Detailed information NTG0892118

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: RZ534_RS05460
• Genome accession: NZ_CP136643
• Coordinates: 1077956..1081660 (+)
• Length: 1234 a.a.
• NCBI ID: WP_317329019.1
• Uniprot ID: -
• Organism: Bacillus altitudinis strain RS1
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1072956..1086660

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RZ534_RS05445 (RZ534_05445)	-	1073254..1073664 (+)	411	WP_073415055.1	DoxX family protein	-
RZ534_RS05450 (RZ534_05450)	-	1073856..1074302 (-)	447	WP_007499129.1	ferritin-like domain-containing protein	-
RZ534_RS05455 (RZ534_05455)	addB	1074469..1077978 (+)	3510	WP_317329018.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
RZ534_RS05460 (RZ534_05460)	addA	1077956..1081660 (+)	3705	WP_317329019.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
RZ534_RS05465 (RZ534_05465)	sbcD	1081686..1082861 (+)	1176	WP_008348619.1	exonuclease subunit SbcD	-
RZ534_RS05470 (RZ534_05470)	sbcC	1082858..1086253 (+)	3396	WP_317329020.1	exonuclease subunit SbcC	-
RZ534_RS05475 (RZ534_05475)	-	1086271..1086570 (+)	300	WP_317329021.1	HNH endonuclease	-

Sequence

Protein

• Length: 1234 a.a.
• Molecular weight: 142616.51 Da
• Isoelectric Point: 5.1509

>NTDB_id=892118 RZ534_RS05460 WP_317329019.1 1077956..1081660(+) (addA) [Bacillus altitudinis strain RS1]
MQIPKPNNSTWTDDQWNAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEQPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELSKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPAPDQWMRAFLSLYDVDTQTKVEELPFYPYIKEDLSLVLRSCQELLEQA
LTLSKEPGGPAPRAENFIDDLEQVNELIRHQDDFGTLYELLPNINFKRLKTCKGDDYDPALLEKATDARNQAKKQLEKLK
DEYFMRSPDQHLKSLAEMKPVVETLVELVIQYGERFERAKQEKSIVDFSDLEHYCLRILAKQDAEGNLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGKHPAEGNRFMVGDVKQSIYRFRLAEPMLFLNKYKQFKADSNETGKRIDLNKNFR
SRADVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPQSEDTTTEMLLVHLDQHDTESDEEREELETVQFEARVIAKK
IKELVEQPFQVYDAKQQLTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPIVHLDENEMALIRTSDKKGTYYDAVKAFMSVTHSDHPTCRKLERFFLQLRKWRDFSIQHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLIAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAAKGDDWLLPDFERYQARTYLDFIGPALIRHQAMSSLLEESREVVLSHPSSFSITFHQASDL
LKEDMSLEKQKQDEVVQALMEGVPVEGYGDYEEEVKERLSWRYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSIRKAH
ATLYERPAFMKKKTLTAAEQGTAMHTVMQHIPLPSNESYDESRVEELLHSLKEKDLLTDEQIQSINREGIVAFFSTAIGQ
KLRKADWVKREVSFSMVLPVKEVYSHIDAEGEPVLIQGMIDCLFEADGKLYLLDYKTDRVTGRFSGGLEEAEAVLKKRYE
TQISLYTAAVERLTNRTLEEKILYFFDGNLQISL

