Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RZV16_RS14705 Genome accession   NZ_CP136578
Coordinates   3424902..3426032 (-) Length   376 a.a.
NCBI ID   WP_039404792.1    Uniprot ID   A0AB34PCL6
Organism   Xanthomonas cannabis strain DAR82727     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3419902..3431032
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RZV16_RS14675 (RZV16_14625) - 3420110..3421057 (-) 948 WP_039404804.1 aspartate carbamoyltransferase catalytic subunit -
  RZV16_RS14680 (RZV16_14630) ruvX 3421071..3421538 (-) 468 WP_228334037.1 Holliday junction resolvase RuvX -
  RZV16_RS14685 (RZV16_14635) - 3421531..3422097 (-) 567 WP_039404799.1 YqgE/AlgH family protein -
  RZV16_RS14690 (RZV16_14640) - 3422403..3422969 (+) 567 WP_228334036.1 DNA-3-methyladenine glycosylase I -
  RZV16_RS14695 (RZV16_14645) - 3423006..3423905 (+) 900 WP_228314138.1 DUF72 domain-containing protein -
  RZV16_RS14700 (RZV16_14650) - 3423918..3424592 (-) 675 WP_039566430.1 YitT family protein -
  RZV16_RS14705 (RZV16_14655) pilU 3424902..3426032 (-) 1131 WP_039404792.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RZV16_RS14710 (RZV16_14660) pilT 3426146..3427183 (-) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  RZV16_RS14715 (RZV16_14665) - 3427511..3428203 (+) 693 WP_047693785.1 YggS family pyridoxal phosphate-dependent enzyme -
  RZV16_RS14720 (RZV16_14670) proC 3428247..3429101 (+) 855 WP_228334035.1 pyrroline-5-carboxylate reductase -
  RZV16_RS14725 (RZV16_14675) - 3429658..3430080 (+) 423 WP_047693787.1 HU family DNA-binding protein -
  RZV16_RS14730 (RZV16_14680) - 3430183..3430815 (+) 633 WP_184556994.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41982.24 Da        Isoelectric Point: 6.8287

>NTDB_id=891665 RZV16_RS14705 WP_039404792.1 3424902..3426032(-) (pilU) [Xanthomonas cannabis strain DAR82727]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAVAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRGRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=891665 RZV16_RS14705 WP_039404792.1 3424902..3426032(-) (pilU) [Xanthomonas cannabis strain DAR82727]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGACCTGTTCATCACCTCCGGGAT
GCCGCCGGCGATCAAGGTGCATGGCAAGATCAGCCCGATTACGCAGACACCGTTGACCGCGCAGCAAAGCCGCGACCTGG
TGTTGAACGTGATGACGCCGTCGCAGCGCGAAGAGTTCGAAAAGACCCACGAGTGCAATTTCGCCATCGGCGTGTCCGGG
GTCGGGCGTTTCCGTGTGAGCTGCTTCTACCAGCGCAACCAGGTGGGCATGGTGCTGCGCCGGATCGAGACGCGCATTCC
CACCGTGGAAGAGCTGAGCCTGCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGTACCGGTAAATCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAGGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTGGGCATCGATACCGACAG
CTGGGAAAACGCGCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGTACCCGCGAGGGCA
TGGATCACGCCGTGGCCTTTGCCGAAACCGGCCACCTGGTACTGTGCACCCTGCACGCAAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGACCGGCGCAACCAGCTGTTGATGGATCTGTCGCTCAATCTCAAGGGCGTGGT
CGCGCAGCAGCTGATTCCGACCCCGGATGGTCGCGGCCGGCGCGTGGCGATGGAGATCATGCTGGGCACACCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GATCAGAGCCTGTTCGAGCTCTACCAGGCCGGCGAGATCAGCTATGAAGACGCCTTGCGCTACGCCGATTCGCAAAACGA
GGTGCGTTTGCGCATCAAGCTGTCGCAGGGCGGCGATGCCAAGACCTTGTCGCAGGGCCTGGATGGCGTGGAGATTGCCG
AAGTTCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.847

97.606

0.662
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.297

94.947

0.402
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367