Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   RS399_RS04875 Genome accession   NZ_CP135973
Coordinates   942459..946163 (-) Length   1234 a.a.
NCBI ID   WP_084991639.1    Uniprot ID   -
Organism   Bacillus inaquosorum strain SI3     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 937459..951163
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS399_RS04860 (RS399_04860) hlpB 937507..937809 (-) 303 WP_044427342.1 HNH nuclease-like protein HlpB -
  RS399_RS04865 (RS399_04865) sbcC 937823..941215 (-) 3393 WP_084991636.1 exonuclease subunit SbcC -
  RS399_RS04870 (RS399_04870) sbcD 941212..942387 (-) 1176 WP_084991638.1 exonuclease subunit SbcD -
  RS399_RS04875 (RS399_04875) addA 942459..946163 (-) 3705 WP_084991639.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  RS399_RS04880 (RS399_04880) addB 946150..949650 (-) 3501 WP_084991642.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  RS399_RS04885 (RS399_04885) - 949833..950270 (+) 438 WP_084991645.1 ferritin family protein -
  RS399_RS04890 (RS399_04890) - 950389..950718 (+) 330 WP_019257992.1 four-helix bundle copper-binding protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141265.62 Da        Isoelectric Point: 5.0946

>NTDB_id=887998 RS399_RS04875 WP_084991639.1 942459..946163(-) (addA) [Bacillus inaquosorum strain SI3]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITADENPIDVDRLLVVTFTNASAAEMKHRIADA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQFLVKQVYEYSRSHPNPEAWLESFVHLYDVSEKSSIEELPFYQYVKEDIAMVLNGAKEKLQRA
LELTKAPGGPAPRADNFFDDLAQIDELIQHQDDFTELYKRVPAVSFKRAKAVKGDEFDPALLEEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKILADMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLSILTAENDKGEREPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPDNDETETELLLIDNSEETDAGEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLMSGYFEAVEVAVALSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENTKAPYYEAMKDYLAAGDRNDELYQKLQTFYGHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPQLRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKDHQKQLAKWQATASQTDWLLPEFDRYQAKTYLDFIGPALARHRDLEALAENGAAAHADISGHPARFAVQMVHSYD
LLDDDLEERLEEKSERLEAIRRGEPVPGSFAFDEKAREQLSWTYPHHEVTQIRTKQSVSEIKRKREYEDEYSGRAPVKPA
DGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTIEEAKQTVHKLYEKELLTEEQKAAIDIEEIVQFFHTEIGG
QLIGAKWKDREVPFSLALPAKEIYPDAQEADEPLLVQGIIDCLYETEDGLYLLDYKSDRIEGKFQHGFEGAAPILKKRYE
TQIQLYTKAVEQIAKTQVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=887998 RS399_RS04875 WP_084991639.1 942459..946163(-) (addA) [Bacillus inaquosorum strain SI3]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAATGGAATGCCATTGTTTCAACCGGCCAGGATATTCT
TGTGGCAGCGGCGGCGGGCTCCGGTAAAACCGCTGTGCTCGTTGAACGAATGATCCGGAAAATCACCGCGGATGAAAACC
CGATTGATGTAGACCGGCTTCTCGTTGTGACATTTACAAACGCCTCAGCGGCAGAGATGAAACACCGGATCGCGGATGCC
CTGGAAAAAGAGCTTGTAAAGCGCCCCGGCTCGCTGCATATCAGACGCCAGCTGTCTCTTTTAAACCGGGCCAGCATTTC
GACACTTCATTCCTTTTGCCTGCAAGTGCTGAAGAAATATTACTACTTGATCGATCTTGATCCGGGCTTTCGGATTGCTG
ATCAGACAGAAGGCGAGCTGCTCGGAGATGAAGTGCTGGACGAGCTGTTTGAAGACGAATACGCAAAGGGCGAGAAGGCG
TTTTTTGAGCTTGTTGACCGCTACACGACAGACCGGCACGATTTGGATCTGCAATTTCTCGTCAAACAGGTGTACGAGTA
TTCCCGATCTCACCCAAACCCGGAGGCATGGCTGGAAAGCTTTGTTCATTTATATGATGTATCGGAAAAGAGCTCAATCG
