Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   OCU30_RS10145 Genome accession   NZ_AP024887
Coordinates   2179144..2180013 (+) Length   289 a.a.
NCBI ID   WP_077315754.1    Uniprot ID   A0A1R4B955
Organism   Vibrio palustris strain CECT 9027     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2174144..2185013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU30_RS10125 nadC 2174684..2175571 (+) 888 WP_077315750.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OCU30_RS10130 - 2175791..2176204 (+) 414 WP_077315751.1 pilin -
  OCU30_RS10135 - 2176204..2177889 (+) 1686 WP_077315752.1 GspE/PulE family protein -
  OCU30_RS10140 - 2177886..2179097 (+) 1212 WP_077315753.1 type II secretion system F family protein -
  OCU30_RS10145 pilD 2179144..2180013 (+) 870 WP_077315754.1 prepilin peptidase Machinery gene
  OCU30_RS10150 coaE 2180015..2180632 (+) 618 WP_077315755.1 dephospho-CoA kinase -
  OCU30_RS10155 zapD 2180652..2181392 (+) 741 WP_077315756.1 cell division protein ZapD -
  OCU30_RS10160 yacG 2181413..2181613 (+) 201 WP_077315757.1 DNA gyrase inhibitor YacG -
  OCU30_RS10165 parC 2181697..2183955 (-) 2259 WP_077315758.1 DNA topoisomerase IV subunit A -

Sequence

Protein


Download         Length: 289 a.a.        Molecular weight: 32427.48 Da        Isoelectric Point: 7.6053

>NTDB_id=89149 OCU30_RS10145 WP_077315754.1 2179144..2180013(+) (pilD) [Vibrio palustris strain CECT 9027]
MTIFLYYPWLYPTFVTIFGVIIGSFLNVVIYRTPKIMEQEWRQDCADSFPEYGITLPTERITLSIPRSQCRHCHTPLRII
DNIPLISWICLRGRCHHCQTPISCRYPLVEALTAIMSYIVASHFGPTPYGIAALFFTFVLIAATFIDFDTLLLPDQLTLP
LLWAGITLALLGYSPISLADAVVGAMAGYLILWSLYWGFKLLTGKDGMGYGDFKLLAALGAWLGWQQLPAIILLSSVVGI
VFGLVQLRLQNKGIAAAFPFGPYLAIAGWVCLLWGHNIITWYLHTFLGW

Nucleotide


Download         Length: 870 bp        

>NTDB_id=89149 OCU30_RS10145 WP_077315754.1 2179144..2180013(+) (pilD) [Vibrio palustris strain CECT 9027]
GTGACTATTTTTCTTTACTATCCATGGCTCTACCCTACTTTTGTAACAATTTTTGGGGTCATTATCGGTAGCTTTCTTAA
TGTGGTGATTTATCGCACGCCTAAAATTATGGAGCAAGAGTGGCGTCAAGACTGCGCGGACAGCTTTCCTGAATATGGCA
TTACTCTACCAACTGAGCGTATCACCTTAAGTATACCTCGTTCACAATGCCGTCATTGTCATACGCCTTTACGCATTATT
GATAACATCCCTCTCATTAGTTGGATTTGCTTGAGAGGACGTTGCCATCACTGCCAAACCCCGATTTCATGTCGCTACCC
ATTGGTTGAGGCACTCACGGCCATAATGAGCTATATCGTCGCTAGCCACTTTGGGCCCACTCCCTATGGCATCGCCGCAC
TGTTTTTTACTTTTGTGCTTATTGCCGCAACGTTTATCGATTTTGATACCTTACTTTTACCAGATCAACTGACTCTTCCG
TTATTATGGGCAGGCATTACCTTAGCTTTGCTGGGATATTCACCGATTAGCTTAGCCGATGCCGTGGTCGGAGCTATGGC
AGGCTATTTAATATTGTGGAGTCTGTATTGGGGCTTTAAACTGCTAACTGGCAAAGACGGAATGGGTTATGGTGACTTTA
AATTACTCGCCGCCCTTGGCGCATGGTTAGGTTGGCAACAACTTCCGGCGATTATTTTACTTTCTTCTGTCGTCGGTATT
GTTTTCGGGCTGGTACAATTACGCTTACAAAACAAAGGAATTGCAGCCGCATTTCCGTTTGGGCCGTATTTAGCTATAGC
GGGGTGGGTTTGTCTGCTTTGGGGGCATAATATTATCACTTGGTATCTACACACGTTTTTAGGCTGGTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(135-244)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1R4B955

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

71.875

99.654

0.716
  pilD Vibrio campbellii strain DS40M4

70.139

99.654

0.699
  pilD Acinetobacter nosocomialis M2

53.506

93.772

0.502
  pilD Acinetobacter baumannii D1279779

53.137

93.772

0.498
  pilD Neisseria gonorrhoeae MS11

43.273

95.156

0.412

Multiple sequence alignment