Detailed information NTG0887999

Bioinformatically predicted

Overview

• Name: addB
• Type: Machinery gene
• Locus tag: RS399_RS04880
• Genome accession: NZ_CP135973
• Coordinates: 946150..949650 (-)
• Length: 1166 a.a.
• NCBI ID: WP_084991642.1
• Uniprot ID: -
• Organism: Bacillus inaquosorum strain SI3
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 941150..954650

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RS399_RS04870 (RS399_04870)	sbcD	941212..942387 (-)	1176	WP_084991638.1	exonuclease subunit SbcD	-
RS399_RS04875 (RS399_04875)	addA	942459..946163 (-)	3705	WP_084991639.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
RS399_RS04880 (RS399_04880)	addB	946150..949650 (-)	3501	WP_084991642.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
RS399_RS04885 (RS399_04885)	-	949833..950270 (+)	438	WP_084991645.1	ferritin family protein	-
RS399_RS04890 (RS399_04890)	-	950389..950718 (+)	330	WP_019257992.1	four-helix bundle copper-binding protein	-
RS399_RS04895 (RS399_04895)	-	950753..952480 (-)	1728	WP_084991647.1	ABC transporter substrate-binding protein	-
RS399_RS04900 (RS399_04900)	-	952594..953796 (+)	1203	WP_084991649.1	MFS transporter	-

Sequence

Protein

• Length: 1166 a.a.
• Molecular weight: 134545.63 Da
• Isoelectric Point: 5.6336

>NTDB_id=887999 RS399_RS04880 WP_084991642.1 946150..949650(-) (addB) [Bacillus inaquosorum strain SI3]
MGAEFLVGRSGSGKTKLIIDSIQDELRRAPFGKPIIFLVPDQMTFLMEYELAKTPDMGGMIRAQVFSFSRLAWRFLQHTG
GMSRPFLTSTGVQMLLRKLIEEHKQEFKVYQKASDKSGFTAQVERMLTEFKRYCLEPEDIRRMAESGTASEYRGERILSE
KLHDLSILYQQMEKSLAGQYLHSEDYLTLLAEHIPLAEDVKGAHIYVDGFYQFTPQEFRVLEQLMVHAEHITFSLTADKP
SYEREPHELELFRMTGKTYYRLHQKAKELNLGITYKELSGTERHTSTLELAHLEAQYEARPAVPYAEKQEAFTVMQAANR
RAELEGIAREIHALVREKGYRYKDVAILARQPEDYKDMVKEVFADYEIPYFIDGKASMLNHPLIEFIRSSLDVLKGNWRY
EAVFRCVKTELLFPLNEPKTKVREQVDQLENYCIAYGIKGDRWTKGDRFHYRRFVSLDDDFAQTDQEIEMENMLNDTRDW
IVPPLFQLQKRMKKAKTVQEKAEALYRYLEETDVPLKLDQERQRAEEDGRIIEAQQHQQAWDAVIQLLEEFVEMMGDDEI
SLDLFQQMMEAGAESLTFSLIPPALDQVFVGNMDLSRMYGTSCTFVLGANDGVLPARPDENGVLSDDDREWLKTVGVELS
SGGRERLLDEHFLIYMAFSSPSDRLYVSYPIADAEGKTLLPSIVIKRLEELFPQHKERLLTNEPEQVSDEVQLMYVVNKS
VAQSFTASQLRLWTREYDISDVWWSTYNVLMSEPDRLQSKKLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKMDWRDLTKEQCELFSYDAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGKGPLPPLTFKLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMRATPAGVLYFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGL
LLGDQEAVRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEKEFELLTKHVRRTFQEAGEQITDGRVSIEPYKMKNKTP
CTYCAFKSVCQFDESLEENEYRPLKAEKDKTILEWIKKEADGNEHS

