Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   R3J35_RS10480 Genome accession   NZ_CP136972
Coordinates   2284555..2285967 (-) Length   470 a.a.
NCBI ID   WP_020851962.1    Uniprot ID   A0A9Q4QU34
Organism   Xylella fastidiosa subsp. multiplex strain CFBP8173     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 2279555..2290967
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R3J35_RS10460 (R3J35_10450) - 2279697..2280143 (+) 447 WP_027700144.1 pilin -
  R3J35_RS10465 (R3J35_10455) - 2280388..2280615 (-) 228 WP_012338102.1 hypothetical protein -
  R3J35_RS10470 (R3J35_10460) - 2281448..2281888 (+) 441 WP_012338104.1 pilin -
  R3J35_RS10475 (R3J35_10465) pilB 2282728..2284461 (+) 1734 WP_012338105.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R3J35_RS10480 (R3J35_10470) pilR 2284555..2285967 (-) 1413 WP_020851962.1 sigma-54 dependent transcriptional regulator Regulator
  R3J35_RS10485 (R3J35_10475) - 2286154..2287764 (-) 1611 WP_004084601.1 PAS domain-containing sensor histidine kinase -
  R3J35_RS10490 (R3J35_10480) sucC 2288019..2289182 (+) 1164 WP_004084603.1 ADP-forming succinate--CoA ligase subunit beta -
  R3J35_RS10495 (R3J35_10485) sucD 2289211..2290083 (+) 873 WP_004084605.1 succinate--CoA ligase subunit alpha -

Sequence

Protein


Download         Length: 470 a.a.        Molecular weight: 51368.35 Da        Isoelectric Point: 7.2158

>NTDB_id=893919 R3J35_RS10480 WP_020851962.1 2284555..2285967(-) (pilR) [Xylella fastidiosa subsp. multiplex strain CFBP8173]
MNESRSALIVDDERDIRELLVLTLGRMGLRIRTAANLAQAYALLESDSFNLCITDMRLPDGSGIELVKKIVRHYPRTPVA
MITAFGSIELAVEALKEGAFDFVSKPVDLNVLRGMVRHALELNNRERSVVSSQPQSPSQRLLGESSPMAQLRATIAKVAR
SQAPVYILGESGVGKELVARTIHEQSVRAAGAFVPVNCGAIPVDLMESEFFGHKKGSFTGAHTDKPGLFQSAGGGTLFLD
EVAELPLHMQVKLLRAIQEKSIRPIGATVEVPVDVRILSATHQDLSDLIANGRFRHDLYYRINVIEIRVPPLRERVGDLP
HLAAAIVARLAHTHGRAVPLLSPSALEALDHYSFPGNVRELENILERALALTEDDHIGVADLHLPQGSLHGQGLPLPLVP
TVLPIVPVDASALVEGENTPAALPSYMEQLERAAIQKALEENRWNKTKTAAKLGITFRALRYKLKKLGME

Nucleotide


Download         Length: 1413 bp        

>NTDB_id=893919 R3J35_RS10480 WP_020851962.1 2284555..2285967(-) (pilR) [Xylella fastidiosa subsp. multiplex strain CFBP8173]
ATGAATGAATCCCGCAGTGCTCTGATTGTCGATGACGAGCGCGACATCCGTGAACTTTTGGTCCTTACGCTAGGACGTAT
GGGGCTGCGTATCAGAACGGCAGCTAATCTGGCACAAGCATACGCGTTACTTGAGTCTGACTCGTTCAATCTTTGTATCA
CCGACATGCGTTTACCTGACGGCAGTGGCATTGAGCTGGTGAAAAAGATTGTGCGTCATTATCCACGTACCCCGGTGGCC
ATGATCACTGCTTTTGGCAGTATCGAACTGGCGGTAGAGGCGCTCAAGGAAGGTGCTTTTGACTTTGTCAGCAAACCTGT
TGATCTGAACGTCCTGCGAGGCATGGTCCGTCATGCTTTGGAATTGAACAATCGTGAACGCTCTGTTGTGTCAAGTCAGC
CACAAAGCCCCAGCCAACGTCTCCTTGGTGAATCATCACCGATGGCTCAGCTTCGGGCCACTATCGCTAAAGTGGCGCGT
AGTCAAGCCCCGGTTTATATCCTTGGTGAATCAGGAGTTGGTAAAGAACTGGTTGCACGTACGATCCACGAACAGAGTGT
GCGTGCTGCTGGGGCGTTCGTGCCGGTCAACTGTGGTGCGATCCCTGTGGATCTTATGGAAAGCGAATTTTTCGGTCACA
AGAAAGGGAGTTTCACCGGTGCGCATACTGATAAGCCAGGTTTATTCCAAAGTGCTGGCGGCGGTACTTTATTCCTGGAT
GAAGTTGCCGAATTACCCTTGCACATGCAAGTTAAATTGCTGCGTGCGATTCAGGAGAAATCGATACGGCCAATCGGTGC
AACCGTTGAGGTACCAGTAGATGTGCGTATTTTATCGGCGACTCACCAAGATCTGAGTGACCTGATTGCCAATGGCCGTT
TCCGTCACGACTTGTACTACCGCATCAATGTGATCGAGATACGTGTTCCACCGCTACGTGAGCGTGTTGGCGATTTGCCG
CATCTTGCTGCAGCCATTGTGGCTCGCTTGGCTCACACGCATGGGCGGGCGGTTCCGTTGCTTTCACCCTCCGCCTTGGA
GGCGTTAGATCATTATTCGTTCCCGGGTAATGTGCGTGAACTGGAGAACATTCTGGAGCGTGCTCTAGCACTGACTGAAG
ATGATCATATCGGTGTCGCGGATCTGCACCTGCCCCAGGGCAGTTTGCATGGACAGGGATTGCCGCTCCCGTTAGTGCCC
ACAGTTCTTCCGATTGTGCCTGTGGACGCATCGGCATTGGTGGAGGGTGAGAATACGCCGGCTGCCTTGCCGTCGTACAT
GGAGCAGCTGGAACGTGCTGCCATTCAAAAGGCGTTGGAAGAAAATCGCTGGAATAAAACAAAGACAGCCGCAAAACTTG
GCATCACTTTCCGTGCATTGCGTTACAAACTAAAGAAGCTGGGGATGGAGTGA

Domains

Predicted by InterproScan.

Bacterial regulatory protein, Fis family (HTH_8)

(427-467)

Sigma-54 interaction domain (Sigma54_activat)

(142-307)

Response regulator receiver domain (Response_reg)

(8-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

58.635

99.787

0.585
  pilR Acinetobacter baumannii strain A118

46.822

100

0.47