Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   SIC12_RS01325 Genome accession   NZ_CP138985
Coordinates   274271..275983 (-) Length   570 a.a.
NCBI ID   WP_058089902.1    Uniprot ID   A0A142FNH2
Organism   Marinobacter sp. MIT1358     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 269271..280983
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIC12_RS01300 tsaA 269564..270313 (-) 750 WP_058089897.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  SIC12_RS01305 - 270315..271313 (-) 999 WP_227510106.1 hypothetical protein -
  SIC12_RS01310 coaE 271396..272001 (-) 606 WP_058089899.1 dephospho-CoA kinase -
  SIC12_RS01315 pilD 272099..272974 (-) 876 WP_058089900.1 prepilin peptidase Machinery gene
  SIC12_RS01320 pilC 273048..274268 (-) 1221 WP_058089901.1 type II secretion system F family protein Machinery gene
  SIC12_RS01325 pilB 274271..275983 (-) 1713 WP_058089902.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SIC12_RS01330 - 276374..276823 (+) 450 WP_058089903.1 pilin -
  SIC12_RS01335 - 276888..278633 (+) 1746 WP_058089904.1 PglL family O-oligosaccharyltransferase -
  SIC12_RS01340 - 279247..279690 (+) 444 WP_058089985.1 pilin -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62767.85 Da        Isoelectric Point: 4.9523

>NTDB_id=906492 SIC12_RS01325 WP_058089902.1 274271..275983(-) (pilB) [Marinobacter sp. MIT1358]
MASNNRNVTLTGLARRFVDDGLLDEDTAKDAFLQASQNRIPLITYLTQNHLADSSKLAFSAAMEFGVSVLDLDSFLPEMM
PEKVVDEKLVRKHNALPLYKRGNRLFIAVSDPTNIQALDEIKFNTGLSTDAILVDDAKLRTAIDKYLESQDTTMGDLDDA
DLEGVETEGGDQDDDGTVSATEVDDAPIVKYVNKMLLDAIRGGASDIHFEPYEKIYRVRYRTDGILKEISRPSIKLAPKI
SARVKIMAQLDISERRIPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAKLGIDVLGYEEDQKQLYLDALE
QPQGMILVTGPTGSGKTVSLYTGLNILNTPGINISTAEDPAEINLEGINQVNVNTKVGLGFAEALRAFLRQDPDVIMVGE
IRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIATSVSLIIAQRLGRRLCSCKQPADIPKDVLLKEG
FTQEQIDTGFTLYRPKGCDKCSNGYKGRVGIYEVVKITEELASMIMEEASSIKIAKQAQAEGFRNLRQSALLKVMQGVTS
LEEANRVTKD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=906492 SIC12_RS01325 WP_058089902.1 274271..275983(-) (pilB) [Marinobacter sp. MIT1358]
ATGGCGAGCAACAACCGAAACGTGACATTGACAGGCTTAGCCCGTCGATTTGTGGATGACGGGCTTCTGGACGAAGATAC
CGCCAAAGATGCCTTTCTTCAGGCCTCTCAGAACCGCATCCCACTGATAACCTACCTCACCCAGAACCACCTGGCGGACA
GTTCCAAACTCGCGTTTTCTGCTGCCATGGAATTTGGTGTTTCGGTGCTGGACCTTGATTCCTTCCTGCCTGAAATGATG
CCGGAGAAGGTGGTTGACGAGAAATTGGTACGAAAGCATAACGCCCTGCCTCTGTATAAACGAGGCAACCGGCTGTTCAT
CGCAGTATCAGACCCCACAAATATTCAGGCGCTGGATGAGATCAAGTTCAACACCGGGCTTAGCACCGATGCGATTCTGG
TGGACGATGCCAAGCTACGCACAGCCATCGACAAGTATCTCGAATCCCAGGACACCACCATGGGTGACCTGGACGATGCC
GACCTTGAGGGCGTTGAGACCGAGGGTGGTGACCAAGACGACGACGGTACGGTCAGTGCTACCGAAGTAGATGACGCACC
TATTGTTAAGTATGTGAATAAGATGCTGCTTGATGCCATCCGTGGCGGTGCTTCCGATATTCACTTCGAGCCGTATGAAA
AGATTTACCGAGTGCGCTACAGAACCGACGGCATTCTGAAGGAAATCTCCCGCCCGTCCATCAAGCTCGCTCCAAAGATC
TCTGCTCGGGTAAAGATCATGGCGCAGTTGGATATTTCGGAGCGCCGCATTCCTCAGGATGGCCGGATCAAGATGAAGCT
ATCCAAGACCAAGGCCATCGACTTCCGAGTGAACACTTTGCCTACCCTTTGGGGCGAAAAAATTGTTCTTCGGATTCTGG
ACCCTAGCCAGGCCAAGCTGGGCATTGATGTGCTGGGTTACGAGGAAGACCAGAAGCAGCTGTACCTGGACGCACTGGAG
CAGCCTCAGGGCATGATCCTGGTAACTGGCCCTACCGGTTCAGGTAAAACAGTCTCCCTATACACTGGCCTAAACATTCT
CAACACTCCGGGCATCAACATTTCCACGGCCGAAGATCCCGCCGAGATCAACCTGGAGGGTATCAACCAGGTTAACGTAA
ACACCAAAGTGGGCCTCGGCTTCGCTGAAGCCCTTCGGGCCTTCCTGCGGCAAGACCCGGATGTGATCATGGTGGGTGAG
ATCCGGGATCTCGAAACCGCGAACATTGCGATCAAAGCTGCGCAAACCGGGCATTTGGTTCTGTCTACCCTGCACACCAA
CAGCGCGGCCGAAACCCTCACGCGAATGATGAACATGGGGGTGCCTGCATTCAACATTGCCACGTCCGTGAGCCTGATTA
TTGCCCAGCGCCTTGGCCGGCGCCTGTGCAGCTGCAAGCAGCCGGCAGACATCCCGAAAGACGTCCTTTTGAAGGAAGGG
TTCACACAAGAACAAATTGATACAGGCTTCACGTTGTACCGCCCCAAGGGCTGTGATAAATGCTCTAATGGATACAAAGG
CCGCGTCGGTATTTACGAGGTGGTGAAGATTACTGAAGAACTGGCGAGCATGATTATGGAAGAAGCCAGCTCTATAAAAA
TTGCAAAACAAGCGCAGGCAGAAGGTTTCCGCAACCTGAGGCAATCCGCCCTTTTGAAAGTGATGCAAGGGGTGACCAGC
CTTGAGGAAGCCAACCGCGTGACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A142FNH2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.343

100

0.575
  pilB Acinetobacter baylyi ADP1

57.194

98.772

0.565
  pilB Legionella pneumophila strain ERS1305867

53.913

100

0.544
  pilB Vibrio parahaemolyticus RIMD 2210633

52.424

97.719

0.512
  pilB Vibrio campbellii strain DS40M4

51.064

98.947

0.505
  pilB Vibrio cholerae strain A1552

53.757

91.053

0.489
  pilF Neisseria gonorrhoeae MS11

48.763

99.298

0.484
  pilB/pilB1 Synechocystis sp. PCC 6803

40.462

91.053

0.368
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.734

92.281

0.367