Detailed information    

insolico Bioinformatically predicted

Overview

Name   clpE   Type   Regulator
Locus tag   AAHT64_RS12965 Genome accession   NZ_CP155054
Coordinates   2535895..2537994 (+) Length   699 a.a.
NCBI ID   WP_009967104.1    Uniprot ID   O31673
Organism   Bacillus subtilis subsp. subtilis strain R38     
Function   repress competence development (at the early growth phase) (predicted from homology)   
Competence regulation

Genomic Context

Location: 2530895..2542994
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAHT64_RS12935 (AAHT64_12935) ykvN 2530919..2531275 (+) 357 WP_003245808.1 winged helix-turn-helix transcriptional regulator -
  AAHT64_RS12940 (AAHT64_12940) queF 2531834..2532331 (-) 498 WP_003218613.1 preQ(1) synthase -
  AAHT64_RS12945 (AAHT64_12945) queE 2532349..2533080 (-) 732 WP_003232460.1 7-carboxy-7-deazaguanine synthase QueE -
  AAHT64_RS12950 (AAHT64_12950) queD 2533073..2533522 (-) 450 WP_003232462.1 6-carboxytetrahydropterin synthase QueD -
  AAHT64_RS12955 (AAHT64_12955) queC 2533515..2534174 (-) 660 WP_003245417.1 7-cyano-7-deazaguanine synthase QueC -
  AAHT64_RS12960 (AAHT64_12960) - 2534487..2535530 (-) 1044 WP_003244712.1 hypothetical protein -
  AAHT64_RS12965 (AAHT64_12965) clpE 2535895..2537994 (+) 2100 WP_009967104.1 ATP-dependent protease ATP-binding subunit ClpE Regulator
  AAHT64_RS12970 (AAHT64_12970) motA 2538377..2539189 (+) 813 WP_003244739.1 flagellar motor stator protein MotA -
  AAHT64_RS12975 (AAHT64_12975) motB 2539161..2539946 (+) 786 WP_003232473.1 flagellar motor protein MotB -
  AAHT64_RS12980 (AAHT64_12980) mhqR 2539986..2540423 (-) 438 WP_003232475.1 MarR family transcriptional regulator MhqR -
  AAHT64_RS12985 (AAHT64_12985) kinD 2540616..2542136 (+) 1521 WP_010886497.1 sporulation kinase KinD -
  AAHT64_RS12990 (AAHT64_12990) - 2542164..2542595 (-) 432 WP_003245685.1 hypothetical protein -
  AAHT64_RS12995 (AAHT64_12995) spo0E 2542681..2542938 (-) 258 WP_003218598.1 aspartyl-phosphate phosphatase Spo0E -

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 77905.96 Da        Isoelectric Point: 5.0886

>NTDB_id=895547 AAHT64_RS12965 WP_009967104.1 2535895..2537994(+) (clpE) [Bacillus subtilis subsp. subtilis strain R38]
MRCQHCHQNEATIRLNMQINSVHKQMVLCETCYNELTRKPSMSMGPQSFGFPFEQAFQPKEQSAAKQSEKKGLLDELAQN
ITNGAKAGLIDPVIGRDDEVARVIEILNRRNKNNPVLIGEPGVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVA
NTGIRGQFEERMKQLITELKERKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAAL
ERRFQPVMVQEPSIEQAILILQGIKDKYEAYHGVTFSDEAIKACVTLSSRYIQDRHLPDKAIDLLDEAGSKANLLIDELN
DEDAAERLTAIEAEKTKALEEENYELAAKLRDEELALEKKLNSSSAHTAVTVEAEHIQEIVEQKTGIPVGKLQADEQTKM
KELEAKLHERVIGQEAAVQKVAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKTLADELFGTKDAIIRLDMSEYME
KHAVSKIIGSPPGYVGHEEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTDSQGRTVSFKDTVIIMTSN
AGAGEKQTKVGFQSDDSVIEEQTLIDSLSMFFKPEFLNRFDSIIEFRSLEKEHLVKIVSLLLGELEETLAERGISLNVTD
EAKEKIAELGYHPSFGARPLRRTIQEWVEDEMTDLLLDNGEITSFHVILEDDKIKVRAK

