Detailed information NTG0904042

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: ABKA22_RS10070
• Genome accession: NZ_CP157287
• Coordinates: 2008827..2011166 (-)
• Length: 779 a.a.
• NCBI ID: WP_003131470.1
• Uniprot ID: Q9CEE5
• Organism: Lactococcus lactis strain BIM B-1834
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2003827..2016166

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABKA22_RS10030 (ABKA22_10030)	-	2004743..2005249 (-)	507	WP_003131479.1	Asp23/Gls24 family envelope stress response protein	-
ABKA22_RS10035 (ABKA22_10035)	-	2005270..2005458 (-)	189	WP_012898445.1	DUF2273 domain-containing protein	-
ABKA22_RS10040 (ABKA22_10040)	amaP	2005469..2006029 (-)	561	WP_010906186.1	alkaline shock response membrane anchor protein AmaP	-
ABKA22_RS10045 (ABKA22_10045)	-	2006057..2006299 (-)	243	WP_003131476.1	GlsB/YeaQ/YmgE family stress response membrane protein	-
ABKA22_RS10050 (ABKA22_10050)	fmt	2006474..2007433 (-)	960	WP_251906059.1	methionyl-tRNA formyltransferase	-
ABKA22_RS10055 (ABKA22_10055)	-	2007430..2007894 (-)	465	WP_003131474.1	VOC family protein	-
ABKA22_RS10060 (ABKA22_10060)	-	2007860..2008381 (-)	522	WP_251906058.1	NUDIX hydrolase	-
ABKA22_RS10065 (ABKA22_10065)	-	2008394..2008768 (-)	375	WP_251906057.1	nuclear transport factor 2 family protein	-
ABKA22_RS10070 (ABKA22_10070)	priA	2008827..2011166 (-)	2340	WP_003131470.1	primosomal protein N'	Machinery gene
ABKA22_RS10075 (ABKA22_10075)	rpoZ	2011305..2011658 (-)	354	WP_010906189.1	DNA-directed RNA polymerase subunit omega	-
ABKA22_RS10080 (ABKA22_10080)	gmk	2011838..2012455 (-)	618	WP_003131468.1	guanylate kinase	-
ABKA22_RS10085 (ABKA22_10085)	rny	2012603..2014198 (-)	1596	WP_003131467.1	ribonuclease Y	-
ABKA22_RS10090 (ABKA22_10090)	-	2014429..2015142 (-)	714	WP_003131466.1	glucosamine-6-phosphate deaminase	-

Sequence

Protein

• Length: 779 a.a.
• Molecular weight: 88929.26 Da
• Isoelectric Point: 6.5580

>NTDB_id=904042 ABKA22_RS10070 WP_003131470.1 2008827..2011166(-) (priA) [Lactococcus lactis strain BIM B-1834]
MKIAKVIVDVPLMQTDKPFSYSLSAEFENLLEVGMRVHVPFGSGNRLVQGIVIGFEDEELEPSVNLKGISELLDVEPVLT
DEQLWLADEMRHSVFSYKISCLKAMLPNLLNSNYDKLLKSTDGTSVKWSQLTEAEKISSAKAVRLGQLSVEYLAKSKENK
KTEKFLSIPDIEKLAEFEIEKRAKKRLELKYFLLRLQEEKIAVRSLNFSSQIIKFFVEHDLVKIEEVEISRTKEKFEDVE
NDSAKILNSEQNAAYEKIIASSDKPFLLEGITGSGKTEVYLQTIDHFLKLGKTAIMLVPEISLTPQITNRFIARFGDKVA
IMHSGLSDGEKYDEWRKIKSGKAQVVVGARSAIFAPVENLGIIIIDEEHETSYKQDSSPRYHARDIALLRAKWHGARLVL
GSATPSLETRARAMRGVYELLLLTKRANEAAILPEIKIIDMRSEISDQSANFSQSLLTKIKEKIDKDEQVILMLNRRGYS
SFVMCRECGFVVDCPNCDISLTLHMDTRTLNCHYCGHVEGIPQTCPNCHSRKIRYYGSGTQKVEEELREIMPEARVLRMD
VDTTRTKNAHEKILEKFGNHEADILLGTQMIAKGLDFPNVTLVGVINADTGLNLPDFRASERSFELLMQVAGRAGRADKK
GEVLIQTFNPHHYAIEMTKAQDYEKFYEMEMRFRRELSYPPYYFTVQVLVSHQDEEVAIQKSYEIAQLLKQNLTEKAKIL
GPTARPIARTHNLYHYQILVKYRFEEELESALNQVLELTQLRDNKNLRVLIDSEPQNFV

