Detailed information NTG0888773

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: RND66_RS15185
• Genome accession: NZ_CP136134
• Coordinates: 3430691..3431536 (-)
• Length: 281 a.a.
• NCBI ID: WP_119871904.1
• Uniprot ID: -
• Organism: Pectobacterium brasiliense strain ZRIMU1701SM
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3425691..3436536

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RND66_RS15160	-	3425893..3426663 (-)	771	WP_205594228.1	ABC transporter ATP-binding protein	-
RND66_RS15165	-	3426665..3427702 (-)	1038	WP_080724594.1	iron ABC transporter permease	-
RND66_RS15170	-	3427699..3428763 (-)	1065	WP_205594229.1	ABC transporter substrate-binding protein	-
RND66_RS15175	-	3429073..3430098 (+)	1026	WP_014916091.1	LLM class flavin-dependent oxidoreductase	-
RND66_RS15180	ypdK	3430456..3430530 (+)	75	WP_099606229.1	membrane protein YpdK	-
RND66_RS15185	pilD	3430691..3431536 (-)	846	WP_119871904.1	A24 family peptidase	Machinery gene
RND66_RS15190	-	3431645..3432391 (-)	747	WP_119871903.1	type II secretion system protein N	-
RND66_RS15195	gspM	3432381..3432875 (-)	495	WP_119871902.1	type II secretion system protein GspM	-
RND66_RS15200	gspL	3432872..3434149 (-)	1278	WP_205594230.1	type II secretion system protein GspL	-
RND66_RS15205	gspK	3434146..3435132 (-)	987	WP_039273741.1	type II secretion system minor pseudopilin GspK	-
RND66_RS15210	gspJ	3435129..3435740 (-)	612	WP_338327688.1	type II secretion system minor pseudopilin GspJ	-
RND66_RS15215	gspI	3435779..3436144 (-)	366	WP_039273738.1	type II secretion system minor pseudopilin GspI	-

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 31466.46 Da
• Isoelectric Point: 6.4557

>NTDB_id=888773 RND66_RS15185 WP_119871904.1 3430691..3431536(-) (pilD) [Pectobacterium brasiliense strain ZRIMU1701SM]
MDDLREFAQLFPAWWFGSLGVLGLIVGSFLNVVIYRLPIMLERRWRQDIELETGMADSDPDKRYNLWWPPSSCPHCQQAI
AVKDNIPLFSWLWLRGRSRCCHQSVSVQYPLVEVITMLAFLTAGLLWLPGMALWGALILLSFLLVLTVIDIKTLLLPDVL
TLSLLWIGLLFNLSETFVPLNDAVVGAMAGYLSLWLLYWVFKYVTGKEALGYGDFKLLAALGAWLGWQALPNLVLVAALS
GLVVTLVWRGLRKEDTAKPLAFGPWLAMGGVFGVIMNGLNL

Nucleotide

• Length: 846 bp

>NTDB_id=888773 RND66_RS15185 WP_119871904.1 3430691..3431536(-) (pilD) [Pectobacterium brasiliense strain ZRIMU1701SM]
GTGGACGATTTAAGGGAATTTGCACAGCTGTTTCCGGCATGGTGGTTTGGATCGCTGGGCGTGCTGGGATTGATTGTTGG
CAGTTTTTTGAACGTGGTGATTTACCGCTTGCCGATTATGCTGGAGCGTCGCTGGCGGCAGGACATCGAGCTTGAAACGG
GCATGGCCGATTCCGATCCTGATAAGCGCTACAACCTGTGGTGGCCGCCTTCGTCGTGCCCACACTGCCAGCAGGCTATT
GCGGTGAAGGATAACATCCCGCTATTCAGTTGGCTGTGGTTGCGTGGCCGCTCCCGCTGCTGTCATCAATCAGTATCTGT
GCAATATCCGTTAGTGGAAGTCATCACCATGCTGGCTTTTCTGACGGCAGGTTTACTCTGGCTACCCGGTATGGCGCTGT
GGGGGGCGTTGATTCTGCTGTCATTCCTGTTGGTGCTGACCGTTATTGATATAAAAACGCTGCTGCTGCCGGATGTGCTT
ACGCTGTCGCTTCTGTGGATAGGGCTGCTGTTTAATCTGTCGGAGACATTTGTCCCATTGAATGATGCCGTCGTCGGCGC
GATGGCCGGTTATTTATCTCTGTGGCTGCTTTATTGGGTATTCAAATACGTAACGGGCAAAGAGGCGCTGGGGTACGGTG
ATTTTAAGTTGCTGGCTGCGCTGGGTGCCTGGCTGGGCTGGCAGGCATTGCCGAATCTGGTTCTGGTGGCGGCGCTGAGC
GGGTTGGTCGTGACTCTCGTCTGGCGCGGATTGCGTAAAGAGGATACGGCTAAACCGCTGGCCTTTGGCCCCTGGCTGGC
AATGGGCGGGGTATTTGGCGTGATAATGAACGGATTGAATCTGTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-123)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.185	96.441	0.484
pilD	Neisseria gonorrhoeae MS11	47.126	92.883	0.438
pilD	Vibrio campbellii strain DS40M4	44.981	95.73	0.431
pilD	Acinetobacter baumannii D1279779	41.758	97.153	0.406
pilD	Acinetobacter nosocomialis M2	41.392	97.153	0.402