Detailed information NTG0088901

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: AABJ76_RS21915
• Genome accession: NZ_AP027673
• Coordinates: 4496052..4498250 (+)
• Length: 732 a.a.
• NCBI ID: WP_001325632.1
• Uniprot ID: A7ZUF0
• Organism: Escherichia coli strain PSS-07
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4491052..4503250

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AABJ76_RS21880 (VEE16_41710)	metJ	4491541..4491858 (+)	318	WP_000852812.1	met regulon transcriptional regulator MetJ	-
AABJ76_RS21885 (VEE16_41720)	yiiX	4492042..4492650 (+)	609	WP_000797341.1	YiiX family permuted papain-like enzyme	-
AABJ76_RS21890	-	4492795..4493470 (+)	676	Protein_4270	IS1 family transposase	-
AABJ76_RS21895 (VEE16_41750)	-	4493557..4493880 (-)	324	WP_001323627.1	RHS element protein dsORF-f3	-
AABJ76_RS21900 (VEE16_41760)	-	4493882..4495081 (-)	1200	Protein_4272	RHS repeat-associated core domain-containing protein	-
AABJ76_RS21905	-	4495169..4495477 (-)	309	Protein_4273	DUF6531 domain-containing protein	-
AABJ76_RS21910 (VEE16_41770)	rpmE	4495637..4495849 (-)	213	WP_000710769.1	50S ribosomal protein L31	-
AABJ76_RS21915 (VEE16_41780)	priA	4496052..4498250 (+)	2199	WP_001325632.1	primosomal protein N'	Machinery gene
AABJ76_RS21920 (VEE16_41790)	cytR	4498406..4499431 (+)	1026	WP_000644904.1	DNA-binding transcriptional regulator CytR	Regulator
AABJ76_RS21925 (VEE16_41800)	ftsN	4499523..4500482 (+)	960	WP_000068834.1	cell division protein FtsN	-
AABJ76_RS21930 (VEE16_41810)	hslV	4500575..4501105 (+)	531	WP_000208242.1	ATP-dependent protease subunit HslV	-
AABJ76_RS21935 (VEE16_41820)	hslU	4501115..4502446 (+)	1332	WP_001293343.1	HslU--HslV peptidase ATPase subunit	-

Sequence

Protein

• Length: 732 a.a.
• Molecular weight: 81715.99 Da
• Isoelectric Point: 8.9077

>NTDB_id=88901 AABJ76_RS21915 WP_001325632.1 4496052..4498250(+) (priA) [Escherichia coli strain PSS-07]
MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIGVVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRL
LLWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVAELEF
NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVLDLKGQKVQ
AGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQQHLRCHHCDSQRPVPRQCPSCG
STHLVPVGLGTEQLEQTLAPLFPDVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERRMMQLPPWTSHVIVR
AEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPKRGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRK
VKWVLDVDPIEG

