Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RI844_RS08710 Genome accession   NZ_CP136600
Coordinates   2133710..2134753 (-) Length   347 a.a.
NCBI ID   WP_348398057.1    Uniprot ID   -
Organism   Thalassotalea fonticola strain S1-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2128710..2139753
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RI844_RS08695 (RI844_08695) - 2130197..2131753 (+) 1557 WP_348398054.1 tryptophan halogenase family protein -
  RI844_RS08700 (RI844_08700) - 2131777..2132505 (+) 729 WP_348398055.1 SapC family protein -
  RI844_RS08705 (RI844_08705) pilU 2132584..2133696 (-) 1113 WP_348398056.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RI844_RS08710 (RI844_08710) pilT 2133710..2134753 (-) 1044 WP_348398057.1 type IV pilus twitching motility protein PilT Machinery gene
  RI844_RS08715 (RI844_08715) - 2134790..2135473 (+) 684 WP_348398058.1 YggS family pyridoxal phosphate-dependent enzyme -
  RI844_RS08720 (RI844_08720) proC 2135557..2136372 (+) 816 WP_348398059.1 pyrroline-5-carboxylate reductase -
  RI844_RS08725 (RI844_08725) - 2136448..2136996 (+) 549 WP_348398060.1 YggT family protein -
  RI844_RS08730 (RI844_08730) - 2137097..2137540 (+) 444 WP_348398061.1 DUF4426 domain-containing protein -
  RI844_RS08735 (RI844_08735) - 2137650..2138240 (+) 591 WP_348398062.1 XTP/dITP diphosphatase -
  RI844_RS08740 (RI844_08740) hemW 2138271..2139428 (+) 1158 WP_348398063.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38836.42 Da        Isoelectric Point: 5.6254

>NTDB_id=891895 RI844_RS08710 WP_348398057.1 2133710..2134753(-) (pilT) [Thalassotalea fonticola strain S1-1]
MDITELLAFSVEHNASDLHLSTGMPPAIRVDGDVRKVNIPPLEDKDVNGLVYDIMTDRQRKEYEENLEVDFSFEVPNLAR
FRVNAFNQNRGPAAVFRTIPSKILSLDELGCPDIFRQISENPRGLVLVTGPTGSGKSTTLAAMVDYINESKRDHILTIED
PIEFVHENKQCLINQREVHRDTLSFNNALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
VDVFPGEEKDMVRSMLSESLRAVISQTLIKKVGGGRVAAHEIMIGVPAIRNLIREDKIAQMYSAIQTGMSHGMQTMDQCL
QNLLSRGLITREDAMHKAADKNQFKVY

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=891895 RI844_RS08710 WP_348398057.1 2133710..2134753(-) (pilT) [Thalassotalea fonticola strain S1-1]
ATGGATATTACCGAACTTTTAGCATTCAGTGTTGAACACAATGCTTCCGATTTACATTTATCTACTGGTATGCCACCAGC
CATTCGTGTTGATGGTGACGTACGTAAGGTTAACATCCCGCCGCTAGAAGACAAAGACGTTAATGGTTTAGTGTATGACA
TTATGACCGATCGTCAACGTAAAGAATACGAAGAAAATTTAGAAGTCGATTTCTCCTTTGAAGTGCCTAATTTAGCGCGC
TTTCGTGTAAACGCCTTTAACCAAAATCGAGGCCCAGCTGCGGTATTTCGTACTATTCCAAGTAAAATTTTGTCGTTAGA
TGAGCTAGGTTGTCCTGATATTTTCAGACAAATTTCTGAAAATCCACGTGGTTTAGTGCTTGTTACGGGGCCAACGGGTT
CGGGTAAGTCTACTACATTAGCCGCCATGGTTGATTATATTAATGAATCTAAGCGCGATCACATTTTAACTATTGAAGAT
CCGATTGAATTTGTTCATGAGAATAAACAATGTTTAATTAACCAACGTGAAGTTCATCGCGACACACTAAGTTTTAACAA
TGCTTTGCGTTCAGCATTGCGTGAAGATCCTGATGTAATACTTGTTGGTGAGATGCGTGATTTAGAAACTATTCGACTAG
CGATGACTGCAGCAGAAACTGGTCATCTGGTATTTGGTACGCTGCATACAACCTCAGCACCAAAAACTATTGACCGTATC
GTTGATGTATTTCCAGGTGAAGAAAAAGACATGGTACGTTCAATGCTCTCTGAGTCTTTACGCGCGGTTATTTCACAAAC
ATTAATCAAAAAAGTTGGTGGTGGTCGAGTTGCTGCTCATGAAATTATGATTGGTGTGCCAGCTATTCGAAATCTTATTC
GTGAAGATAAAATAGCGCAAATGTATTCAGCGATTCAAACCGGTATGTCGCATGGCATGCAAACGATGGATCAATGTTTA
CAAAACTTATTAAGCCGTGGCCTTATCACCCGTGAAGATGCAATGCATAAAGCTGCTGATAAAAACCAGTTTAAGGTTTA
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.035

99.135

0.764
  pilT Acinetobacter nosocomialis M2

76.453

99.135

0.758
  pilT Acinetobacter baumannii D1279779

76.163

99.135

0.755
  pilT Acinetobacter baumannii strain A118

76.163

99.135

0.755
  pilT Pseudomonas stutzeri DSM 10701

76.163

99.135

0.755
  pilT Acinetobacter baylyi ADP1

75.581

99.135

0.749
  pilT Legionella pneumophila strain Lp02

72.674

99.135

0.72
  pilT Legionella pneumophila strain ERS1305867

72.674

99.135

0.72
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.92

97.118

0.689
  pilT Vibrio cholerae strain A1552

70.92

97.118

0.689
  pilT Neisseria meningitidis 8013

69.208

98.271

0.68
  pilT Neisseria gonorrhoeae MS11

68.915

98.271

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.333

99.424

0.53
  pilU Vibrio cholerae strain A1552

41.21

100

0.412
  pilU Pseudomonas stutzeri DSM 10701

40.346

100

0.403
  pilU Acinetobacter baylyi ADP1

39.943

100

0.401