Detailed information    

insolico Bioinformatically predicted

Overview

Name   clpE   Type   Regulator
Locus tag   AAVB74_RS16435 Genome accession   NZ_CP154920
Coordinates   3109639..3111738 (+) Length   699 a.a.
NCBI ID   WP_345806396.1    Uniprot ID   -
Organism   Bacillus subtilis strain FUA2232     
Function   repress competence development (at the early growth phase) (predicted from homology)   
Competence regulation

Genomic Context

Location: 3104639..3116738
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAVB74_RS16405 (AAVB74_16405) ykvN 3104714..3105070 (+) 357 WP_014417586.1 winged helix-turn-helix transcriptional regulator -
  AAVB74_RS16410 (AAVB74_16410) queF 3105577..3106074 (-) 498 WP_003218613.1 preQ(1) synthase -
  AAVB74_RS16415 (AAVB74_16415) queE 3106092..3106823 (-) 732 WP_029317718.1 7-carboxy-7-deazaguanine synthase QueE -
  AAVB74_RS16420 (AAVB74_16420) queD 3106816..3107265 (-) 450 WP_003232462.1 6-carboxytetrahydropterin synthase QueD -
  AAVB74_RS16425 (AAVB74_16425) queC 3107258..3107917 (-) 660 WP_021479407.1 7-cyano-7-deazaguanine synthase QueC -
  AAVB74_RS16430 (AAVB74_16430) - 3108230..3109273 (-) 1044 WP_345806395.1 hypothetical protein -
  AAVB74_RS16435 (AAVB74_16435) clpE 3109639..3111738 (+) 2100 WP_345806396.1 ATP-dependent protease ATP-binding subunit ClpE Regulator
  AAVB74_RS16440 (AAVB74_16440) motA 3112121..3112933 (+) 813 WP_003238984.1 flagellar motor stator protein MotA -
  AAVB74_RS16445 (AAVB74_16445) motB 3112905..3113690 (+) 786 WP_003232473.1 flagellar motor protein MotB -
  AAVB74_RS16450 (AAVB74_16450) mhqR 3113730..3114167 (-) 438 WP_003232475.1 MarR family transcriptional regulator MhqR -
  AAVB74_RS16455 (AAVB74_16455) kinD 3114360..3115880 (+) 1521 WP_015252216.1 sporulation kinase KinD -
  AAVB74_RS16460 (AAVB74_16460) - 3115908..3116339 (-) 432 WP_029726990.1 hypothetical protein -
  AAVB74_RS16465 (AAVB74_16465) spo0E 3116425..3116682 (-) 258 WP_003218598.1 aspartyl-phosphate phosphatase Spo0E -

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 77787.92 Da        Isoelectric Point: 5.1809

>NTDB_id=895153 AAVB74_RS16435 WP_345806396.1 3109639..3111738(+) (clpE) [Bacillus subtilis strain FUA2232]
MRCQHCHQNEATIRLNMQINSVHKQMVLCETCYNELTRKPSMSMGPQSFGFPFEQAFQPKEQSAAKQSGKKGLLDELAQN
ITNGAKAGLIDPVIGRDDEVARVIEILNRRNKNNPVLIGEPGVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVA
NTGIRGQFEERMKQLITELKERKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAAL
ERRFQPVMVQEPSIEQAILILQGIKDKYEAYHGVTFSDEAIKACVTLSSRYIQDRHLPDKAIDLLDEAGSKANLLIDELN
DEDAAERLTAIEAEKIKALEEENYELAAKLRDEELALEKKLNSSSAHTAVTVEAEHIQEIVEQKTGIPVGKLQADEQTKM
KELEAKLHERVIGQAAAVQKVAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKTLADELFGTKDAIIRLDMSEYME
KHAVSKIIGSPPGYVGHEEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTDSQGRTVSFKDTVIIMTSN
AGAGEKQTKVGFQSDDSVIEEQTLIDSLSMFFKPEFLNRFDSIIEFRSLEKEHLVKIVSLLLGELEETLAERGISLNVTD
EAKEKIAELGYHPSFGARPLRRTIQEWVEDEMTDLLLDNGEITSFHVILEDDKIKVRAK

