Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   R5H20_RS15480 Genome accession   NZ_CP137345
Coordinates   3040724..3041347 (-) Length   207 a.a.
NCBI ID   WP_373926326.1    Uniprot ID   -
Organism   Bacillus sp. KICET-3     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3035724..3046347
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5H20_RS15455 (R5H20_15455) rpsT 3035941..3036207 (+) 267 WP_006637627.1 30S ribosomal protein S20 -
  R5H20_RS15460 (R5H20_15460) holA 3036255..3037295 (-) 1041 WP_006637628.1 DNA polymerase III subunit delta -
  R5H20_RS15465 (R5H20_15465) - 3037528..3037659 (+) 132 WP_006637629.1 YqzM family protein -
  R5H20_RS15470 (R5H20_15470) comEC 3037750..3040080 (-) 2331 WP_006637630.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  R5H20_RS15475 (R5H20_15475) - 3040087..3040656 (-) 570 WP_006637631.1 ComE operon protein 2 -
  R5H20_RS15480 (R5H20_15480) comEA 3040724..3041347 (-) 624 WP_373926326.1 helix-hairpin-helix domain-containing protein Machinery gene
  R5H20_RS15485 (R5H20_15485) comER 3041434..3042255 (+) 822 WP_006637633.1 late competence protein ComER -
  R5H20_RS15490 (R5H20_15490) - 3042323..3043078 (-) 756 WP_006637634.1 class I SAM-dependent DNA methyltransferase -
  R5H20_RS15495 (R5H20_15495) rsfS 3043078..3043431 (-) 354 WP_006637635.1 ribosome silencing factor -
  R5H20_RS15500 (R5H20_15500) yqeK 3043445..3044008 (-) 564 WP_006637636.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  R5H20_RS15505 (R5H20_15505) - 3043998..3044567 (-) 570 WP_006637637.1 nicotinate-nucleotide adenylyltransferase -
  R5H20_RS15510 (R5H20_15510) yhbY 3044586..3044876 (-) 291 WP_006637638.1 ribosome assembly RNA-binding protein YhbY -
  R5H20_RS15515 (R5H20_15515) aroE 3044870..3045706 (-) 837 WP_006637639.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 21720.63 Da        Isoelectric Point: 4.9695

>NTDB_id=897598 R5H20_RS15480 WP_373926326.1 3040724..3041347(-) (comEA) [Bacillus sp. KICET-3]
MNSLKRYKWAAAGVFIAALSVISIMVMPKLHHESAGSDSLPADAASVQAADLAEKKESEEPDRIVVDLKGAVKKPGVYEM
KTGERVHQLLKKAGGTVKNAEGKQINLAAVLQDGMVIYIPFEGEETVQAGTGTAAPSSAEGGTETVNINTASPEELQAIP
GVGPSKAEAIAAYREENGPFQGIEDITNVSGIGEKTFEKIKSSISVK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=897598 R5H20_RS15480 WP_373926326.1 3040724..3041347(-) (comEA) [Bacillus sp. KICET-3]
GTGAATAGTCTGAAACGTTACAAATGGGCTGCGGCGGGAGTCTTTATTGCGGCGCTCTCTGTCATTTCGATCATGGTAAT
GCCAAAACTCCACCATGAATCAGCCGGAAGCGACTCTTTGCCGGCTGATGCCGCATCCGTTCAAGCGGCCGATCTAGCGG
AGAAAAAGGAAAGCGAAGAACCGGACAGAATTGTCGTAGATCTGAAGGGGGCTGTTAAAAAACCGGGCGTTTATGAGATG
AAGACGGGAGAAAGAGTGCACCAATTGCTGAAAAAAGCCGGGGGCACCGTGAAAAACGCAGAGGGAAAACAAATCAATCT
GGCTGCTGTCCTTCAGGACGGCATGGTGATTTATATTCCGTTTGAAGGCGAAGAAACCGTTCAAGCCGGTACAGGAACGG
CAGCACCATCAAGTGCTGAAGGCGGAACGGAAACGGTGAATATCAATACCGCTTCTCCCGAGGAGCTTCAAGCGATCCCC
GGCGTCGGACCTTCAAAAGCGGAGGCGATCGCCGCATACCGTGAAGAGAACGGCCCTTTTCAGGGGATTGAAGACATTAC
AAACGTATCAGGGATTGGCGAAAAAACGTTTGAGAAAATAAAATCGTCAATATCAGTAAAGTAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(144-204)

SLBB domain (SLBB)

(65-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

53.081

100

0.541
  comEA Lactococcus lactis subsp. cremoris KW2

35.514

100

0.367