Detailed information NTG0888794

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: RND74_RS14515
• Genome accession: NZ_CP136135
• Coordinates: 3195454..3196299 (-)
• Length: 281 a.a.
• NCBI ID: WP_406641039.1
• Uniprot ID: -
• Organism: Pectobacterium brasiliense strain ZRIMU1702DQ
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3190454..3201299

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RND74_RS14490	-	3190656..3191426 (-)	771	WP_406641034.1	ABC transporter ATP-binding protein	-
RND74_RS14495	-	3191428..3192465 (-)	1038	WP_406641035.1	FecCD family ABC transporter permease	-
RND74_RS14500	-	3192462..3193526 (-)	1065	WP_406641037.1	ABC transporter substrate-binding protein	-
RND74_RS14505	-	3193836..3194861 (+)	1026	WP_014916091.1	LLM class flavin-dependent oxidoreductase	-
RND74_RS14510	ypdK	3195219..3195293 (+)	75	WP_099606229.1	membrane protein YpdK	-
RND74_RS14515	pilD	3195454..3196299 (-)	846	WP_406641039.1	prepilin peptidase	Machinery gene
RND74_RS14520	-	3196408..3197154 (-)	747	WP_406641041.1	type II secretion system protein N	-
RND74_RS14525	gspM	3197144..3197638 (-)	495	WP_406641043.1	type II secretion system protein GspM	-
RND74_RS14530	gspL	3197635..3198912 (-)	1278	WP_406641045.1	type II secretion system protein GspL	-
RND74_RS14535	gspK	3198909..3199895 (-)	987	WP_406641047.1	type II secretion system minor pseudopilin GspK	-
RND74_RS14540	gspJ	3199892..3200542 (-)	651	WP_406641049.1	type II secretion system minor pseudopilin GspJ	-
RND74_RS14545	gspI	3200542..3200907 (-)	366	WP_039284733.1	type II secretion system minor pseudopilin GspI	-

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 31431.31 Da
• Isoelectric Point: 5.7656

>NTDB_id=888794 RND74_RS14515 WP_406641039.1 3195454..3196299(-) (pilD) [Pectobacterium brasiliense strain ZRIMU1702DQ]
MDDLREFAQLFPAWWFGSLGVLGLIVGSFLNVVIYRLPIMLERRWRQDIELETGIGDSDPDTRYNLWWPSSSCPHCQQAI
AVKDNIPLFSWLWLRGRSRCCHQSVSVQYPLVEIITMLAFLTAGLLWLPGMALWGALILLSFLLVLTVIDIKTLLLPDVL
TLSLLWIGLLFNLSETFVPLNDAVVGAMAGYLSLWLLYWVFKYATGKEALGYGDFKLLAALGAWLGWQALPNLVLVAALS
GLVVTLIWRGLRKEDTAKPLAFGPWLAMGGVFGVIMNGFNL

Nucleotide

• Length: 846 bp

>NTDB_id=888794 RND74_RS14515 WP_406641039.1 3195454..3196299(-) (pilD) [Pectobacterium brasiliense strain ZRIMU1702DQ]
GTGGACGATTTAAGGGAATTTGCACAGCTGTTTCCGGCGTGGTGGTTTGGATCGCTGGGTGTGTTGGGATTGATTGTTGG
CAGTTTTCTGAACGTGGTGATTTACCGCTTGCCGATTATGCTGGAGCGTCGCTGGCGGCAGGACATCGAGCTTGAAACGG
GCATAGGCGATTCCGATCCTGATACGCGCTACAACCTGTGGTGGCCGTCTTCGTCGTGCCCACACTGCCAGCAGGCTATT
GCGGTGAAGGATAACATCCCGCTATTCAGTTGGCTGTGGTTGCGTGGCCGCTCTCGCTGTTGTCATCAATCGGTGTCTGT
GCAATATCCGCTAGTGGAAATCATCACCATGCTGGCTTTTCTGACGGCAGGTTTACTCTGGCTGCCCGGTATGGCGCTGT
GGGGGGCGTTGATTCTGCTGTCCTTCCTGTTGGTACTGACCGTTATTGATATCAAAACGCTGCTGCTGCCGGATGTGCTT
ACGCTGTCGCTTCTGTGGATAGGGCTGCTGTTTAATCTGTCGGAGACATTTGTCCCATTGAATGATGCGGTAGTCGGCGC
GATGGCTGGTTATTTATCTCTGTGGCTGCTTTATTGGGTATTCAAATACGCAACGGGAAAAGAGGCGCTGGGGTACGGTG
ATTTTAAGTTGCTGGCTGCGCTGGGTGCCTGGCTGGGCTGGCAGGCATTGCCGAATCTGGTTCTGGTGGCGGCGCTGAGT
GGGTTGGTCGTGACGCTTATTTGGCGCGGATTGCGTAAAGAGGATACGGCTAAACCGCTGGCCTTTGGCCCCTGGCTGGC
AATGGGCGGGGTGTTTGGCGTGATAATGAACGGATTCAATCTGTAG

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-123)

• Type IV leader peptidase family (Peptidase_A24) (137-245)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.558	95.73	0.484
pilD	Vibrio campbellii strain DS40M4	45.353	95.73	0.434
pilD	Neisseria gonorrhoeae MS11	46.743	92.883	0.434
pilD	Acinetobacter baumannii D1279779	42.491	97.153	0.413
pilD	Acinetobacter nosocomialis M2	42.125	97.153	0.409