Nucleotide

• Length: 3501 bp

>NTDB_id=887999 RS399_RS04880 WP_084991642.1 946150..949650(-) (addB) [Bacillus inaquosorum strain SI3]
TTGGGAGCAGAGTTTTTAGTAGGCAGGTCCGGGAGTGGAAAAACGAAGCTGATCATCGACAGCATTCAGGATGAACTGCG
CCGGGCTCCATTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTGCTAAAA
CGCCTGATATGGGCGGAATGATACGCGCTCAAGTGTTCAGCTTTTCACGATTGGCCTGGCGGTTCCTCCAGCATACGGGA
GGCATGAGCAGGCCGTTTCTTACTAGCACAGGCGTACAGATGCTCCTGCGGAAGCTCATTGAGGAGCATAAACAGGAGTT
TAAAGTCTATCAAAAAGCGAGTGACAAAAGCGGGTTTACCGCACAGGTAGAACGGATGCTGACAGAGTTTAAGCGCTACT
GTCTGGAACCGGAAGACATCCGCCGGATGGCGGAAAGCGGAACGGCTTCTGAGTATCGCGGAGAACGTATTTTATCTGAG
AAGCTTCATGATTTATCGATTTTATATCAACAGATGGAAAAAAGCCTTGCAGGTCAATACCTTCACTCTGAGGATTATTT
AACATTGCTGGCAGAGCATATTCCGCTTGCGGAAGATGTAAAAGGCGCGCATATCTATGTGGACGGCTTTTATCAGTTTA
CACCGCAGGAATTCAGGGTGTTGGAACAGCTTATGGTTCATGCAGAGCACATCACGTTTTCGCTGACAGCGGACAAGCCG
TCATATGAGCGGGAGCCGCATGAACTGGAATTGTTCAGAATGACTGGAAAAACCTATTACCGCCTGCACCAAAAGGCGAA
GGAACTGAATCTGGGCATTACGTATAAAGAGCTAAGCGGGACTGAACGGCATACGAGCACACTGGAATTGGCACATTTAG
AGGCGCAGTATGAAGCGCGTCCAGCCGTTCCGTACGCAGAAAAACAAGAGGCCTTTACTGTGATGCAGGCCGCAAACAGA
CGAGCTGAGCTGGAAGGCATTGCCCGGGAAATTCACGCTCTTGTCAGAGAGAAGGGCTATCGCTATAAGGATGTAGCGAT
TCTTGCGCGCCAGCCGGAAGACTACAAGGATATGGTGAAGGAAGTTTTCGCAGATTACGAGATTCCTTATTTCATTGACG
GAAAAGCATCAATGCTGAACCATCCGTTAATCGAATTTATCCGGTCGAGCCTTGATGTTTTGAAAGGGAATTGGCGTTAT
GAAGCAGTGTTTCGCTGCGTGAAAACCGAACTGCTATTCCCGCTTAACGAGCCGAAGACTAAGGTGAGAGAACAGGTCGA
TCAGCTTGAAAATTACTGTATCGCCTACGGGATCAAAGGTGACCGCTGGACAAAGGGCGACCGTTTCCATTACAGACGTT
TTGTGTCATTGGATGATGATTTTGCGCAGACCGACCAGGAAATCGAAATGGAAAACATGTTGAATGATACACGCGACTGG
ATTGTTCCTCCGCTTTTTCAGCTCCAGAAACGAATGAAAAAAGCAAAGACGGTTCAGGAGAAGGCGGAGGCGCTCTACCG
CTATCTAGAAGAGACGGATGTGCCGCTGAAGCTGGATCAGGAAAGACAGCGTGCAGAGGAAGACGGCAGAATCATTGAAG
CACAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTGCTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATT
TCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCAGAGTCGCTTACATTTTCTCTTATTCCGCCTGCGCTTGACCA