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=895153 AAVB74_RS16435 WP_345806396.1 3109639..3111738(+) (clpE) [Bacillus subtilis strain FUA2232]
ATGCGTTGTCAACATTGTCATCAAAACGAGGCGACGATTCGCCTTAACATGCAAATAAATTCCGTTCATAAACAGATGGT
TCTTTGTGAAACTTGCTATAACGAACTGACCCGTAAACCTTCAATGAGTATGGGTCCTCAATCTTTCGGATTTCCGTTTG
AACAGGCATTCCAGCCGAAAGAACAGAGCGCAGCAAAACAAAGCGGAAAAAAAGGGCTGCTTGATGAGCTGGCTCAAAAT
ATTACAAACGGTGCTAAAGCCGGTCTCATTGACCCCGTCATCGGCCGTGATGATGAAGTGGCGCGAGTGATCGAAATTCT
AAACCGCCGCAACAAAAACAATCCGGTTCTTATTGGTGAGCCGGGTGTAGGGAAAACTGCCATCGCTGAAGGGCTCGCTT
TAAAAATTGCTGAAGGCGATGTTCCAAACAAACTGAAAAACAAAGAGCTATATTTGCTTGATGTTGCATCCCTTGTTGCA
AACACAGGGATCAGAGGCCAATTTGAGGAGAGAATGAAACAGCTGATCACTGAGTTGAAGGAACGAAAAAATGTCATTCT
GTTCATTGATGAAATTCACCTTCTCGTTGGCGCAGGCTCTGCAGAAGGATCAATGGACGCCGGCAACATTCTCAAACCGG
CCCTAGCCAGAGGCGAACTGCAAGTCATTGGCGCGACAACACTGAAAGAATATCGTCAAATCGAAAAAGATGCCGCGCTG
GAAAGACGTTTTCAGCCTGTCATGGTGCAGGAGCCTTCAATTGAACAGGCTATCCTCATTCTGCAAGGGATTAAAGACAA
ATACGAGGCGTACCATGGCGTAACATTCAGTGATGAAGCAATCAAAGCGTGTGTCACTTTATCATCCCGCTACATTCAGG
ACAGACACCTGCCGGATAAAGCAATTGATTTATTAGATGAAGCAGGTTCAAAAGCCAACCTGTTAATTGATGAACTGAAT
GATGAGGATGCCGCTGAACGCTTAACTGCAATTGAAGCCGAAAAAATAAAAGCCCTGGAAGAAGAAAATTACGAACTGGC
GGCAAAACTCCGTGATGAAGAACTCGCATTGGAGAAAAAACTGAACAGCTCCTCCGCTCATACTGCTGTCACTGTGGAAG
CTGAGCACATTCAGGAAATTGTTGAACAAAAAACAGGCATCCCTGTCGGCAAACTGCAGGCAGACGAACAAACGAAAATG
AAAGAACTCGAAGCAAAACTTCATGAACGCGTGATTGGACAAGCAGCCGCTGTTCAAAAAGTGGCAAAGGCGGTAAGACG
AAGCCGCGCCGGTTTAAAATCCAAAAACAGACCAGTCGGCTCCTTCCTCTTCGTCGGTCCTACCGGCGTAGGGAAAACAG
AGCTTTCTAAAACACTGGCAGATGAATTATTCGGCACAAAAGACGCTATTATCCGACTCGATATGAGCGAATACATGGAG
AAACACGCCGTATCTAAAATTATCGGTTCACCGCCTGGATATGTCGGCCATGAGGAAGCTGGACAATTAACTGAAAAAGT
GCGCCGCAATCCTTACAGCATTGTGTTGCTGGATGAGATTGAAAAAGCACACCCAGACGTTCAGCATATGTTCCTGCAAA
TTATGGAAGATGGCCGTCTGACAGACAGCCAAGGCAGAACTGTAAGCTTCAAAGACACAGTGATCATCATGACAAGTAAT
GCGGGTGCTGGTGAGAAACAAACGAAAGTCGGTTTCCAATCAGATGACAGTGTCATCGAAGAACAAACATTAATTGATTC
ACTGAGCATGTTCTTTAAACCTGAGTTCCTCAACCGTTTTGACAGCATTATTGAGTTCCGCTCATTGGAAAAAGAACATC
TTGTCAAAATCGTCAGCCTTCTTCTTGGAGAACTTGAAGAAACATTGGCTGAACGGGGCATTAGCTTGAATGTGACAGAT
GAAGCGAAAGAAAAAATCGCTGAGCTGGGCTACCACCCTTCATTCGGTGCACGTCCGCTTAGAAGAACCATCCAAGAATG
GGTTGAGGATGAAATGACCGATCTGCTGCTTGATAATGGCGAGATCACAAGTTTTCACGTGATTTTAGAAGATGATAAAA
TCAAAGTGCGAGCGAAATAA

Domains

Predicted by InterProScan.

AAA domain (Cdc48 subfamily) (AAA_2)

(437-603)

AAA lid domain (AAA_lid_9)

(253-352)

C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small)

(609-679)

ATPase family associated with various cellular activities (AAA) (AAA)

(116-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpE Streptococcus mutans UA159

56.662

100

0.602
  clpC Lactococcus lactis subsp. cremoris KW2

56.557

100

0.592
  clpE Streptococcus pneumoniae TIGR4

54.924

100

0.567
  clpE Streptococcus pneumoniae Rx1

54.924

100

0.567
  clpE Streptococcus pneumoniae D39

54.924

100

0.567
  clpE Streptococcus pneumoniae R6

54.924

100

0.567
  clpC Bacillus subtilis subsp. subtilis str. 168

54.016

90.844

0.491
  clpC Lactococcus lactis subsp. lactis strain DGCC12653

47.702

90.272

0.431
  clpC Streptococcus pneumoniae Rx1

45.732

93.848

0.429
  clpC Streptococcus pneumoniae D39

45.732

93.848

0.429
  clpC Streptococcus thermophilus LMD-9

45.038

93.705

0.422
  clpC Streptococcus pneumoniae TIGR4

46.519

90.415

0.421
  clpC Streptococcus thermophilus LMG 18311

44.58

93.705

0.418
  clpC Streptococcus mutans UA159

46.216

88.841

0.411
  clpA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

42.079

86.695

0.365