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=895547 AAHT64_RS12965 WP_009967104.1 2535895..2537994(+) (clpE) [Bacillus subtilis subsp. subtilis strain R38]
ATGCGTTGTCAACATTGTCATCAAAACGAGGCGACGATTCGCCTTAACATGCAAATAAATTCCGTTCATAAACAGATGGT
TCTTTGTGAAACTTGCTATAACGAACTGACCCGTAAACCTTCAATGAGTATGGGTCCTCAATCTTTCGGATTTCCGTTTG
AACAGGCATTCCAGCCGAAAGAACAGAGCGCAGCAAAACAAAGCGAAAAAAAAGGGTTGCTTGATGAGCTGGCTCAAAAT
ATTACAAACGGTGCAAAAGCCGGTCTCATTGATCCCGTCATCGGCCGTGATGATGAAGTGGCGCGAGTGATCGAAATTCT
AAACCGCCGAAACAAAAACAATCCGGTTCTTATTGGTGAGCCGGGTGTAGGGAAAACTGCCATCGCTGAAGGGCTCGCTT
TAAAAATTGCTGAAGGTGATGTTCCAAACAAACTAAAAAACAAAGAGCTATACTTGCTTGATGTTGCATCTCTTGTTGCA
AACACAGGGATCAGAGGCCAATTTGAGGAGAGAATGAAACAGCTGATCACTGAGCTGAAGGAACGAAAAAATGTCATTCT
GTTCATTGATGAAATTCACCTTCTCGTCGGCGCAGGCTCTGCAGAAGGATCAATGGACGCCGGCAACATTCTCAAACCGG
CCCTAGCCAGAGGCGAACTGCAAGTCATTGGCGCGACAACACTGAAAGAATATCGTCAAATCGAAAAAGATGCCGCGCTG
GAAAGACGTTTTCAGCCTGTCATGGTGCAGGAGCCTTCAATTGAACAGGCTATCCTCATTCTGCAAGGGATTAAAGACAA
ATACGAGGCGTACCATGGCGTAACATTCAGTGATGAAGCAATCAAAGCATGTGTCACTTTATCATCCCGCTACATTCAGG
ACAGACACCTGCCGGATAAAGCAATTGATTTATTAGATGAAGCAGGTTCAAAAGCCAACCTGTTAATTGATGAACTGAAT
GATGAGGATGCCGCTGAACGCTTAACTGCAATTGAAGCCGAAAAAACAAAAGCCCTGGAAGAAGAAAATTACGAACTGGC
GGCAAAACTCCGTGATGAAGAACTCGCATTGGAGAAAAAACTGAACAGCTCCTCCGCTCATACCGCTGTCACTGTGGAAG
CTGAGCACATTCAGGAAATTGTTGAACAAAAAACAGGCATCCCTGTCGGCAAACTGCAGGCAGACGAACAAACGAAAATG
AAAGAACTCGAAGCAAAACTTCATGAACGCGTGATCGGACAAGAAGCCGCTGTTCAAAAAGTGGCAAAAGCGGTAAGACG
AAGCCGCGCCGGATTAAAATCCAAAAACAGACCAGTCGGCTCCTTCCTCTTCGTCGGTCCTACCGGCGTAGGGAAAACAG
AGCTTTCTAAAACACTGGCAGATGAATTATTCGGCACAAAAGACGCTATTATCCGACTCGATATGAGCGAATACATGGAG
AAACACGCCGTATCTAAAATTATCGGTTCACCGCCTGGATATGTCGGCCATGAGGAAGCTGGACAATTAACTGAGAAGGT
GCGCCGCAATCCTTACAGCATTGTGTTGCTGGATGAGATTGAAAAAGCACACCCAGACGTTCAGCATATGTTCCTGCAAA
TTATGGAAGATGGCCGTCTGACAGACAGCCAAGGCAGAACTGTAAGCTTCAAAGACACTGTGATCATCATGACAAGTAAT
GCGGGTGCTGGTGAGAAACAAACGAAAGTCGGTTTCCAATCAGATGACAGTGTCATCGAAGAACAAACATTAATTGATTC
ACTGAGCATGTTCTTTAAACCTGAGTTCCTCAACCGTTTTGACAGCATTATTGAGTTCCGCTCATTGGAAAAAGAACATC
TTGTCAAAATCGTCAGCCTTCTTCTTGGAGAACTTGAAGAAACATTGGCGGAACGGGGCATTAGCTTGAATGTGACAGAT
GAAGCGAAAGAAAAAATCGCTGAGCTGGGCTACCACCCTTCATTCGGTGCACGTCCGCTTAGAAGAACCATCCAAGAATG
GGTTGAGGATGAAATGACCGATCTGCTGCTTGATAATGGCGAGATCACAAGTTTTCACGTGATTTTAGAAGATGATAAAA
TCAAAGTGCGAGCAAAATAA

Domains

Predicted by InterProScan.

AAA domain (Cdc48 subfamily) (AAA_2)

(437-603)

ATPase family associated with various cellular activities (AAA) (AAA)

(116-247)

AAA lid domain (AAA_lid_9)

(253-352)

C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small)

(609-679)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O31673

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpE Streptococcus mutans UA159

56.662

100

0.602
  clpC Lactococcus lactis subsp. cremoris KW2

56.284

100

0.589
  clpE Streptococcus pneumoniae TIGR4

54.924

100

0.567
  clpE Streptococcus pneumoniae Rx1

54.924

100

0.567
  clpE Streptococcus pneumoniae D39

54.924

100

0.567
  clpE Streptococcus pneumoniae R6

54.924

100

0.567
  clpC Bacillus subtilis subsp. subtilis str. 168

54.016

90.844

0.491
  clpC Lactococcus lactis subsp. lactis strain DGCC12653

47.847

89.7

0.429
  clpC Streptococcus thermophilus LMD-9

45.427

93.848

0.426
  clpC Streptococcus pneumoniae D39

46.25

91.559

0.423
  clpC Streptococcus pneumoniae Rx1

46.25

91.559

0.423
  clpC Streptococcus thermophilus LMG 18311

44.97

93.848

0.422
  clpC Streptococcus pneumoniae TIGR4

46.85

88.555

0.415
  clpC Streptococcus mutans UA159

46.216

88.841

0.411
  clpA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

42.079

86.695

0.365