Nucleotide

• Length: 2199 bp

>NTDB_id=88901 AABJ76_RS21915 WP_001325632.1 4496052..4498250(+) (priA) [Escherichia coli strain PSS-07]
ATGCCCGTTGCCCACGTTGCCTTGCCCGTTCCGCTTCCTCGTACCTTTGACTATCTGCTGCCAGAAGGCATGACGGTTAA
AGCCGGGTGTCGCGTGCGCGTGCCGTTTGGCAAACAGCAGGAGCGCATCGGGGTTGTGGTGTCAGTTAGCGATGTCAGTG
AGCTGCCGCTTAACGAGCTGAAAGCGGTAGTTGAAGTGCTGGACGTTGAGCCGGTATTTACTCACTCCGTCTGGCGGTTA
CTGCTCTGGGCGGCAGATTACTATCATCATCCGATTGGCGATGTGCTGTTTCATGCCTTGCCGATTTTACTGCGCCAGGG
GCGGCCTGCGGCGAACGCGCCGATGTGGTACTGGTTTGCCACTGAACAAGGTCAGGCGGTGGATCTTAACAGCCTGAAAC
GTTCGCCAAAGCAACAACAGGCGCTGGCGGCGTTACGGCAAGGCAAAATCTGGCGCGACCAGGTCGCGGAACTTGAATTT
AATGATGCCGCGTTGCAGGCGCTGCGCAAAAAAGGTCTGTGTGATTTAGCAAGTGAAACACCAGAGTTTAGCGACTGGCG
AACGAACTATGCCGTTTCTGGTGAGCGGTTGCGATTGAATACCGAACAGGCCACCGCCGTTGGCGCAATTCATAGCGCGG
CAGATACTTTTTCTGCCTGGCTGCTGGCGGGCGTTACCGGTTCCGGTAAAACGGAGGTTTATCTCAGTGTACTGGAAAAC
GTGCTCGCTCAGGGCAAACAGGCGCTGGTGATGGTGCCGGAAATCGGCCTGACACCGCAAACTATCGCCCGTTTTCGTGA
ACGGTTTAATGCCCCCGTGGAAGTTCTGCATTCCGGCCTGAACGACAGCGAGCGGCTTTCGGCGTGGTTGAAAGCGAAAA
ATGGCGAGGCGGCGATTGTGATCGGCACCCGCTCCGCGCTGTTTACGCCGTTTAAAAATCTCGGCGTGATTGTCATTGAT
GAAGAGCACGACAGCTCCTACAAGCAGCAGGAAGGCTGGCGCTATCATGCCCGCGACCTGGCGGTGTATCGTGCGCATAG
CGAGCAAATCCCGATTATTCTTGGCTCCGCAACGCCCGCGCTGGAAACGTTGTGCAACGTCCAGCAGAAAAAATACCGCC
TGCTGCGCCTGACGCGCCGGGCGGGGAATGCGCGTCCGGCAATTCAACACGTGCTGGATTTAAAAGGTCAGAAGGTGCAG
GCAGGTCTGGCTCCGGCGTTAATCACCCGTATGCGCCAGCATTTACAGGCCAACAATCAGGTTATCCTCTTTCTTAATCG
TCGGGGTTTTGCGCCTGCGCTACTGTGCCACGACTGCGGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGC
TGCATCAGGCGCAACAGCATTTGCGCTGTCACCACTGTGACAGTCAGCGTCCGGTGCCGCGCCAGTGCCCTTCCTGCGGT
TCCACGCACCTGGTACCCGTGGGGCTGGGCACCGAACAGCTTGAACAGACGCTTGCGCCGCTGTTCCCTGATGTGCCCAT
TTCTCGTATCGACCGCGATACCACCAGCCGCAAAGGGGCGCTGGAACAGCAACTGGCGGAAGTACATCGCGGCGGTGCGA
GGATTTTGATCGGCACACAAATGCTGGCGAAAGGTCACCACTTTCCGGATGTGACGCTGGTGGCGTTACTGGATGTGGAC
GGCGCGCTGTTTTCTGCCGATTTTCGCTCGGCAGAACGTTTCGCCCAGCTTTACACCCAGGTCGCCGGTCGTGCCGGGCG
TGCGGGTAAACAGGGCGAAGTGGTGCTGCAAACGCACCATCCGGAACATCCTCTGTTGCAAACGTTGCTCTATAAAGGCT
ACGACGCCTTTGCCGAACAGGCGCTGGCTGAGCGACGCATGATGCAGCTGCCGCCGTGGACCAGCCATGTGATTGTGCGT
GCGGAAGATCATAACAATCAGCATGCGCCGCTGTTCCTGCAACAACTGCGTAATCTGATCCTTTCCAGCCCGCTGGCAGA
CGACAAACTGTGGGTTCTCGGCCCGGTTCCGGCACTGGCACCTAAGCGTGGTGGCCGCTGGCGCTGGCAGATCTTGTTGC
AGCACCCTTCCCGCGTGCGCTTGCAACACATCATTAGTGGTACGCTGGCGCTTATTAATACAATACCGGATTCCCGCAAG
GTGAAATGGGTGTTGGATGTCGATCCCATCGAAGGCTAA

Domains

Predicted by InterProScan.

• Primosomal protein N C-terminal domain (PriA_C) (632-729)

• DEAD/DEAH box helicase (DEAD) (203-362)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (445-471)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (6-101)

• Helicase conserved C-terminal domain (Helicase_C) (522-589)

3D structure

• AlphaFold DB: A7ZUF0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	45.047	100	0.454