Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   R4540_RS10955 Genome accession   NZ_CP137135
Coordinates   2412891..2413481 (-) Length   196 a.a.
NCBI ID   WP_000157690.1    Uniprot ID   A0A366AJW1
Organism   Vibrio cholerae V52     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2407891..2418481
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4540_RS10935 (R4540_10930) aroB 2408818..2409903 (-) 1086 WP_000439805.1 3-dehydroquinate synthase -
  R4540_RS10940 (R4540_10935) aroK 2409931..2410455 (-) 525 WP_000818616.1 shikimate kinase AroK -
  R4540_RS10945 (R4540_10940) pilQ 2410642..2412357 (-) 1716 WP_000788426.1 type IV pilus secretin PilQ Machinery gene
  R4540_RS10950 (R4540_10945) pilP 2412383..2412901 (-) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  R4540_RS10955 (R4540_10950) pilO 2412891..2413481 (-) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  R4540_RS10960 (R4540_10955) pilN 2413474..2414058 (-) 585 WP_000902745.1 PilN domain-containing protein Machinery gene
  R4540_RS10965 (R4540_10960) pilM 2414048..2415064 (-) 1017 WP_000520444.1 type IV pilus assembly protein PilM Machinery gene
  R4540_RS10970 (R4540_10965) - 2415207..2417684 (+) 2478 WP_000668739.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22877.27 Da        Isoelectric Point: 4.4169

>NTDB_id=896443 R4540_RS10955 WP_000157690.1 2412891..2413481(-) (pilO) [Vibrio cholerae V52]
MASLQELELDEISEWPLLPQLVVILLIMLLIQGAGYWFYLMPKQDEIALLKQEEETVKATLRIKANKVAVLPQIQAQLDE
LKERYDFLLRQLPVQKELASMLASVNQLGLDSSLTFTRIDWGERESQEFLYRLPLNIELTGSYHDIGDFSQAIAKLPRII
NFDDVDWQRVSQESSTLHFRVRAYTYQFKQEVDDEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=896443 R4540_RS10955 WP_000157690.1 2412891..2413481(-) (pilO) [Vibrio cholerae V52]
ATGGCTAGTCTACAAGAGCTAGAACTGGATGAAATTAGTGAATGGCCGCTGCTGCCTCAGTTGGTGGTGATTCTGCTTAT
CATGCTACTGATCCAAGGTGCTGGTTATTGGTTCTATCTCATGCCCAAACAAGACGAGATTGCGCTGTTAAAGCAGGAAG
AGGAAACCGTCAAGGCGACACTGCGCATCAAAGCCAATAAAGTGGCGGTATTGCCGCAGATCCAAGCTCAGTTGGACGAG
CTCAAAGAGCGTTACGATTTTTTACTGCGCCAGCTACCGGTGCAAAAAGAGTTAGCCAGCATGCTGGCGTCAGTCAACCA
GCTTGGGCTCGATAGTTCGCTTACCTTTACGCGGATTGATTGGGGGGAGCGTGAAAGCCAAGAGTTTCTTTACCGCTTAC
CGCTCAATATCGAACTGACGGGGAGCTATCACGATATTGGCGATTTTTCGCAAGCGATTGCCAAGTTACCGCGCATCATC
AACTTCGACGATGTGGACTGGCAGCGTGTCAGCCAAGAGAGTAGCACGCTGCACTTTCGAGTCAGAGCCTACACCTACCA
ATTTAAGCAGGAGGTGGATGATGAAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(50-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A366AJW1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

100

100

1
  pilO Vibrio campbellii strain DS40M4

71.958

96.429

0.694