Detailed information NTG0894359

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: AAVD18_RS03835
• Genome accession: NZ_CP154893
• Coordinates: 701921..704332 (+)
• Length: 803 a.a.
• NCBI ID: WP_061573883.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain Fad 11/2
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 696921..709332

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAVD18_RS03810 (AAVD18_03810)	-	698563..699438 (+)	876	WP_013352211.1	YicC/YloC family endoribonuclease	-
AAVD18_RS03815 (AAVD18_03815)	remA	699515..699784 (+)	270	WP_003154355.1	extracellular matrix/biofilm regulator RemA	-
AAVD18_RS03820 (AAVD18_03820)	gmk	699792..700406 (+)	615	WP_013352212.1	guanylate kinase	-
AAVD18_RS03825 (AAVD18_03825)	rpoZ	700410..700613 (+)	204	WP_003154346.1	DNA-directed RNA polymerase subunit omega	-
AAVD18_RS03830 (AAVD18_03830)	coaBC	700704..701924 (+)	1221	WP_013352213.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
AAVD18_RS03835 (AAVD18_03835)	priA	701921..704332 (+)	2412	WP_061573883.1	primosomal protein N'	Machinery gene
AAVD18_RS03840 (AAVD18_03840)	def	704356..704838 (+)	483	WP_061573884.1	peptide deformylase	-
AAVD18_RS03845 (AAVD18_03845)	fmt	704843..705796 (+)	954	WP_061573885.1	methionyl-tRNA formyltransferase	-
AAVD18_RS03850 (AAVD18_03850)	rsmB	705783..707126 (+)	1344	WP_061573886.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
AAVD18_RS03855 (AAVD18_03855)	rlmN	707130..708221 (+)	1092	WP_013352218.1	23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN	-
AAVD18_RS03860 (AAVD18_03860)	-	708227..708988 (+)	762	WP_013352219.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-

Sequence

Protein

• Length: 803 a.a.
• Molecular weight: 90944.00 Da
• Isoelectric Point: 8.8944

>NTDB_id=894359 AAVD18_RS03835 WP_061573883.1 701921..704332(+) (priA) [Bacillus amyloliquefaciens strain Fad 11/2]
MNVAEVIVDVSSKNIDRPFDYRIPDHLRGMIEVGMRVIVPFGPRKLQGFVTGLKDSSELNGGQMKEVEKLLDLTPVLTEE
LMQLSSWLSDKTLSFKITALQAMLPAALKAKYEKELKVVDEEALTPDIRQLFRNQKSLLYSDITDERILSALQKFVRKGS
IDVTYKVAQKTNKKMIRMIEAGADKEELARHADSLTKQASKQLAVLTYFIDGGAKISAAELCQKTDSSSATLKTLLKKGL
LTDSFEEVYRDPYQDRMFKKTEPLSLTEEQQAAFEPIKKAVADHVHHVFLLHGVTGSGKTEIYLQSIEKVLAKGKEAIVL
VPEISLTPQMVNRFKGRFGSQVAVMHSGLSTGEKYDEWRKIQRKEVKLVVGARSAIFAPFENLGMIIIDEEHESSYKQEE
MPRYHAKDVAIRRAEHHSCPVVLGSATPTLESFARAKKGVYELLPLKHRVNRQLMPDVSLVDMREELRNGNRSMFSKELM
EQLEEKLAKKEQAVLFLNKRGYSSFVMCRDCGYVPQCPHCDISMTYHRYGQRLKCHYCGHEEPVPGTCPECGSEHIRFFG
TGTQRVEEELTKVLPQARVIRMDVDTTSRKGAHEKLLSAFGEGKADILLGTQMIAKGLDFPNVTLVGVLSADTTLHIPDF
RAAEKTFQLLTQVSGRAGRHEKPGTVVIQTYTPSHYSIQLTKTHDYETFFQQEMAHRRAQSYPPYYYVALVTVSHEEAAK
AAVTAEKITNYLRAGCGPDTKILGPAASPIARIKDRYRYQCVIKYRQENELQVQLKKILEHYKRDIAQKHVMISIDMNPY
MLM

