Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   Cthiooxydans_RS00590 Genome accession   NZ_AP025193
Coordinates   133087..134472 (-) Length   461 a.a.
NCBI ID   WP_012836620.1    Uniprot ID   A0A5S4SXP0
Organism   Comamonas thiooxydans strain NR4028     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 128087..139472
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Cthiooxydans_RS00565 (Cthiooxydans_01130) - 128874..130001 (-) 1128 WP_087865640.1 glycosyltransferase -
  Cthiooxydans_RS00570 (Cthiooxydans_01140) - 130066..131031 (+) 966 WP_172415328.1 glycosyltransferase family 32 protein -
  Cthiooxydans_RS00575 (Cthiooxydans_01150) - 131077..131286 (-) 210 WP_003059801.1 zinc-finger domain-containing protein -
  Cthiooxydans_RS00580 (Cthiooxydans_01160) - 131431..132369 (-) 939 WP_229227822.1 branched-chain amino acid transaminase -
  Cthiooxydans_RS00585 (Cthiooxydans_01170) - 132451..132906 (-) 456 WP_012836619.1 hypothetical protein -
  Cthiooxydans_RS00590 (Cthiooxydans_01180) radA 133087..134472 (-) 1386 WP_012836620.1 DNA repair protein RadA Machinery gene
  Cthiooxydans_RS00600 (Cthiooxydans_01190) - 134934..136241 (-) 1308 WP_012836621.1 Glu/Leu/Phe/Val family dehydrogenase -
  Cthiooxydans_RS00605 (Cthiooxydans_01200) - 136451..137374 (+) 924 WP_087865128.1 fumarylacetoacetate hydrolase family protein -
  Cthiooxydans_RS00610 (Cthiooxydans_01210) - 137529..138638 (-) 1110 WP_172415262.1 PPK2 family polyphosphate kinase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 48073.39 Da        Isoelectric Point: 7.6670

>NTDB_id=90397 Cthiooxydans_RS00590 WP_012836620.1 133087..134472(-) (radA) [Comamonas thiooxydans strain NR4028]
MAKEKTTFTCNACGGTSPRWLGKCPGCGAWNTLVETVADSASGGKNRLSQPQGYAGLANAQPVTPLSAIEAQDVARTPSG
IEELDRVLGGGVVEGGVVLIGGDPGIGKSTLLLQAMDALHRIGLPTLYVTGEESGAQVALRSRRLGLDNSQVNVLAEIQL
EKILATVEATQPAVVVIDSIQTVYSDQLSSAPGSVAQVRECAAHLTRAAKTTGITIILVGHVTKDGALAGPRVLEHMVDT
VLYFEGDTHSNFRLVRAIKNRFGAVNEIGVFAMTEKGLKGVTNPSAIFLSQHSEPVPGSCVLVTLEGTRPMLVEIQALVD
QGGPAARRLSVGLDRDRLAMLLAVLNRHAGVACADQDVFVNAVGGVRINEPAADLAVMLAITSSLRGKSLPKGFIAFGEV
GLAGEVRPAPRGQERLKEAAKLGFTIAVVPKANAPKKPIAGLTIHAVERVDEAINIVRGIG

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=90397 Cthiooxydans_RS00590 WP_012836620.1 133087..134472(-) (radA) [Comamonas thiooxydans strain NR4028]
ATGGCCAAAGAGAAAACCACTTTCACCTGCAACGCCTGTGGCGGCACCAGCCCACGCTGGCTGGGCAAATGCCCGGGCTG
CGGCGCCTGGAACACCCTGGTCGAGACCGTGGCCGACTCCGCCTCGGGCGGCAAGAACCGCCTGAGCCAGCCCCAGGGCT
ATGCAGGTCTGGCCAATGCCCAGCCCGTCACCCCGTTGTCGGCCATCGAGGCCCAGGATGTGGCGCGCACGCCCAGCGGC
ATCGAGGAGCTGGACCGCGTGCTGGGCGGCGGCGTGGTCGAAGGCGGCGTGGTGCTGATTGGCGGAGATCCTGGAATTGG
TAAGTCCACACTTCTTTTACAGGCCATGGATGCATTGCATCGTATCGGTCTGCCCACGCTCTATGTGACGGGCGAGGAAA
GCGGCGCCCAGGTGGCGCTGCGCTCGCGCCGGCTCGGGCTGGACAACAGTCAGGTCAATGTGCTGGCCGAGATCCAGCTC
GAGAAAATCCTCGCCACCGTGGAGGCCACCCAGCCCGCCGTGGTGGTCATCGACTCGATCCAGACCGTGTATTCGGACCA
GCTCTCCTCGGCTCCGGGCTCCGTGGCCCAGGTGCGCGAATGCGCGGCCCATCTCACGCGTGCGGCCAAGACCACGGGCA
TCACCATCATTCTGGTAGGCCATGTGACCAAGGACGGTGCGCTGGCCGGCCCACGCGTGCTCGAGCACATGGTGGACACG
GTGCTCTACTTCGAAGGCGACACGCACAGCAACTTCCGTCTGGTGCGCGCCATCAAGAACCGCTTTGGCGCCGTCAACGA
GATCGGCGTCTTCGCGATGACGGAAAAAGGCCTCAAGGGCGTGACCAACCCCAGCGCCATCTTTCTGAGTCAGCACAGCG
AACCCGTGCCCGGCAGCTGCGTGCTGGTGACGCTGGAGGGCACGCGCCCCATGCTGGTGGAAATCCAGGCCCTGGTGGAC
CAGGGCGGACCTGCCGCGCGCCGCCTCTCCGTCGGCCTGGACCGCGACCGGCTGGCCATGCTGCTGGCCGTGCTCAACCG
CCATGCGGGCGTGGCCTGCGCCGACCAGGATGTCTTCGTCAATGCCGTGGGTGGCGTGCGCATCAATGAGCCTGCAGCTG
ACCTGGCGGTTATGTTAGCAATCACTTCCAGCCTGCGCGGCAAGTCACTGCCCAAGGGCTTTATCGCGTTTGGCGAGGTC
GGTCTGGCCGGCGAGGTGCGCCCCGCCCCTCGCGGTCAGGAGCGTCTCAAGGAAGCAGCAAAGTTGGGATTCACTATCGC
CGTGGTTCCCAAGGCCAATGCCCCAAAGAAGCCCATCGCCGGCCTGACCATCCATGCCGTGGAGCGCGTTGATGAAGCCA
TCAACATCGTGCGCGGCATTGGCTGA

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(364-455)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(68-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5S4SXP0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.02

99.566

0.488
  radA Streptococcus mitis NCTC 12261

47.598

99.349

0.473
  radA Streptococcus pneumoniae D39

48.438

97.18

0.471
  radA Streptococcus pneumoniae TIGR4

48.438

97.18

0.471
  radA Streptococcus pneumoniae R6

48.438

97.18

0.471
  radA Streptococcus pneumoniae Rx1

48.438

97.18

0.471
  radA Streptococcus mitis SK321

49.425

94.36

0.466

Multiple sequence alignment