Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   R1T10_RS10040 Genome accession   NZ_CP136807
Coordinates   2000801..2001400 (+) Length   199 a.a.
NCBI ID   WP_002221479.1    Uniprot ID   A1KS89
Organism   Neisseria meningitidis NMB     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1995801..2006400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R1T10_RS10025 yihA 1996385..1997014 (+) 630 WP_002217904.1 ribosome biogenesis GTP-binding protein YihA/YsxC -
  R1T10_RS10030 - 1997135..1999531 (-) 2397 WP_002221477.1 penicillin-binding protein 1A -
  R1T10_RS10035 - 1999683..2000798 (+) 1116 WP_002214517.1 type IV pilus biogenesis protein PilM -
  R1T10_RS10040 pilN 2000801..2001400 (+) 600 WP_002221479.1 PilN domain-containing protein Machinery gene
  R1T10_RS10045 - 2001401..2002048 (+) 648 WP_002217908.1 type 4a pilus biogenesis protein PilO -
  R1T10_RS10050 pilP 2002066..2002611 (+) 546 WP_002214522.1 pilus assembly protein PilP Machinery gene
  R1T10_RS10055 pilQ 2002630..2004915 (+) 2286 WP_002214524.1 type IV pilus secretin PilQ Machinery gene
  R1T10_RS10060 - 2005877..2006389 (+) 513 WP_002214526.1 shikimate kinase -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 22243.45 Da        Isoelectric Point: 9.5493

>NTDB_id=892869 R1T10_RS10040 WP_002221479.1 2000801..2001400(+) (pilN) [Neisseria meningitidis NMB]
MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYLFIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQE
KDAFLIKKNKVEELQLKRLQAAKILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN

Nucleotide


Download         Length: 600 bp        

>NTDB_id=892869 R1T10_RS10040 WP_002221479.1 2000801..2001400(+) (pilN) [Neisseria meningitidis NMB]
ATGAACAATTTAATCAAAATCAACCTCCTCCCCTACAGGGAAGAGATGAACAAGCGCAAACAGCAGCAGTTTAAAACGCT
GATGTACGGTGCCGTGCTGACGGGCGTTGCCGCCGTTGCGGCAACCTACCTGTTTATCGACAATATGATCAATAACCAGT
CGGAAAGAAACACGCTGCTGGAAACCTCCATCGCACACTTGGATACCGAGCTGTCGGAAATACAAAAGCTCAAACAGGAA
AAAGATGCCTTCCTGATTAAGAAAAACAAAGTCGAGGAGCTCCAGCTCAAACGCCTCCAAGCCGCAAAAATCCTCGACAG
CCTGAATGAGGCCGTCCCCGGAAGCACCTACCTGACCTCGCTGGATGCCGTTACCGCCGACTCTTATCGGCTCAGCGGCA
GGACATCCAGCGACAACCGCGTTGCCGCCATGATGAGGGCGATGCCCAATACCGGCATATTCAAGCAACCCGAATTGTTA
AGCATCAAGAAAAACAATTCGCATCAAGAATTTACCCTTCAGGCAACATTACAACCCATCGTAAAGGCGGCCGAATCCAA
AGAGAATCCGGCTTCGGGAAACGCACAGGAGGCAAACTGA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(104-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A1KS89

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Neisseria meningitidis strain C311

99.497

100

0.995