Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   SAY89_RS08205 Genome accession   NZ_CP138348
Coordinates   1956037..1958073 (+) Length   678 a.a.
NCBI ID   WP_406584783.1    Uniprot ID   -
Organism   Cyanobacterium aponinum AL20115     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1951037..1963073
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SAY89_RS08180 (SAY89_08180) - 1951042..1952259 (-) 1218 WP_320002242.1 P-aminobenzoate N-oxygenase AurF -
  SAY89_RS08185 (SAY89_08185) - 1952427..1953341 (-) 915 WP_099436709.1 hypothetical protein -
  SAY89_RS08190 (SAY89_08190) - 1953359..1954402 (-) 1044 WP_320002243.1 aromatic ring-hydroxylating dioxygenase subunit alpha -
  SAY89_RS08195 (SAY89_08195) - 1954380..1955141 (-) 762 WP_320002244.1 hypothetical protein -
  SAY89_RS08200 (SAY89_08200) - 1955430..1955702 (-) 273 WP_015218771.1 hypothetical protein -
  SAY89_RS18380 - 1955785..1955925 (-) 141 WP_342596800.1 recombinase family protein -
  SAY89_RS08205 (SAY89_08205) pilB/pilB1 1956037..1958073 (+) 2037 WP_406584783.1 GspE/PulE family protein Machinery gene
  SAY89_RS08210 (SAY89_08210) - 1958514..1959659 (+) 1146 WP_099436712.1 PP2C family protein-serine/threonine phosphatase -
  SAY89_RS08215 (SAY89_08215) rsmD 1960021..1960569 (-) 549 WP_320002245.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  SAY89_RS08220 (SAY89_08220) - 1960592..1961200 (-) 609 WP_320002246.1 precorrin-8X methylmutase -
  SAY89_RS08225 (SAY89_08225) - 1961209..1961985 (-) 777 WP_155084024.1 DUF1868 domain-containing protein -

Sequence

Protein


Download         Length: 678 a.a.        Molecular weight: 75772.76 Da        Isoelectric Point: 4.9578

>NTDB_id=902057 SAY89_RS08205 WP_406584783.1 1956037..1958073(+) (pilB/pilB1) [Cyanobacterium aponinum AL20115]
MTQTPISSASKEKLKTALVSPDYFNPFGNKLIQAGYIDKEDLKRALVQVRNTKQPLVKILENITHKALSPELVRQYKQHQ
LFELKILYGVDCVDPESEEIAADQMTDLIESIMGVQVCRRHKVLPLRRIEGEPPVIVVAMVNPDDLQALDDVKKILRAKG
LDLQRIVITEEDFDKLINRFYEEDKQRQKREEELKEKQKLDQLGDVSDIVDDLDHDLDEAEDENPDGDLTSNDANQAPII
KLVNKILIKALQEGASDIHVEPQETRMRVRLRKDGVLREYFTLPKHITTAVVARFKIMAELDIAERRLPQDGKIRRVFQG
RNVDFRVNCLPSRYGEKVCLRILDNSATQLGLDFLISDEETLAKVRDIASRPFGLILVTGPTGSGKSTTLYSVLAERNDP
GVNISTAEDPIEYALEGITQVQVIREKGMDFASILRAFLRQDPDIILVGETRDAETAKTAIEAALTGHLVLTTLHTNDAA
GAIARLDEMGVEPFMISGALLGVLAQRLMRRVCSECKIAYVPTKEELSRFGLSVSGEESVTFYKANTLSGEALQQAQANG
TVCQKCGGGGYKGRVGVYEFMVMSEELQKLINRGATTDMIKEKAVEEGMVTILAYSLNLVRQGHTTLEEVERVTFTDSGL
EAELKAKRKSSLTCSGCGAELQQEWLDCPYCMTPRFSE

