Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   RUL31_RS13585 Genome accession   NZ_CP135964
Coordinates   2782914..2783537 (-) Length   207 a.a.
NCBI ID   WP_106270954.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain CD303     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2777914..2788537
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RUL31_RS13555 (RUL31_13555) rpsT 2778208..2778474 (+) 267 WP_003325396.1 30S ribosomal protein S20 -
  RUL31_RS13560 (RUL31_13560) holA 2778489..2779532 (-) 1044 WP_106360516.1 DNA polymerase III subunit delta -
  RUL31_RS13565 (RUL31_13565) - 2779572..2779721 (-) 150 WP_181279838.1 hypothetical protein -
  RUL31_RS13570 (RUL31_13570) - 2779762..2779896 (+) 135 WP_064505211.1 YqzM family protein -
  RUL31_RS13575 (RUL31_13575) comEC 2779937..2782270 (-) 2334 WP_088117605.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  RUL31_RS13580 (RUL31_13580) - 2782277..2782846 (-) 570 WP_003325392.1 ComE operon protein 2 -
  RUL31_RS13585 (RUL31_13585) comEA 2782914..2783537 (-) 624 WP_106270954.1 helix-hairpin-helix domain-containing protein Machinery gene
  RUL31_RS13590 (RUL31_13590) comER 2783621..2784439 (+) 819 WP_315915426.1 late competence protein ComER -
  RUL31_RS13595 (RUL31_13595) - 2784509..2785249 (-) 741 WP_315915427.1 class I SAM-dependent methyltransferase -
  RUL31_RS13600 (RUL31_13600) rsfS 2785246..2785602 (-) 357 WP_003325387.1 ribosome silencing factor -
  RUL31_RS13605 (RUL31_13605) yqeK 2785619..2786179 (-) 561 WP_010789259.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  RUL31_RS13610 (RUL31_13610) - 2786169..2786738 (-) 570 WP_315915429.1 nicotinate-nucleotide adenylyltransferase -
  RUL31_RS13615 (RUL31_13615) yhbY 2786750..2787040 (-) 291 WP_003325384.1 ribosome assembly RNA-binding protein YhbY -
  RUL31_RS13620 (RUL31_13620) aroE 2787034..2787876 (-) 843 WP_315915430.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 21962.75 Da        Isoelectric Point: 4.6181

>NTDB_id=887835 RUL31_RS13585 WP_106270954.1 2782914..2783537(-) (comEA) [Bacillus atrophaeus strain CD303]
MNWFYQHKKIILTAGAAVIFIVILLNFPGAKKQEPVQQITASPDTAGTQVQQQESKGDADGEKIIIDIKGAVKHPGVYEL
KTGDRVSQAIEKAGGINSEADEKQVNLAELLQDGTVVYIPSEGEEPTQSAAATASVQNGAGKEALININTASLEELQAIS
GVGPSKAEAIIAYREENGKFQAVEDITNVSGIGEKSFEKIKSAITVK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=887835 RUL31_RS13585 WP_106270954.1 2782914..2783537(-) (comEA) [Bacillus atrophaeus strain CD303]
ATGAATTGGTTTTACCAGCACAAAAAAATAATCTTAACGGCAGGTGCTGCTGTCATCTTCATCGTCATCCTGCTTAATTT
TCCCGGCGCAAAGAAGCAGGAGCCTGTTCAGCAAATCACAGCGTCGCCTGACACGGCGGGCACACAAGTTCAGCAGCAAG
AATCGAAAGGTGATGCTGACGGAGAAAAAATTATCATTGATATTAAAGGCGCTGTCAAACATCCAGGTGTTTACGAATTG
AAAACGGGAGACAGAGTTTCACAGGCTATCGAAAAAGCGGGAGGCATTAATTCGGAGGCTGATGAAAAACAAGTAAATTT
AGCTGAACTCCTGCAGGACGGCACTGTCGTGTACATTCCGAGTGAGGGGGAAGAGCCAACACAAAGCGCTGCCGCAACTG
CTTCTGTGCAAAACGGAGCAGGAAAAGAGGCTTTGATTAATATCAATACAGCGTCATTAGAGGAACTGCAGGCTATTTCC
GGGGTCGGTCCGTCAAAAGCAGAAGCTATAATAGCTTATCGTGAAGAAAACGGAAAATTTCAAGCCGTTGAAGACATTAC
AAATGTCTCGGGAATCGGCGAAAAATCATTTGAGAAAATAAAATCAGCCATCACAGTTAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(66-120)

Helix-hairpin-helix motif (HHH_3)

(143-205)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

65.217

100

0.652