Detailed information NTG0899139

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: R6U48_RS01710
• Genome accession: NZ_CP137562
• Coordinates: 350130..351278 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain CPA0087
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 345130..356278

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R6U48_RS01690 (R6U48_01690)	-	345868..347052 (-)	1185	WP_015649763.1	cystathionine gamma-synthase	-
R6U48_RS01695 (R6U48_01695)	-	347049..348422 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
R6U48_RS01700 (R6U48_01700)	-	348665..349069 (-)	405	WP_003084560.1	NINE protein	-
R6U48_RS01705 (R6U48_01705)	czcD	349223..350122 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
R6U48_RS01710 (R6U48_01710)	pilU	350130..351278 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
R6U48_RS01715 (R6U48_01715)	pilT	351456..352490 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
R6U48_RS01720 (R6U48_01720)	-	352705..353397 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
R6U48_RS01725 (R6U48_01725)	proC	353409..354230 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
R6U48_RS01730 (R6U48_01730)	-	354241..354834 (+)	594	WP_003084544.1	YggT family protein	-
R6U48_RS01735 (R6U48_01735)	-	354831..355127 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=899139 R6U48_RS01710 WP_003100959.1 350130..351278(-) (pilU) [Pseudomonas aeruginosa strain CPA0087]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=899139 R6U48_RS01710 WP_003100959.1 350130..351278(-) (pilU) [Pseudomonas aeruginosa strain CPA0087]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGATCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382