Nucleotide

• Length: 2412 bp

>NTDB_id=894359 AAVD18_RS03835 WP_061573883.1 701921..704332(+) (priA) [Bacillus amyloliquefaciens strain Fad 11/2]
ATGAATGTGGCAGAAGTCATTGTCGATGTCAGCTCCAAAAACATTGACAGGCCCTTTGATTACAGAATTCCCGATCATTT
AAGAGGGATGATCGAAGTCGGCATGCGGGTGATTGTCCCGTTCGGCCCGCGCAAGCTTCAAGGGTTCGTGACCGGTTTGA
AAGATTCGTCTGAATTAAACGGCGGGCAAATGAAGGAAGTCGAGAAGCTTCTTGATTTGACACCCGTTTTGACGGAAGAG
CTGATGCAGCTGTCTTCATGGCTTTCTGACAAAACGCTGTCGTTTAAAATCACGGCGCTTCAGGCAATGCTGCCGGCCGC
GCTGAAGGCGAAGTACGAGAAAGAGCTGAAAGTCGTTGACGAAGAAGCGCTGACACCGGACATCAGGCAGCTTTTCAGAA
ATCAGAAGAGCCTTCTGTACTCAGATATTACCGATGAACGCATTCTTTCGGCACTGCAAAAATTCGTGCGCAAAGGAAGC
ATTGATGTCACCTATAAAGTCGCCCAGAAAACGAATAAAAAAATGATCCGCATGATTGAGGCGGGGGCAGACAAAGAAGA
ACTCGCCCGGCACGCAGATTCATTGACAAAACAGGCTTCTAAACAGCTTGCCGTGCTTACTTATTTTATTGACGGTGGAG
CAAAGATTTCCGCGGCTGAGCTTTGCCAAAAGACCGATTCCAGTTCAGCGACGCTGAAAACGCTTCTTAAAAAGGGGCTT
CTTACGGATAGCTTTGAGGAGGTTTACCGCGATCCTTATCAGGACAGAATGTTCAAAAAAACAGAACCTCTCTCTCTTAC
TGAAGAGCAACAGGCCGCTTTTGAGCCGATTAAGAAGGCCGTAGCCGATCATGTTCATCACGTCTTTCTGCTTCACGGTG
TGACAGGGAGCGGCAAAACGGAAATTTACCTGCAATCTATTGAAAAGGTGCTGGCTAAAGGAAAAGAAGCCATCGTGCTC
GTTCCGGAAATCTCATTGACGCCGCAAATGGTCAATCGTTTTAAAGGCCGCTTCGGTTCACAGGTTGCGGTCATGCACAG
CGGATTGTCCACAGGTGAAAAATACGATGAATGGCGGAAAATTCAGCGTAAGGAAGTAAAGCTTGTCGTCGGAGCCAGGT
CGGCCATCTTTGCCCCATTTGAAAATCTCGGCATGATCATTATTGATGAAGAGCACGAATCCTCCTATAAGCAGGAGGAA
ATGCCGCGGTACCATGCAAAAGACGTAGCCATACGCAGGGCCGAGCATCACAGCTGTCCGGTCGTCCTCGGGAGCGCCAC
GCCGACATTGGAGTCATTCGCACGGGCGAAAAAAGGCGTTTATGAGCTGCTCCCGCTGAAACACCGCGTAAATCGCCAGC
TCATGCCGGATGTATCTCTCGTTGATATGAGAGAAGAACTGCGAAACGGAAACAGATCGATGTTTTCAAAAGAGCTGATG
GAACAGCTGGAGGAGAAGCTTGCGAAAAAAGAGCAGGCCGTTCTCTTTCTGAATAAACGGGGATATTCTTCGTTTGTGAT
GTGCCGGGACTGCGGTTATGTGCCCCAATGTCCGCATTGCGACATTTCAATGACCTATCACCGCTACGGACAAAGGCTCA
AATGCCATTACTGCGGTCATGAGGAGCCTGTTCCGGGAACGTGCCCGGAATGCGGCAGTGAGCATATCCGCTTTTTCGGC
ACGGGAACGCAGCGTGTAGAGGAGGAGCTTACCAAAGTGCTGCCTCAGGCGCGCGTGATCAGGATGGATGTGGACACGAC
CTCAAGAAAAGGCGCTCATGAAAAGCTATTGTCAGCTTTCGGTGAAGGAAAAGCGGATATCCTGCTCGGTACGCAAATGA
TCGCAAAAGGGCTTGATTTTCCGAACGTGACGCTCGTCGGAGTATTAAGCGCAGATACGACGCTTCACATCCCTGATTTC
AGGGCGGCGGAGAAGACATTTCAGCTGTTGACGCAGGTAAGCGGACGGGCCGGGCGGCATGAAAAGCCGGGAACGGTAGT
CATACAAACGTACACACCGTCTCATTACAGCATTCAGCTGACGAAAACGCATGACTATGAAACTTTCTTTCAGCAGGAGA
TGGCGCACAGGCGGGCCCAGTCATATCCGCCTTACTATTATGTGGCGCTGGTGACCGTTTCTCATGAAGAAGCGGCAAAA
GCCGCGGTCACCGCTGAAAAGATTACAAACTATTTAAGAGCGGGATGCGGACCGGACACGAAAATTCTCGGGCCGGCGGC
TTCCCCGATTGCCAGGATCAAAGATAGATATCGCTATCAATGCGTGATAAAATACAGACAGGAAAACGAGTTGCAGGTTC
AGTTGAAAAAAATTCTGGAGCATTATAAACGCGACATTGCGCAAAAGCATGTAATGATTTCAATTGATATGAATCCGTAC
ATGCTGATGTAA

Domains

Predicted by InterProScan.

• Helicase conserved C-terminal domain (Helicase_C) (562-660)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (517-543)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-105)

• Primosomal protein N C-terminal domain (PriA_C) (704-799)

• Type III restriction enzyme, res subunit (ResIII) (265-429)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	83.727	100	0.839