Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   RUI02_RS01965 Genome accession   NZ_CP136099
Coordinates   364571..365020 (-) Length   149 a.a.
NCBI ID   WP_003234608.1    Uniprot ID   A0A8E0S750
Organism   Bacillus sp. TSA-4     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 359571..370020
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RUI02_RS01925 (RUI02_01925) - 360015..360210 (+) 196 Protein_335 hypothetical protein -
  RUI02_RS01930 (RUI02_01930) - 360217..360564 (+) 348 WP_082098065.1 RDD family protein -
  RUI02_RS01935 (RUI02_01935) - 360646..360978 (+) 333 WP_015252849.1 YckD family protein -
  RUI02_RS01940 (RUI02_01940) - 361106..361528 (+) 423 WP_174616579.1 MarR family winged helix-turn-helix transcriptional regulator -
  RUI02_RS01945 (RUI02_01945) - 361516..361857 (+) 342 WP_032724911.1 DUF3147 family protein -
  RUI02_RS01950 (RUI02_01950) - 361876..362604 (+) 729 WP_046380777.1 AAA family ATPase -
  RUI02_RS01955 (RUI02_01955) bglC 362676..364109 (+) 1434 WP_046380778.1 6-phospho-beta-glucosidase -
  RUI02_RS01960 (RUI02_01960) nin/comJ 364146..364544 (-) 399 WP_015715226.1 DNA-entry nuclease inhibitor Regulator
  RUI02_RS01965 (RUI02_01965) nucA/comI 364571..365020 (-) 450 WP_003234608.1 DNA-entry nuclease Machinery gene
  RUI02_RS01970 (RUI02_01970) tlpC 365188..366909 (-) 1722 WP_046380779.1 methyl-accepting chemotaxis protein TlpC -
  RUI02_RS01975 (RUI02_01975) hxlB 367020..367577 (-) 558 WP_003234605.1 6-phospho-3-hexuloisomerase -
  RUI02_RS01980 (RUI02_01980) hxlA 367583..368215 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  RUI02_RS01985 (RUI02_01985) hxlR 368444..368806 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16470.42 Da        Isoelectric Point: 4.5282

>NTDB_id=888374 RUI02_RS01965 WP_003234608.1 364571..365020(-) (nucA/comI) [Bacillus sp. TSA-4]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSTDQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=888374 RUI02_RS01965 WP_003234608.1 364571..365020(-) (nucA/comI) [Bacillus sp. TSA-4]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGTCTGATAAA
AGGAGACTTTTTTTCAACTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGACACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98