Nucleotide

• Length: 2340 bp

>NTDB_id=904042 ABKA22_RS10070 WP_003131470.1 2008827..2011166(-) (priA) [Lactococcus lactis strain BIM B-1834]
ATGAAAATTGCTAAAGTCATCGTTGATGTGCCTCTGATGCAGACGGATAAACCCTTTTCTTACAGCCTTTCAGCTGAATT
TGAAAATTTGCTTGAAGTGGGCATGCGGGTCCATGTTCCTTTTGGAAGTGGAAACCGGCTTGTTCAAGGAATTGTCATTG
GATTTGAAGATGAAGAACTTGAGCCGTCAGTAAATTTGAAAGGAATTTCTGAACTACTCGACGTTGAGCCAGTCCTAACG
GATGAACAGCTTTGGTTGGCAGATGAAATGCGTCATTCAGTATTTTCTTATAAAATTTCATGTCTAAAAGCCATGTTACC
TAATCTTTTGAATTCTAATTATGACAAGCTCCTAAAATCGACTGACGGAACTTCTGTCAAATGGTCACAACTGACAGAAG
CAGAAAAAATCTCGTCAGCAAAGGCTGTGCGTTTGGGTCAGCTGTCAGTAGAATATTTAGCAAAATCTAAAGAAAATAAA
AAAACAGAAAAATTCTTATCTATTCCAGATATCGAAAAACTAGCTGAATTTGAAATTGAAAAGAGAGCTAAAAAACGCTT
AGAGTTGAAATATTTTCTATTAAGACTTCAAGAAGAAAAAATTGCTGTCAGATCATTAAATTTTTCTTCACAAATCATTA
AATTTTTCGTTGAGCATGACCTAGTCAAAATTGAAGAAGTTGAAATTTCGAGGACGAAAGAAAAATTTGAAGATGTGGAA
AATGACAGTGCTAAAATTCTCAACTCTGAACAAAATGCAGCTTACGAAAAAATAATTGCATCTTCTGACAAACCATTTTT
GTTGGAAGGAATTACTGGTTCAGGTAAAACAGAAGTTTACTTACAGACCATTGACCATTTTCTCAAACTCGGTAAAACAG
CAATTATGCTTGTGCCAGAAATTTCTTTGACGCCACAGATTACTAATCGATTTATTGCTCGTTTTGGAGATAAAGTGGCG
ATTATGCATTCTGGTTTATCTGATGGTGAAAAATATGATGAATGGCGAAAAATTAAATCTGGCAAAGCTCAGGTAGTTGT
CGGTGCACGTTCAGCTATTTTTGCACCTGTTGAAAATTTGGGAATTATCATCATTGATGAAGAACATGAAACTTCATATA
AGCAAGATTCAAGTCCCCGTTATCATGCAAGAGATATTGCGCTTTTGCGTGCAAAATGGCATGGTGCAAGACTTGTTTTA
GGTTCTGCAACGCCAAGTTTAGAAACGCGAGCACGTGCCATGCGCGGAGTTTATGAACTTTTATTACTGACAAAAAGGGC
AAATGAAGCAGCAATCCTGCCAGAAATAAAAATCATTGATATGCGCAGTGAAATTTCTGACCAATCTGCTAATTTTTCAC
AATCTTTACTGACAAAAATAAAAGAAAAAATTGACAAAGATGAACAAGTCATTTTGATGTTGAATCGAAGGGGTTATTCT
TCATTTGTCATGTGTCGTGAATGTGGTTTTGTTGTTGATTGTCCCAATTGCGATATTTCCTTGACCCTTCACATGGATAC
CCGAACTTTGAATTGTCATTATTGTGGTCATGTCGAAGGAATTCCGCAAACTTGTCCTAATTGTCATTCACGAAAAATTC
GTTATTACGGTTCAGGAACGCAAAAAGTCGAAGAAGAACTTCGAGAAATCATGCCTGAAGCACGAGTTTTGCGAATGGAT
GTAGATACGACTCGGACAAAAAATGCTCACGAAAAAATTCTTGAAAAGTTTGGTAATCATGAAGCTGATATCCTCTTAGG
AACTCAGATGATTGCCAAAGGTTTAGATTTTCCAAACGTAACCTTAGTGGGTGTCATTAACGCAGATACTGGCCTAAATC
TACCTGATTTTAGAGCGAGTGAGCGTTCCTTTGAATTGTTGATGCAGGTGGCTGGACGAGCTGGACGAGCAGATAAAAAA
GGTGAAGTATTAATTCAAACTTTCAATCCACATCATTATGCTATTGAAATGACCAAGGCTCAAGATTACGAAAAATTTTA
TGAAATGGAAATGAGATTTCGTCGTGAACTTTCCTATCCACCTTATTATTTCACCGTTCAAGTGCTTGTTTCTCATCAAG
ATGAAGAAGTTGCCATTCAAAAATCTTATGAGATCGCTCAATTACTCAAGCAAAATTTGACAGAAAAAGCTAAAATATTA
GGGCCAACCGCCAGACCAATTGCCAGAACACATAACCTTTATCATTATCAAATCTTAGTTAAATATCGTTTTGAAGAAGA
ATTAGAATCAGCTTTAAATCAAGTGCTTGAATTAACCCAACTTCGGGATAACAAAAATTTACGAGTATTGATTGATAGTG
AGCCACAAAATTTTGTATAA

Domains

Predicted by InterProScan.

• Primosomal protein N C-terminal domain (PriA_C) (681-775)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-107)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (494-518)

• Helicase conserved C-terminal domain (Helicase_C) (561-637)

• Type III restriction enzyme, res subunit (ResIII) (247-405)

3D structure

• AlphaFold DB: Q9CEE5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	47.637	100	0.492