Nucleotide


Download         Length: 2037 bp        

>NTDB_id=902057 SAY89_RS08205 WP_406584783.1 1956037..1958073(+) (pilB/pilB1) [Cyanobacterium aponinum AL20115]
ATGACTCAAACTCCTATTTCTTCTGCTTCTAAAGAAAAACTAAAAACAGCCTTAGTCAGTCCAGATTATTTCAATCCCTT
TGGAAATAAACTAATTCAAGCAGGTTACATTGATAAGGAAGATTTAAAAAGGGCGTTGGTACAAGTGAGAAATACTAAAC
AGCCTTTAGTAAAAATCTTGGAAAATATCACCCACAAGGCTTTGTCTCCTGAGTTAGTGCGACAGTACAAACAACATCAA
CTTTTTGAATTAAAAATTCTTTATGGGGTTGATTGTGTTGACCCAGAGTCAGAAGAAATAGCAGCGGATCAAATGACCGA
TTTAATCGAGTCTATTATGGGAGTTCAGGTTTGTCGTCGTCATAAAGTTTTACCCTTGCGCCGCATTGAGGGTGAACCGC
CTGTAATTGTTGTTGCTATGGTTAATCCTGATGATTTACAGGCGTTGGATGATGTCAAAAAAATATTACGAGCCAAAGGC
CTTGACTTACAAAGAATTGTTATTACTGAAGAAGATTTTGATAAGTTAATTAATAGGTTTTATGAAGAAGATAAGCAAAG
ACAAAAAAGAGAAGAAGAGTTAAAAGAAAAGCAAAAATTAGATCAGTTAGGGGATGTTAGTGATATTGTTGATGATTTGG
ATCATGATTTAGATGAAGCTGAAGACGAAAATCCAGATGGTGATTTGACTTCTAATGATGCTAATCAAGCCCCTATTATC
AAGTTGGTTAATAAAATTCTCATCAAGGCTTTACAAGAAGGGGCATCAGATATTCATGTTGAACCCCAAGAAACCAGAAT
GAGGGTGCGTTTGCGCAAAGATGGGGTTTTAAGGGAATATTTTACTCTTCCTAAACATATTACAACTGCGGTGGTAGCTC
GTTTCAAAATCATGGCTGAGTTGGATATTGCAGAACGCCGTTTACCTCAAGATGGGAAAATTCGTCGGGTGTTTCAGGGA
AGAAATGTGGATTTTCGAGTTAACTGTTTGCCCAGTCGTTACGGTGAAAAGGTCTGTTTAAGGATATTAGATAATTCGGC
TACTCAATTAGGTTTAGACTTTTTAATTTCTGATGAGGAAACATTGGCGAAAGTTAGAGATATTGCCAGTCGTCCTTTTG
GTTTAATTCTGGTTACAGGTCCTACGGGTTCGGGTAAATCCACAACTCTTTATTCTGTTTTGGCGGAGAGAAATGATCCG
GGAGTGAACATAAGTACGGCGGAAGACCCTATTGAGTATGCTTTAGAAGGAATCACTCAGGTGCAAGTAATCCGAGAAAA
AGGTATGGATTTCGCTTCAATTCTTCGGGCTTTTCTCAGACAAGACCCTGATATTATTCTGGTGGGGGAAACACGGGACG
CTGAAACCGCAAAAACTGCGATCGAAGCCGCTTTGACTGGACACTTGGTTTTAACCACTCTACATACTAATGATGCGGCA
GGTGCGATCGCACGTTTGGACGAAATGGGGGTTGAACCCTTTATGATTTCTGGTGCATTGTTAGGGGTTTTGGCACAAAG
ATTAATGCGAAGAGTTTGTAGTGAGTGTAAAATTGCTTATGTTCCTACTAAAGAAGAATTATCCCGTTTTGGTTTATCCG
TATCTGGAGAAGAATCTGTTACCTTTTATAAAGCGAATACTTTGTCAGGAGAAGCCCTACAACAAGCCCAAGCGAATGGC
ACGGTTTGCCAAAAATGTGGTGGCGGTGGTTATAAAGGGCGTGTGGGTGTTTATGAGTTTATGGTTATGAGTGAAGAGTT
GCAAAAACTCATTAACCGAGGAGCAACCACTGACATGATTAAAGAAAAGGCTGTTGAAGAAGGAATGGTAACGATTTTAG
CCTATAGTTTAAATCTAGTTCGTCAAGGTCATACAACCTTAGAAGAAGTTGAAAGGGTAACATTTACTGATTCTGGTTTG
GAAGCAGAATTGAAGGCTAAACGAAAATCATCTTTAACCTGTAGTGGCTGTGGAGCAGAATTACAACAGGAATGGTTAGA
TTGTCCTTATTGTATGACTCCTCGTTTCAGTGAGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(31-185)

Type II/IV secretion system protein (T2SSE)

(245-511)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

69.102

96.903

0.67
  pilF Thermus thermophilus HB27

41.214

92.33

0.381
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.968

92.625

0.37