Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   SBP02_RS02080 Genome accession   NZ_CP137892
Coordinates   431979..433043 (+) Length   354 a.a.
NCBI ID   WP_318644764.1    Uniprot ID   -
Organism   Pseudomonas benzenivorans strain HNIBRBA3361     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 426979..438043
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SBP02_RS02070 (SBP02_02070) - 428012..429280 (+) 1269 WP_318644762.1 malic enzyme-like NAD(P)-binding protein -
  SBP02_RS02075 (SBP02_02075) - 429374..431794 (-) 2421 WP_404824377.1 penicillin-binding protein 1A -
  SBP02_RS02080 (SBP02_02080) pilM 431979..433043 (+) 1065 WP_318644764.1 pilus assembly protein PilM Machinery gene
  SBP02_RS02085 (SBP02_02085) pilN 433043..433615 (+) 573 WP_318644765.1 type 4a pilus biogenesis protein PilN -
  SBP02_RS02090 (SBP02_02090) pilO 433612..434232 (+) 621 WP_318644766.1 type 4a pilus biogenesis protein PilO -
  SBP02_RS02095 (SBP02_02095) pilP 434232..434756 (+) 525 WP_318644767.1 type 4a pilus biogenesis lipoprotein PilP -
  SBP02_RS02100 (SBP02_02100) pilQ 434811..436907 (+) 2097 WP_318646285.1 type IV pilus secretin PilQ Machinery gene
  SBP02_RS02105 (SBP02_02105) aroK 436912..437430 (+) 519 WP_318644768.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37860.11 Da        Isoelectric Point: 4.3692

>NTDB_id=901231 SBP02_RS02080 WP_318644764.1 431979..433043(+) (pilM) [Pseudomonas benzenivorans strain HNIBRBA3361]
MLGLFNKNANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALTRVLSKARSGVKSVAV
AVAGSAVITKTIEMEAGLSDDDLENQLKIEADQYIPYPLEEVAIDFEVQGPSPRNPDRVEVLLAACRKENVEVREAALAL
SGLTAKVVDVEAYALERAYGLLAEQLGGGQEVLTVAVVDIGATMTTLSVLHDGRTIYTREQLFGGKQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTQTLVANP
FADMALGSRVNAGALASDAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=901231 SBP02_RS02080 WP_318644764.1 431979..433043(+) (pilM) [Pseudomonas benzenivorans strain HNIBRBA3361]
GTGCTAGGGCTCTTCAATAAGAATGCGAATACGCTGCTGGGGATCGATATCAGTTCGACCTCGGTCAAGCTCCTCGAGTT
GAGTCGCTCCGGAAGCCGCTACAAGGTAGAGGCTTACGCTGTCGAGCCACTCCCCCCAAATGCTGTGGTCGAGAAGAACA
TCGCCGAGTTGGAAGGCGTTGGTCAGGCGCTTACGCGCGTGTTGAGCAAGGCCAGGAGCGGCGTGAAATCGGTCGCCGTG
GCGGTGGCCGGCTCGGCAGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGACGATGACCTCGAGAATCAGCT
GAAGATCGAGGCTGATCAGTACATCCCCTATCCCCTCGAAGAAGTGGCGATCGACTTCGAAGTGCAAGGGCCTTCGCCGC
GCAACCCCGACCGTGTCGAGGTACTCCTCGCAGCCTGTCGCAAGGAGAACGTCGAGGTTCGAGAGGCCGCGCTGGCCCTG
TCCGGCTTGACCGCCAAGGTGGTCGACGTCGAAGCCTATGCCCTGGAGCGCGCCTACGGCCTGCTTGCGGAGCAACTGGG
CGGCGGGCAAGAGGTGCTGACAGTGGCGGTGGTCGATATCGGTGCGACCATGACGACCCTGAGCGTCCTGCATGATGGGC
GGACCATCTATACGCGCGAGCAACTGTTCGGGGGCAAGCAGCTGACTGAGGAGATCCAGCGGCGTTACGGGCTGTCGGTT
GAGGAAGCTGGGCTCGCCAAGAAGCAGGGAGGCCTTCCAGACGACTATGACAGTGAGGTGTTGCAGCCGTTCAAGGAGGC
TGTCGTACAGCAGGTCTCGCGCTCCTTGCAGTTCTTCTTCGCGGCGGGCCAGTTCAATGATGTCGACTACATCCTCCTGG
CTGGCGGGACGGCCTCCATCCCGGATCTGGATCGGCTGATACAGCAGAAGATCGGTACTCAAACGCTGGTGGCCAATCCG
TTCGCGGACATGGCTCTGGGCAGCAGAGTCAATGCCGGGGCATTGGCCAGCGACGCTCCGTCATTGATGATTGCCTGTGG
GCTGGCGCTGAGGAGTTTTGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

54.237

100

0.542
  pilM Legionella pneumophila strain ERS1305867

45.48

100

0.455