Detailed information NTG0895147

Bioinformatically predicted

Overview

• Name: dprA/cilB/dalA
• Type: Machinery gene
• Locus tag: R4710_RS00830
• Genome accession: NZ_CP137101
• Coordinates: 163149..163997 (-)
• Length: 282 a.a.
• NCBI ID: WP_000705304.1
• Uniprot ID: A0A0E7P1X9
• Organism: Streptococcus pneumoniae strain 16P2012-2
• Function: ssDNA binding; loading RecA onto ssDNA; competence shut-off (predicted from homology) DNA processing Competence regulation

Genomic Context

Location: 158149..168997

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R4710_RS00815	-	158388..158747 (-)	360	WP_317804838.1	YbaN family protein	-
R4710_RS00820	topA	158968..161055 (-)	2088	WP_050245250.1	type I DNA topoisomerase	-
R4710_RS00825	-	161222..162265 (-)	1044	WP_001812710.1	TIGR00341 family protein	-
R4710_RS00830	dprA/cilB/dalA	163149..163997 (-)	849	WP_000705304.1	DNA-processing protein DprA	Machinery gene
R4710_RS00835	-	164093..164782 (-)	690	WP_000643970.1	sugar phosphate nucleotidyltransferase	-
R4710_RS00840	-	164794..165672 (-)	879	WP_000837609.1	DMT family transporter	-
R4710_RS00845	licA	165662..166531 (-)	870	WP_000411210.1	choline kinase LicA	-
R4710_RS00850	-	166548..167570 (-)	1023	WP_000609885.1	ribitol-5-phosphate dehydrogenase	-
R4710_RS00855	-	167575..168282 (-)	708	WP_050208610.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 282 a.a.
• Molecular weight: 31076.75 Da
• Isoelectric Point: 5.4047

>NTDB_id=895147 R4710_RS00830 WP_000705304.1 163149..163997(-) (dprA/cilB/dalA) [Streptococcus pneumoniae strain 16P2012-2]
MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADISGCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDC
YPWDLSEIYDAPVLLFYKGNLDLLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRGVIVAEAKMRSGSLITCERAMEE
GRDVFAIPGSILDGLSDGCHHLIQEGAKLLTSGQDVLAEFEF

Nucleotide

• Length: 849 bp

>NTDB_id=895147 R4710_RS00830 WP_000705304.1 163149..163997(-) (dprA/cilB/dalA) [Streptococcus pneumoniae strain 16P2012-2]
ATGAAAATCACAAACTATGAAATCTATAAGTTAAAAAAATCAGGTTTGACCAATCAACAGATTTTGAAAGTGCTAGAATA
CGGTGAAAATGTTGATCAGGAGCTTTTGTTGGGTGATATTGCAGATATATCAGGTTGCCGTAATCCAGCCGTTTTTATGG
AACGTTATTTTCAGATAGACGATGCGCATTTGTCGAAAGAGTTTCAAAAATTTCCATCTTTCTCTATTTTAGATGACTGT
TATCCTTGGGATTTGAGTGAAATATATGATGCGCCTGTACTTTTATTTTACAAGGGAAATCTTGACCTCCTGAAATTCCC
GAAGGTAGCGGTCGTGGGCAGTCGTGCTTGTAGCAAACAGGGAGCTAAGTCAGTTGAAAAAGTCATTCAAGGCTTGGAAA
ATGAACTGGTTATTGTCAGTGGTCTGGCCAAGGGCATTGACACAGCAGCTCATATGGCAGCTCTTCAGAATGGCGGAAAA
ACCATTGCAGTGATTGGAACAGGACTGGATGTGTTTTATCCTAAAGCCAATAAACGCTTGCAAGACTACATCGGCAATGA
CCATCTGGTTCTAAGTGAATATGGACCTGGCGAACAACCTCTGAAATTTCATTTTCCTGCCCGTAATCGCATCATTGCTG
GACTTTGTCGTGGTGTGATTGTAGCAGAGGCTAAGATGCGTTCAGGTAGTCTCATTACGTGTGAGCGAGCAATGGAAGAA
GGACGCGATGTCTTTGCTATTCCTGGTAGCATTTTAGATGGACTATCAGACGGTTGCCATCATTTGATTCAAGAAGGAGC
AAAATTGCTCACCAGTGGGCAAGATGTTCTTGCGGAATTTGAATTTTAA

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (75-271)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (3-64)

3D structure

• AlphaFold DB: A0A0E7P1X9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	99.645	100	0.996
dprA/cilB/dalA	Streptococcus pneumoniae D39	99.645	100	0.996
dprA/cilB/dalA	Streptococcus pneumoniae R6	99.645	100	0.996
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	99.645	100	0.996
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	98.227	100	0.982
dprA/cilB/dalA	Streptococcus mitis SK321	97.518	100	0.975
dprA	Streptococcus mutans UA159	63.214	99.291	0.628
dprA	Lactococcus lactis subsp. cremoris KW2	55.357	99.291	0.55
dprA	Staphylococcus aureus N315	37.456	100	0.376