Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA3   Type   Machinery gene
Locus tag   LDO80_RS06745 Genome accession   NZ_AP024933
Coordinates   1248222..1248923 (+) Length   233 a.a.
NCBI ID   WP_224065290.1    Uniprot ID   -
Organism   Thermus thermophilus strain AA3-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1243222..1253923
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LDO80_RS06720 (TthAA37_13330) ilvB 1243658..1245346 (-) 1689 WP_223965550.1 biosynthetic-type acetolactate synthase large subunit -
  LDO80_RS06725 (TthAA37_13340) - 1245502..1246482 (-) 981 WP_018461695.1 YpdA family putative bacillithiol disulfide reductase -
  LDO80_RS06730 (TthAA37_13350) pilA/pilA1 1246648..1247118 (+) 471 WP_143585821.1 GspH/FimT family protein Machinery gene
  LDO80_RS06735 (TthAA37_13360) - 1247174..1247647 (+) 474 WP_223965551.1 GspH/FimT family protein -
  LDO80_RS06740 (TthAA37_13370) pilA/pilA2 1247665..1248225 (+) 561 WP_267461250.1 type IV pilus modification PilV family protein Machinery gene
  LDO80_RS06745 (TthAA37_13380) pilA/pilA3 1248222..1248923 (+) 702 WP_224065290.1 PilW family protein Machinery gene
  LDO80_RS06750 (TthAA37_13390) comZ 1248934..1250610 (+) 1677 WP_223965554.1 pilus assembly PilX N-terminal domain-containing protein Machinery gene
  LDO80_RS13220 - 1250828..1250942 (+) 115 Protein_1323 type IV pilin protein -
  LDO80_RS06760 (TthAA37_13410) - 1251625..1252005 (+) 381 WP_223968480.1 DUF1640 domain-containing protein -
  LDO80_RS06765 (TthAA37_13420) - 1252081..1252623 (+) 543 WP_223968482.1 Uma2 family endonuclease -
  LDO80_RS06770 (TthAA37_13430) - 1252646..1252945 (+) 300 WP_008631315.1 nucleotidyltransferase family protein -
  LDO80_RS06775 (TthAA37_13440) - 1252932..1253294 (+) 363 WP_060384345.1 DUF86 domain-containing protein -
  LDO80_RS06780 (TthAA37_13450) - 1253328..1253885 (-) 558 WP_223970927.1 Uma2 family endonuclease -

Sequence

Protein


Download         Length: 233 a.a.        Molecular weight: 24963.36 Da        Isoelectric Point: 6.2917

>NTDB_id=89533 LDO80_RS06745 WP_224065290.1 1248222..1248923(+) (pilA/pilA3) [Thermus thermophilus strain AA3-7]
MKRGFTLVEVLVAMAILVVVLAVDVRYFASTSELARNTQARSELQDRVRMVMQVVTADLQMAGARYWNSGDQNQAFSLPL
PPLSGSDGGPKDTLTLYYVTSLRDLASACRRVDYGFEGDTLRRSDVSATPTSGSDCTTPSPNYQPLAEGILALDIQYQCS
DGTTRNTPDCGANAYPRSAKVTVAGYSLTPVTNPGPASLTTVTGKTLACPQGRACYALTQEVLMPNLKPLPTP

Nucleotide


Download         Length: 702 bp        

>NTDB_id=89533 LDO80_RS06745 WP_224065290.1 1248222..1248923(+) (pilA/pilA3) [Thermus thermophilus strain AA3-7]
ATGAAGCGCGGCTTCACCCTGGTGGAGGTCCTGGTGGCCATGGCCATCTTGGTGGTGGTCCTGGCCGTGGACGTGCGCTA
CTTCGCAAGCACAAGCGAACTCGCCCGGAACACCCAGGCAAGGAGCGAGCTCCAGGACCGGGTGCGCATGGTGATGCAGG
TGGTGACCGCCGACCTGCAGATGGCGGGAGCCCGCTACTGGAATAGTGGCGACCAAAACCAAGCCTTCAGCCTCCCCCTT
CCCCCGCTTTCGGGAAGCGACGGAGGCCCTAAGGACACCCTAACCCTCTACTACGTCACGAGCCTCCGGGACCTGGCCTC
CGCCTGCCGCCGGGTGGACTACGGGTTTGAAGGGGACACCTTGCGTCGGAGCGACGTGAGCGCCACACCCACCTCGGGGA
GCGACTGCACCACCCCTTCCCCCAACTACCAACCCCTGGCCGAGGGCATCCTGGCCCTGGACATCCAATACCAGTGCAGC
GACGGCACCACCCGAAACACTCCGGACTGCGGCGCAAACGCCTACCCCCGCTCGGCCAAGGTTACGGTGGCGGGCTACTC
CCTGACCCCGGTGACCAACCCGGGCCCCGCCTCCCTCACCACCGTCACCGGAAAGACCCTCGCCTGCCCCCAGGGCCGGG
CCTGCTACGCCCTCACCCAGGAGGTCCTCATGCCCAACCTCAAGCCCCTGCCCACCCCCTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-23)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA3 Thermus thermophilus HB27

93.133

100

0.931

Multiple sequence alignment