Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   RUI02_RS08710 Genome accession   NZ_CP136099
Coordinates   1656057..1656950 (+) Length   297 a.a.
NCBI ID   WP_014479775.1    Uniprot ID   A0AA96ZWR3
Organism   Bacillus sp. TSA-4     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1651057..1661950
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RUI02_RS08690 (RUI02_08690) - 1651727..1653457 (+) 1731 WP_046381021.1 hypothetical protein -
  RUI02_RS08695 (RUI02_08695) - 1653454..1653735 (+) 282 WP_003232001.1 FlhB-like flagellar biosynthesis protein -
  RUI02_RS08700 (RUI02_08700) sucC 1653908..1655065 (+) 1158 WP_003244732.1 ADP-forming succinate--CoA ligase subunit beta -
  RUI02_RS08705 (RUI02_08705) sucD 1655094..1655996 (+) 903 WP_003238566.1 succinate--CoA ligase subunit alpha -
  RUI02_RS08710 (RUI02_08710) dprA 1656057..1656950 (+) 894 WP_014479775.1 DNA-processing protein DprA Machinery gene
  RUI02_RS08715 (RUI02_08715) topA 1657138..1659213 (+) 2076 WP_046381022.1 type I DNA topoisomerase -
  RUI02_RS08720 (RUI02_08720) trmFO 1659289..1660596 (+) 1308 WP_003244725.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  RUI02_RS08725 (RUI02_08725) xerC 1660664..1661578 (+) 915 WP_003231988.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 297 a.a.        Molecular weight: 32907.21 Da        Isoelectric Point: 9.9142

>NTDB_id=888405 RUI02_RS08710 WP_014479775.1 1656057..1656950(+) (dprA) [Bacillus sp. TSA-4]
MDQAAACLTICRINQLLSPSLLLKWWKADPSMSLTSPVLQTVTRDQIKAAALKNEIEQFYPKLPRVLAAYREQGINTIPI
SSKQYPFWLKSIYDPPAVLFAKGDMTLLSKGRKIGIVGTRNPTAYGKQVVNHLTKEICRKGWVIVSGLASGIDGMSHAAS
IKAKGRTIGVIAGGFQHIYPRENLQLADHMAKHHILLSEHPPETKPQKWHFPMRNRIISGLSEGVIVVQGKEKSGSLITA
YQALEQGREVFAVPGSLFDPYAGGPIKLIQQGAKAIWSAEDIFEELPERNVQYTEPF

Nucleotide


Download         Length: 894 bp        

>NTDB_id=888405 RUI02_RS08710 WP_014479775.1 1656057..1656950(+) (dprA) [Bacillus sp. TSA-4]
TTGGATCAGGCCGCTGCCTGCCTAACGATTTGCAGAATCAATCAATTATTATCCCCATCCCTTCTATTAAAATGGTGGAA
AGCCGATCCGTCTATGTCGCTGACATCACCCGTGTTACAAACGGTTACTCGTGATCAAATAAAAGCAGCTGCATTAAAAA
ACGAAATAGAACAATTTTATCCAAAGCTCCCGCGTGTACTTGCTGCTTATCGTGAGCAAGGCATTAACACCATCCCTATT
TCTTCAAAGCAATATCCTTTCTGGCTTAAAAGCATTTATGATCCCCCTGCCGTACTGTTTGCAAAAGGTGATATGACTCT
TCTTTCGAAAGGGAGAAAAATTGGAATTGTAGGGACAAGAAATCCAACAGCTTATGGGAAACAAGTTGTCAATCATCTTA
CAAAAGAGATCTGTCGTAAAGGTTGGGTGATTGTCAGCGGACTGGCGTCTGGGATAGACGGAATGTCCCATGCTGCAAGT
ATTAAGGCGAAGGGACGGACAATTGGCGTCATTGCAGGCGGATTTCAACACATTTATCCCCGAGAAAACCTTCAGTTAGC
AGATCACATGGCTAAACACCATATCCTGCTGTCAGAGCACCCACCTGAAACTAAACCCCAAAAATGGCATTTCCCTATGA
GAAACCGTATTATCAGCGGACTAAGTGAAGGCGTTATTGTCGTTCAGGGCAAAGAAAAAAGCGGTTCGCTGATTACTGCC
TATCAAGCATTGGAACAAGGGAGAGAGGTATTTGCCGTACCCGGTTCATTGTTTGACCCTTACGCCGGAGGTCCTATAAA
ACTGATCCAGCAGGGGGCTAAAGCCATATGGTCAGCAGAGGATATTTTCGAGGAACTTCCTGAGAGAAACGTTCAATATA
CGGAACCCTTTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(71-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

99.663

100

0.997
  dprA Legionella pneumophila strain ERS1305867

38.462

100

0.387
  dprA Lactococcus lactis subsp. cremoris KW2

42.105

89.562

0.377