Nucleotide

• Length: 3705 bp

>NTDB_id=892118 RZ534_RS05460 WP_317329019.1 1077956..1081660(+) (addA) [Bacillus altitudinis strain RS1]
ATGCAAATACCAAAACCGAATAACAGTACTTGGACAGATGACCAATGGAACGCCATCGTTTCAGAAGGACAAGATATTCT
TGTTGCTGCGGCGGCAGGGTCAGGGAAAACAGCGGTCTTGGTTGAACGGCTGATTCGAAAAATGACTCGGCCGGAGCAGC
CAGTTGATGTGGACAGGCTGCTCGTAGTGACTTTTACCAATGCTTCAGCAGCAGAAATGAAGCACCGGATCACTGAAGCA
CTTGAAAAAGAACTATCTAAAAACCCTGGATCACTTCATATGCGGAGACAGCTGTCACTTATGAATCGTGCCAATATTTC
TACCTTGCACTCTTTTTGTTTGCAAGTTCTCCGTACCTTTTATTATGAGATTGACCTTGATCCAGGTTTTCGTTTGGCTG
ATCAAACAGAAGGAGAATTATTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGATGAGTATAAGGCTGGAAAGCCGTCA
TTCTTTGAACTCGTTGATCGTTACACATCTGATCGGCATGATTTAGATCTTCAATGGCTTGTCAAAAGAATTTATGAGTT
TTCTAGGTCACATCCAGCGCCCGACCAATGGATGCGGGCATTCCTTTCTCTTTATGATGTGGATACACAGACAAAGGTAG
AAGAATTACCCTTTTATCCATATATCAAAGAGGACCTTTCTCTCGTTTTAAGAAGCTGTCAGGAACTGCTGGAACAAGCG
CTCACTTTATCAAAAGAGCCGGGCGGTCCAGCACCGAGAGCAGAGAATTTTATAGATGATTTAGAGCAAGTAAATGAATT
AATAAGGCATCAAGATGATTTTGGGACACTTTATGAGCTTTTGCCAAACATCAATTTTAAAAGGTTAAAGACTTGCAAAG
GGGATGACTATGACCCGGCTCTGTTAGAAAAAGCAACAGATGCACGCAATCAAGCCAAAAAACAATTAGAAAAGCTAAAA
GATGAATACTTTATGCGCAGTCCCGATCAGCATTTAAAAAGCTTGGCTGAAATGAAGCCAGTTGTAGAAACACTTGTAGA
GCTTGTGATTCAATATGGAGAGCGTTTCGAGAGAGCAAAACAAGAAAAATCAATCGTAGACTTTTCTGATCTAGAACATT
ATTGTTTGCGCATTTTAGCGAAGCAGGACGCAGAAGGAAATCTCATTGAAACAGAAGCGGCTAAGTACTATCAACAGCAA
TTTGAAGAAGTACTCGTGGATGAATACCAGGATACAAACCTTGTCCAAGAAACGATTTTAAAGCTCGTGTCTAAAGGAAA
ACATCCTGCCGAAGGCAACCGCTTTATGGTTGGTGATGTGAAGCAGTCCATTTATCGCTTTAGACTTGCAGAGCCGATGC
TCTTTTTAAATAAATATAAACAATTTAAAGCAGACAGCAATGAAACAGGTAAAAGAATTGATCTAAATAAAAACTTCCGT
AGCCGGGCTGATGTTTTAGACAGTACCAACTTTCTGTTCAAACAGCTGATGGGAGAAACGGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCGCAAAGCGAAGACACGACGACAGAAATGCTTCTTGTTCATTTAG
ATCAGCACGACACAGAAAGCGATGAAGAACGAGAAGAGCTAGAGACGGTTCAATTTGAGGCGAGAGTGATTGCAAAGAAA
ATAAAAGAATTGGTAGAACAGCCTTTTCAAGTGTACGATGCAAAACAGCAGCTGACCCGCAACTTGCAGTATCGAGACAT
TGTGATCTTGCTTCGATCAATGCCGTGGGCTCCGCAAATGATGGAAGAGTTGAAGAAGCAGGGAATCCCTGTTTATGCGA
ATCTTTCCTCAGGTTATTTCGAAGCGACAGAAGTATCTGTCATTCTTTCTTTGTTAAAAGTGATTGATAATCCATATCAA
