Detailed information NTG0088923

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: OCU46_RS02365
• Genome accession: NZ_AP024866
• Coordinates: 482477..483355 (-)
• Length: 292 a.a.
• NCBI ID: WP_141269797.1
• Uniprot ID: A0A4Y3ILF2
• Organism: Vibrio comitans strain LMG 23416
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 477477..488355

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OCU46_RS02330	rpsP	478773..479021 (+)	249	WP_017027436.1	30S ribosomal protein S16	-
OCU46_RS02335	rimM	479057..479587 (+)	531	WP_141269803.1	ribosome maturation factor RimM	-
OCU46_RS02340	trmD	479609..480367 (+)	759	WP_102376298.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
OCU46_RS02345	rplS	480409..480762 (+)	354	WP_021712249.1	50S ribosomal protein L19	-
OCU46_RS02350	yacG	480874..481068 (-)	195	WP_065210205.1	DNA gyrase inhibitor YacG	-
OCU46_RS02355	zapD	481094..481834 (-)	741	WP_141269801.1	cell division protein ZapD	-
OCU46_RS02360	coaE	481878..482477 (-)	600	WP_141269799.1	dephospho-CoA kinase	-
OCU46_RS02365	pilD	482477..483355 (-)	879	WP_141269797.1	prepilin peptidase	Machinery gene
OCU46_RS02370	-	483435..484643 (-)	1209	WP_141269795.1	type II secretion system F family protein	-
OCU46_RS02375	-	484636..486183 (-)	1548	WP_141269793.1	GspE/PulE family protein	-
OCU46_RS02380	-	486180..486584 (-)	405	WP_141269791.1	pilin	-
OCU46_RS02385	nadC	486825..487712 (-)	888	WP_141269789.1	carboxylating nicotinate-nucleotide diphosphorylase	-
OCU46_RS02390	ampD	487810..488352 (+)	543	WP_141269787.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-

Sequence

Protein

• Length: 292 a.a.
• Molecular weight: 32474.83 Da
• Isoelectric Point: 7.6540

>NTDB_id=88923 OCU46_RS02365 WP_141269797.1 482477..483355(-) (pilD) [Vibrio comitans strain LMG 23416]
MLDFYFTHYPLLFPILATILGLIIGSFLNVVIHRLPIIMEREWQKEFAEAYPELKLKVPEGVFNLSLPHSRCPKCEAPIK
AIHNIPVIGWLSLKGRCRSCGDKISPRYPLIELLSGGLSLTIALLFEPSFYAVALLGFTWVLLCATFIDLDTMLLPDSLT
LPLMWAGLALSVFGISPLSLQDAVIGAMAGYLSLWSVYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQSLPLIILLSSLV
GLIFGIIQLRLKQQGVEKAFPFGPYLAIAGWVYLIVGNDIVSWYLSRYIGGY

Nucleotide

• Length: 879 bp

>NTDB_id=88923 OCU46_RS02365 WP_141269797.1 482477..483355(-) (pilD) [Vibrio comitans strain LMG 23416]
ATGCTCGATTTTTATTTTACGCACTACCCTTTGCTATTTCCGATACTCGCCACCATTCTTGGTTTGATCATCGGTAGCTT
TCTCAATGTAGTGATCCATCGACTTCCGATCATCATGGAGAGAGAGTGGCAGAAAGAATTTGCCGAAGCCTACCCTGAGC
TAAAACTTAAGGTACCCGAAGGGGTTTTTAACCTCAGTCTACCTCACTCACGTTGCCCTAAATGTGAAGCTCCAATTAAA
GCGATACATAACATTCCGGTTATCGGCTGGTTAAGCCTCAAAGGGCGCTGCCGTTCTTGTGGCGATAAGATCAGTCCAAG
ATACCCTCTTATCGAGCTGCTTTCTGGGGGGCTAAGCCTCACCATCGCTCTCTTGTTTGAACCAAGCTTTTACGCCGTCG
CATTACTCGGATTTACTTGGGTACTGCTCTGTGCCACCTTCATCGATTTAGACACCATGCTACTGCCAGATAGCTTGACC
TTACCTCTGATGTGGGCGGGTTTAGCTTTATCAGTATTTGGGATCAGCCCGCTATCACTGCAAGATGCAGTCATCGGTGC
AATGGCAGGCTATCTTTCTTTGTGGAGTGTCTACTGGTTATTCAAACTACTCACTGGAAAAGAAGGCATGGGATATGGTG
ACTTCAAGCTATTGGCGGCGCTAGGTGCGTGGTTAGGTTGGCAGTCCTTACCTCTTATCATTTTACTTTCTTCATTAGTT
GGCCTGATATTTGGCATTATTCAACTTAGACTGAAGCAGCAAGGCGTTGAAAAAGCCTTCCCCTTCGGACCATACCTAGC
GATAGCCGGTTGGGTTTACTTGATAGTGGGCAATGACATTGTGTCTTGGTACCTCAGTCGCTACATCGGGGGATACTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (138-246)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (19-125)

3D structure

• AlphaFold DB: A0A4Y3ILF2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	67.018	97.603	0.654
pilD	Vibrio cholerae strain A1552	65.157	98.288	0.64
pilD	Acinetobacter baumannii D1279779	49.466	96.233	0.476
pilD	Acinetobacter nosocomialis M2	49.466	96.233	0.476
pilD	Neisseria gonorrhoeae MS11	48.913	94.521	0.462