Detailed information NTG0900208

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: R6I04_RS23465
• Genome accession: NZ_CP137714
• Coordinates: 5071136..5072008 (+)
• Length: 290 a.a.
• NCBI ID: WP_003112839.1
• Uniprot ID: P22610
• Organism: Pseudomonas aeruginosa isolate FELIX_MS457
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5066136..5077008

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R6I04_RS23440	nadC	5066477..5067325 (+)	849	WP_003104915.1	carboxylating nicotinate-nucleotide diphosphorylase	-
R6I04_RS23450	pilA	5067527..5067976 (-)	450	WP_003112842.1	type 4a pilus biogenesis protein PilA	Machinery gene
R6I04_RS23455	pilB	5068208..5069908 (+)	1701	WP_003112841.1	type IV-A pilus assembly ATPase PilB	Machinery gene
R6I04_RS23460	pilC	5069912..5071132 (+)	1221	WP_003112840.1	type 4a pilus biogenesis protein PilC	Machinery gene
R6I04_RS23465	pilD	5071136..5072008 (+)	873	WP_003112839.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
R6I04_RS23470	coaE	5072005..5072616 (+)	612	WP_003112838.1	dephospho-CoA kinase	-
R6I04_RS23475	yacG	5072613..5072813 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
R6I04_RS23480	-	5072850..5073059 (-)	210	WP_003094660.1	hypothetical protein	-
R6I04_RS23485	-	5073165..5073854 (-)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
R6I04_RS23490	-	5073851..5074321 (-)	471	WP_003103866.1	hypothetical protein	-
R6I04_RS23495	-	5074318..5074743 (-)	426	WP_003103865.1	GNAT family N-acetyltransferase	-
R6I04_RS23500	-	5074876..5075505 (+)	630	WP_003094668.1	DUF1780 domain-containing protein	-
R6I04_RS23505	-	5075502..5075951 (+)	450	WP_003094670.1	MOSC domain-containing protein	-
R6I04_RS23510	-	5075977..5076147 (+)	171	WP_003094672.1	DUF3094 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31869.92 Da
• Isoelectric Point: 7.7068

>NTDB_id=900208 R6I04_RS23465 WP_003112839.1 5071136..5072008(+) (pilD) [Pseudomonas aeruginosa isolate FELIX_MS457]
MPLLDYLASHPLAFVLCTILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=900208 R6I04_RS23465 WP_003112839.1 5071136..5072008(+) (pilD) [Pseudomonas aeruginosa isolate FELIX_MS457]
ATGCCCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCACCATCCTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCTAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (137-245)

3D structure

• AlphaFold DB: P22610

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.357	96.552	0.534
pilD	Vibrio campbellii strain DS40M4	52.65	97.586	0.514
pilD	Acinetobacter nosocomialis M2	50.53	97.586	0.493
pilD	Acinetobacter baumannii D1279779	50.53	97.586	0.493
pilD	Neisseria gonorrhoeae MS11	50.19	90.69	0.455