGACAGCTCCTACAAGCAGCAGGAAGGCTGGCGCTATCATGCCCGCGACCTGGCGGTGTATCGTGCGCACAG
CGAGCAAATCCCGATTATTCTTGGCTCCGCAACGCCCGCGCTGGAAACGTTATGCAACGTCCAGCAGAAAAAATACCGCC
TGCTGCGCCTGACCCGTCGGGCAGGGAATGCGCGTCCGGCAATTCAACATGTGCTGGATTTAAAAGGTCAGAAGGTGCAG
GCAGGTCTGGCTCCGGCGTTAATCACTCGTATGCGCCAGCATTTACAGGCTGATAACCAGGTCATTCTCTTTCTTAACCG
CCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGC
TGCATCAGGCGCAGCACCATCTGCGCTGCCACCACTGTGACAGTCAGCGTCCGGTGCCGCGCCAGTGCCCTTCCTGCGGT
TCCACGCACCTGGTCCCCGTGGGGCTGGGCACCGAACAGCTTGAACAGACGCTCGCGCCGTTGTTCCCCGGCGTGCCCAT
TTCTCGTATCGACCGCGATACCACCAGCCGCAAAGGGGCGCTGGAACAGCAACTGGCAGAAGTACATCGCGGCGGCGCGC
GGATTTTGATTGGTACACAAATGCTGGCGAAAGGTCACCATTTCCCGGATGTGACGCTGGTTGCATTACTGGACGTGGAC
GGCGCGCTGTTTTCTGCCGATTTTCGCTCGGCAGAGCGTTTCGCTCAGCTTTACACCCAGGTCGCCGGTCGTGCCGGGCG
TGCGGGTAAACAGGGCGAAGTGGTGCTGCAAACGCACCATCCGGAACATCCTCTGTTGCAAACGTTGCTCTATAAAGGCT
ACGACGCCTTTGCCGAACAGGCGCTGGCTGAGCGGCGAATGATGCAGCTACCGCCGTGGACCAGCCATGTGATTGTGCGT
GCGGAAGATCATAACAATCAGCACGCGCCATTGTTCCTGCAACAACTGCGTAATCTGATCCTCTCCAGCCCACTGGCAGA
CGAGAAACTGTGGGTTCTCGGTCCGGTTCCGGCTCTGGCACCTAAACGTGGCGGTCGCTGGCGCTGGCAGATATTGTTGC
AGCACCCTTCCCGCGTGCGCTTGCAACACATCATTAACGGTACGCTGGCGCTCATCAATACAATACCGGATTCCCGTAAG
GTGAAATGGGTGCTGGATGTTGATCCGATTGAGGGTTAA

Domains

Predicted by InterProScan.

• DEAD/DEAH box helicase (DEAD) (203-362)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (445-471)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (6-101)

• Primosomal protein N C-terminal domain (PriA_C) (632-729)

• Helicase conserved C-terminal domain (Helicase_C) (522-589)

3D structure

• PDB: 2D7E
• PDB: 2D7G
• PDB: 2D7H
• PDB: 2DWL
• PDB: 2DWM
• PDB: 2DWN
• PDB: 6DCR
• PDB: 8FAK

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	44.776	100	0.451