GATATTCCGCTCGCTGCTGTTTTAAGGTCACCTATTGTTCATTTAGACGAAAATGAGATGGCACTGATTAGAACCAGTGA
TAAAAAAGGCACTTATTATGATGCTGTGAAGGCATTTATGAGTGTGACACACTCTGATCATCCGACGTGCAGAAAGCTGG
AGCGTTTCTTCCTTCAATTACGCAAATGGCGTGATTTTTCGATTCAGCATTCGGTAGCAGAACTGATTTGGGAAGTGTAC
CGAGATACACAATATCTAGATTATGTCGGCGGGATGCCAGGAGGCAAGCAAAGACAGGCAAACCTTCGGGCTTTATATGA
TCGAGCGAAACAGTATGAAAAGGCTGCATTTAGAGGGCTGTTTCGATTTCTTCGATTTATTGAAAGAATGCAAGAGCGTG
GAGATGATCTTGGAGCGGCAAAAACGTTCAGTGAAACAGAGGATGTTGTCCGCATGATGACCATTCATAGCAGTAAAGGA
CTCGAATTCCCTGTTGTGTTTACTGCAGGACTTGGCAGGAACTTTAATATGATGGATCTAAATCAGTCCTACTTGCTCGA
TAAAGAACTAGGTTTTGGCAGCAAATATATTCATCCTGAGCTGAGAATTAGTTATGCAACATTACCGCTGATCGCGATGA
AAAAAAAGATGAGAAAAGAGTTATTATCTGAAGAACTGAGGGTTCTATATGTGGCGCTCACAAGGGCGAAGGAAAAATTA
TTTTTAGTCGGCTCTGTCAAAAATCAAGTGAAAGCTTTAAGCAAGTGGCAAAATGCCGCAAAAGGAGACGACTGGCTCCT
CCCTGATTTTGAACGATATCAAGCGAGAACATACTTAGATTTTATTGGCCCTGCCCTTATCCGTCATCAAGCGATGTCTT
CTCTTTTAGAAGAATCGAGAGAAGTCGTTCTCTCTCATCCATCATCATTTTCTATCACCTTTCATCAAGCGTCCGATCTT
CTAAAAGAAGACATGTCACTTGAAAAACAAAAACAAGACGAAGTGGTACAAGCATTAATGGAAGGGGTGCCTGTTGAAGG
ATATGGCGATTACGAGGAGGAAGTAAAAGAAAGACTTTCATGGCGTTATCCATATTTAGCGGCCTCCCAAGTAGGGACAA
AGCAGTCAGTTTCCGAGATCAAAAGAATGAAAGAAATACAGGATGAATACAGTGTGCCTTCTTCTATTCGAAAAGCTCAT
GCGACATTGTACGAACGACCGGCTTTTATGAAGAAAAAAACACTCACAGCCGCAGAACAAGGGACGGCCATGCATACAGT
CATGCAGCATATCCCTCTTCCTTCAAATGAATCATATGATGAATCTCGTGTAGAAGAGCTGCTTCATTCTTTAAAAGAGA
AAGACTTACTAACAGATGAACAAATCCAGTCCATCAATCGAGAAGGAATTGTGGCCTTCTTTTCTACTGCTATTGGTCAA
AAACTGCGGAAAGCGGACTGGGTGAAACGAGAAGTATCGTTTAGTATGGTTTTACCGGTTAAAGAGGTATACAGCCATAT
TGATGCAGAAGGTGAACCTGTGCTCATTCAAGGAATGATTGATTGTTTATTTGAAGCAGATGGCAAGCTCTATTTACTTG
ATTATAAAACAGATCGAGTGACTGGCAGATTCTCAGGTGGATTAGAGGAAGCTGAAGCGGTGTTAAAAAAACGCTATGAA
ACGCAAATATCGTTGTATACAGCAGCGGTAGAACGTTTAACAAACAGAACATTAGAGGAGAAAATCCTTTATTTCTTTGA
TGGAAATCTACAAATTTCTTTATAG

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (473-883)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-463)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1052-1226)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	66.936	100	0.671