AGGAGCTTCCATTTTATCAATATGTCAAAGAGGATATTGCGATGGTGCTTAACGGAGCGAAGGAGAAGCTGCAGCGCGCG
CTTGAGCTGACGAAAGCGCCGGGCGGCCCCGCGCCGCGCGCTGACAATTTTTTTGATGATCTTGCGCAGATTGATGAACT
GATTCAGCATCAGGACGATTTCACTGAGCTTTATAAGCGGGTGCCCGCAGTCTCTTTTAAGCGCGCGAAAGCAGTAAAAG
GTGACGAGTTTGATCCTGCGCTCCTTGAAGAAGCGACGGATTTGAGAAATGGCGCAAAAAAACTTCTCGAAAAGCTCAAA
ACCGACTACTTTACGAGAAGCCCTGAACAGCATTTGAAAATCTTAGCCGACATGAAGCCTGTCATTGAAACACTCGTACA
GCTTGTCATCAGCTACGGAAAACGATTCGAAGCCGCGAAGCAGGAAAAATCAATCATTGATTTTTCAGATTTGGAGCATT
ACTGTTTATCCATTTTGACAGCTGAGAACGACAAAGGCGAACGGGAGCCGAGCGAGGCAGCAAGATTTTATCAAGAGCAG
TTTCATGAGGTGCTCGTTGACGAATATCAGGATACCAACCTAGTGCAGGAATCGATCCTGCAGCTCGTCACAAGCGGACC
GGAAGAGACCGGCAACCTGTTTATGGTAGGAGATGTCAAACAGTCGATTTATCGATTCAGGCTTGCGGAGCCGCTTCTTT
TCCTCTCCAAATACAAACGGTTTACAGAGAGCGGAGAAGGCACGGGACGGAAAATCGACTTGAATAAGAATTTCCGAAGC
CGGGCTGATATTTTAGATAGCACAAACTTTTTATTTAAACAGCTGATGGGCGGCAAAATCGGTGAGGTTGATTATGACGA
GCAGGCTGAGCTGAAGCTTGGTGCGGCGTACCCGGACAATGATGAGACGGAAACAGAGCTGCTGCTGATCGACAACTCAG
AAGAGACGGATGCAGGCGAGGAAGCAGAAGAGCTGGAAACGGTGCAGTTCGAGGCGAAAGCGATCGCTAAGGAAATTCGT
AAGTTGATTTCATCGCCATTTAAGGTGTATGACGGAAAAACGAAAACACACCGCAATATTCAATACCGCGATATCGTGAT
ATTGTTACGTTCCATGCCGTGGGCGCCGCAAATCATGGAGGAGCTGAGAGCACAGGGCATACCGGTTTACGCCAATTTAA
TGTCAGGCTATTTTGAAGCGGTCGAAGTCGCCGTCGCGCTTTCTGTGCTAAAGGTGATCGATAACCCGTATCAGGATATA
CCGCTTGCCTCTGTTCTGCGCTCACCGATTGTCGGAGCAGATGAAAACGAGCTGTCTTTGATCCGGCTTGAAAATACAAA
AGCGCCGTACTATGAAGCGATGAAAGACTACTTGGCTGCCGGCGACCGGAACGACGAGCTTTATCAAAAGCTGCAAACGT
TCTATGGACATCTGCAAAAATGGCGTGCGTTTTCGAAAAACCACTCAGTATCTGAGCTGATTTGGGAAGTGTACCGCGAT
ACAAAATACATGGATTATGTCGGCGGGATGCCGGGCGGAAAACAGCGCCAGGCCAATTTGCGTGTTCTCTACGACCGGGC
GCGCCAATATGAATCGACGGCATTTCGCGGCCTGTTCCGTTTCCTGCGGTTTATCGAACGCATGCAGGAGCGGGGCGATG
ATCTTGGAACAGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAA
TTTCCAGTCGTATTTGCAGCGGGTCTCGGACGGAACTTCAACATGATGGACTTGAACAAATCGTACCTGCTGGACAAGGA
GCTCGGGTTTGGCACGAAGTTTATTCATCCGCAATTGCGCATCAGCTATCCGACGCTTCCGCTCATTGCCATGAAGAAAA
AAATGCGCAGGGAGCTATTGTCAGAGGAATTGCGTGTTCTCTATGTCGCATTAACGAGAGCGAAGGAAAAGCTGTTTCTG
ATTGGCTCGTGCAAGGATCATCAGAAACAGCTTGCAAAATGGCAGGCAACTGCGTCCCAAACTGACTGGCTTCTCCCGGA
ATTTGACCGCTATCAGGCAAAAACCTATTTAGATTTCATCGGGCCGGCTCTTGCCAGACACAGAGATTTGGAGGCTTTGG
CTGAAAACGGTGCGGCAGCGCATGCTGACATATCAGGCCATCCGGCGCGTTTTGCCGTTCAAATGGTCCATTCCTATGAT
TTGCTGGATGATGATCTGGAAGAAAGATTGGAAGAAAAAAGCGAGCGCCTAGAAGCGATCCGCCGAGGTGAACCAGTTCC
CGGCTCGTTTGCGTTTGATGAGAAAGCCCGGGAGCAGCTAAGCTGGACATATCCGCATCATGAGGTGACGCAAATTCGCA
CAAAGCAGTCAGTTTCTGAGATCAAGAGAAAAAGAGAGTACGAGGATGAATACAGCGGCAGGGCCCCCGTTAAACCGGCA
GACGGAAGCATTCTGTACAGGCGTCCCGCTTTTATGATGAAAAAAGGTTTGACAGCGGCGGAAAAAGGGACGGCCATGCA
TACGGTTATGCAGCATATCCCGCTCACACATGTGCCGACAATAGAAGAAGCGAAGCAGACGGTTCACAAGCTTTATGAAA
AAGAGCTGCTCACTGAAGAACAAAAAGCCGCTATTGATATAGAAGAAATCGTTCAATTTTTCCATACAGAAATCGGCGGA
CAGCTGATCGGCGCTAAATGGAAGGACCGGGAAGTGCCATTCAGTTTAGCGCTTCCGGCTAAGGAGATCTATCCTGATGC
ACAGGAGGCGGATGAGCCGCTTTTAGTGCAGGGAATCATCGACTGTCTCTATGAAACTGAGGACGGTTTATATCTCTTGG
ACTATAAATCAGACCGGATTGAGGGCAAATTCCAGCACGGCTTTGAAGGAGCGGCCCCGATCTTGAAAAAACGATACGAA
ACGCAAATTCAGCTGTACACGAAGGCAGTTGAGCAAATTGCAAAAACACAGGTAAAGGGATGTGCGCTTTATTTCTTTGA
CGGAGGGCACATTCTGACACTATAG

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1225)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-882)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

96.677

100

0.967