Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA3   Type   Machinery gene
Locus tag   LDO09_RS06645 Genome accession   NZ_AP024926
Coordinates   1235637..1236338 (+) Length   233 a.a.
NCBI ID   WP_223965553.1    Uniprot ID   A0AAD1NXB7
Organism   Thermus thermophilus strain AA1-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1230637..1241338
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LDO09_RS06620 (TthAA11_13060) - 1232236..1232925 (+) 690 WP_143584079.1 IS5-like element ISTth7 family transposase -
  LDO09_RS06625 - 1232935..1233897 (-) 963 Protein_1283 YpdA family putative bacillithiol disulfide reductase -
  LDO09_RS06630 (TthAA11_13070) pilA/pilA1 1234063..1234533 (+) 471 WP_143585821.1 GspH/FimT family protein Machinery gene
  LDO09_RS06635 (TthAA11_13080) - 1234589..1235062 (+) 474 WP_223965551.1 GspH/FimT family protein -
  LDO09_RS06640 (TthAA11_13090) pilA/pilA2 1235059..1235640 (+) 582 WP_223965552.1 type II secretion system protein Machinery gene
  LDO09_RS06645 (TthAA11_13100) pilA/pilA3 1235637..1236338 (+) 702 WP_223965553.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LDO09_RS06650 (TthAA11_13110) comZ 1236349..1238025 (+) 1677 WP_223965554.1 pilus assembly PilX N-terminal domain-containing protein Machinery gene
  LDO09_RS12200 - 1238243..1238353 (+) 111 Protein_1289 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  LDO09_RS06660 (TthAA11_13130) - 1239036..1239416 (+) 381 WP_223968480.1 DUF1640 domain-containing protein -
  LDO09_RS06665 (TthAA11_13140) - 1239492..1240034 (+) 543 WP_223968482.1 Uma2 family endonuclease -
  LDO09_RS06670 (TthAA11_13150) - 1240057..1240356 (+) 300 WP_008631315.1 nucleotidyltransferase -
  LDO09_RS06675 (TthAA11_13160) - 1240343..1240705 (+) 363 WP_060384345.1 HepT-like ribonuclease domain-containing protein -
  LDO09_RS06680 (TthAA11_13170) - 1240739..1241296 (-) 558 WP_223968484.1 Uma2 family endonuclease -

Sequence

Protein


Download         Length: 233 a.a.        Molecular weight: 24905.32 Da        Isoelectric Point: 7.4615

>NTDB_id=89480 LDO09_RS06645 WP_223965553.1 1235637..1236338(+) (pilA/pilA3) [Thermus thermophilus strain AA1-1]
MKRGFTLVEVLVAMAILVVVLAVGVRYFASTSELARNTQARSELQDRVRMVMQVVTADLQMAGARYWNSGDQNQAFSLPL
PPLSGSDGGPKDTLTLYYVTSLRDLASACRRVDYGFEGDTLRRSDVSATPTSGSDCTTPSPNYQPLAEGILALDIQYQCS
DGTTRNTPDCGANAYPRSAKVTVAGYSLTPVTNPGPASLTTVTGKTLACPQGRACYALTQEVLMPNLKPLPTP

Nucleotide


Download         Length: 702 bp        

>NTDB_id=89480 LDO09_RS06645 WP_223965553.1 1235637..1236338(+) (pilA/pilA3) [Thermus thermophilus strain AA1-1]
ATGAAGCGCGGCTTCACCCTGGTGGAGGTCCTGGTGGCCATGGCCATCTTGGTGGTGGTCCTGGCCGTGGGCGTGCGCTA
CTTCGCAAGCACAAGCGAACTCGCCCGGAACACCCAGGCAAGGAGCGAGCTCCAGGACCGGGTGCGCATGGTGATGCAGG
TGGTGACCGCCGACCTGCAGATGGCGGGAGCCCGCTACTGGAATAGTGGCGACCAAAACCAAGCCTTCAGCCTCCCCCTT
CCCCCGCTTTCGGGAAGCGACGGAGGCCCTAAGGACACCCTAACCCTCTACTACGTCACGAGCCTCCGGGACCTGGCCTC
CGCCTGCCGCCGGGTGGACTACGGGTTTGAAGGGGACACCTTGCGTCGGAGCGACGTGAGCGCCACACCCACCTCGGGGA
GCGACTGCACCACCCCTTCCCCCAACTACCAACCCCTGGCCGAGGGCATCCTGGCCCTGGACATCCAATACCAGTGCAGC
GACGGCACCACCCGAAACACTCCGGACTGCGGCGCAAACGCCTACCCCCGCTCGGCCAAGGTTACGGTGGCGGGCTACTC
CCTGACCCCGGTGACCAACCCGGGCCCCGCCTCCCTCACCACCGTCACCGGAAAGACCCTCGCCTGCCCCCAGGGCCGGG
CCTGCTACGCCCTCACCCAGGAGGTCCTCATGCCCAACCTCAAGCCCCTGCCCACCCCCTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-23)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA3 Thermus thermophilus HB27

93.562

100

0.936

Multiple sequence alignment