GGTGTTTGTCGGCAATATGGATTTGTCCAGAATGTATGGCACCTCCTGTACCTTTGTGCTGGGGGCAAACGACGGCGTTC
TGCCGGCGCGCCCAGATGAAAACGGAGTGCTGTCGGATGATGACCGGGAATGGCTGAAAACGGTTGGGGTTGAGCTTTCC
TCAGGCGGGCGGGAGCGTCTGCTTGATGAGCATTTCCTCATCTATATGGCGTTTTCCAGTCCGTCTGACCGGCTGTACGT
ATCGTATCCGATTGCTGATGCGGAAGGAAAAACGCTTCTGCCGTCGATTGTCATTAAGCGGCTGGAAGAACTGTTTCCGC
AGCATAAGGAGCGCCTGTTGACAAATGAACCTGAGCAGGTCAGCGATGAGGTACAGCTGATGTATGTCGTCAATAAAAGT
GTGGCACAGTCCTTTACCGCCAGCCAGCTCAGATTATGGACTCGGGAATACGACATCAGCGACGTCTGGTGGAGCACGTA
CAATGTGCTGATGAGTGAGCCGGACAGGCTGCAATCGAAAAAGCTATTCTCAAGCCTGTTTTTCCGGAATGAAGTGAAGC
AGCTTGAACGCAGCGTGTCGAGACAGCTCTATGGTGAACGCATTCAGGGCAGTGTATCGAGAATGGAAACCTTTAACGCG
TGCCCGTTCTCCCATTTTGCGTCACACGGGCTGCATCTGAAGGAACGGCAATTTTTCAAGCTCGAAGCACCGGATATCGG
CCAGCTGTTTCATTCCAGCTTAAAGCTGATTTCAGACCGGCTGCGCGAGCAAAAAATGGATTGGCGCGATTTAACGAAAG
AGCAGTGCGAGCTGTTCTCCTATGATGCGGTAGAGCGGCTGGCGCCGAAACTGCAAAAGGAAATTTTGCTCAGCTCAAAC
CGGCATTATTATGTGAAGGAAAAACTGCAAAAAATTGTGACACGCGTGTCCGGCATTTTAAGCGAGCATGCGAAAGCGAG
CGGATTCGTGCCCATCGGGCTTGAACTGGGCTTTGGAGGAAAAGGGCCGCTTCCGCCGCTGACCTTTAAGCTGAAAAACG
GCTGCACAATGGAGCTCGTCGGGCGAATTGACCGCGTCGATAAAGCGGAAAGCTCCAAAGGCCTGCTTCTAAGGATTGTC
GATTATAAATCAAGCGACAAAGGCCTTGACTTAGCGGAAGTATATTATGGACTGGCGCTGCAAATGCTGACGTACCTTGA
TTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGAGCGACGCCGGCCGGAGTGCTGTATTTCCATATTCATGACC
CAATGATTCAATCTAACCTCCCGCTTGGGCTTGACGAGATCGAACAGGAGATCTTTAAGAAATTTAAGATGAAGGGCCTG
CTCCTCGGCGATCAGGAAGCGGTTCGCCTCATGGATACAACCCTTCAAGAGGGACGATCAAATATCATCAACGCCGGCTT
GAAGAAAGACGGCTCTCTCAGATCGGATTCAGCGGCAGTCGGCGAAAAGGAATTTGAACTGCTGACGAAGCATGTGCGCC
GCACCTTCCAAGAAGCAGGCGAACAAATCACCGACGGACGCGTATCGATTGAGCCTTATAAAATGAAGAACAAGACGCCG
TGCACATACTGTGCGTTCAAATCAGTATGCCAATTTGATGAATCATTAGAAGAAAACGAGTATCGCCCGTTAAAGGCTGA
AAAGGACAAGACGATACTTGAGTGGATAAAAAAGGAGGCGGATGGCAATGAACATTCCTAA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (306-668)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (790-1131)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addB	Bacillus subtilis subsp. subtilis str. 168	97.856	100	0.979