Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   LDO09_RS06630 Genome accession   NZ_AP024926
Coordinates   1234063..1234533 (+) Length   156 a.a.
NCBI ID   WP_143585821.1    Uniprot ID   -
Organism   Thermus thermophilus strain AA1-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1229063..1239533
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LDO09_RS06610 (TthAA11_13030) ilvN 1229526..1230038 (-) 513 WP_223965549.1 acetolactate synthase small subunit -
  LDO09_RS06615 (TthAA11_13040) ilvB 1230035..1231723 (-) 1689 WP_223965550.1 biosynthetic-type acetolactate synthase large subunit -
  LDO09_RS06620 (TthAA11_13060) - 1232236..1232925 (+) 690 WP_143584079.1 IS5-like element ISTth7 family transposase -
  LDO09_RS06625 - 1232935..1233897 (-) 963 Protein_1283 YpdA family putative bacillithiol disulfide reductase -
  LDO09_RS06630 (TthAA11_13070) pilA/pilA1 1234063..1234533 (+) 471 WP_143585821.1 GspH/FimT family protein Machinery gene
  LDO09_RS06635 (TthAA11_13080) - 1234589..1235062 (+) 474 WP_223965551.1 GspH/FimT family protein -
  LDO09_RS06640 (TthAA11_13090) pilA/pilA2 1235059..1235640 (+) 582 WP_223965552.1 type II secretion system protein Machinery gene
  LDO09_RS06645 (TthAA11_13100) pilA/pilA3 1235637..1236338 (+) 702 WP_223965553.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LDO09_RS06650 (TthAA11_13110) comZ 1236349..1238025 (+) 1677 WP_223965554.1 pilus assembly PilX N-terminal domain-containing protein Machinery gene
  LDO09_RS12200 - 1238243..1238353 (+) 111 Protein_1289 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  LDO09_RS06660 (TthAA11_13130) - 1239036..1239416 (+) 381 WP_223968480.1 DUF1640 domain-containing protein -

Sequence

Protein


Download         Length: 156 a.a.        Molecular weight: 17032.82 Da        Isoelectric Point: 11.4446

>NTDB_id=89478 LDO09_RS06630 WP_143585821.1 1234063..1234533(+) (pilA/pilA1) [Thermus thermophilus strain AA1-1]
MTRRGLTLLELLLVLGLLGVLLGLALPLLSPNRLALDQAARSLAAQVTRARLEAIRRNAFVGLQVFTEEAGGYLLFVDQN
ANRRYDPGEEFGAVRFGQGEWARVRLDPERSTLGNMPLLFDPRGIPAKPITATLVLTSGSATRKVVISQQGRARVE

Nucleotide


Download         Length: 471 bp        

>NTDB_id=89478 LDO09_RS06630 WP_143585821.1 1234063..1234533(+) (pilA/pilA1) [Thermus thermophilus strain AA1-1]
ATGACGCGGCGGGGCCTTACCCTTTTGGAGCTCCTGCTGGTCTTGGGCCTCCTGGGGGTCCTCCTAGGGCTGGCCCTTCC
ACTTCTTTCCCCCAACCGCCTCGCCCTGGACCAGGCCGCCCGCTCCCTCGCCGCCCAGGTGACCCGGGCAAGGCTCGAGG
CCATCCGCCGGAACGCCTTCGTGGGCCTGCAGGTGTTCACCGAAGAAGCGGGGGGCTATCTGCTCTTCGTGGACCAAAAC
GCGAACCGCCGCTACGACCCCGGGGAGGAGTTCGGCGCGGTCCGCTTCGGCCAGGGAGAGTGGGCCAGGGTGCGTTTGGA
TCCCGAGAGGAGCACCCTCGGCAACATGCCCCTCCTCTTTGACCCCCGGGGCATCCCGGCCAAACCCATCACCGCCACCC
TGGTCCTCACCTCAGGGAGCGCCACCCGCAAGGTAGTTATCTCCCAACAGGGCCGGGCCCGGGTGGAGTGA

Domains

Predicted by InterproScan.

Type II transport protein GspH (GspH)

(39-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Thermus thermophilus HB27

92.913

81.41

0.756

Multiple sequence alignment