Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   RUL31_RS13145 Genome accession   NZ_CP135964
Coordinates   2701757..2702827 (-) Length   356 a.a.
NCBI ID   WP_106270996.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain CD303     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2696757..2707827
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RUL31_RS13100 (RUL31_13100) tapA 2697292..2697993 (-) 702 WP_315915357.1 amyloid fiber anchoring/assembly protein TapA -
  RUL31_RS13105 (RUL31_13105) - 2698255..2698578 (+) 324 WP_088117568.1 DUF3889 domain-containing protein -
  RUL31_RS13110 (RUL31_13110) - 2698625..2698804 (-) 180 WP_003325435.1 YqzE family protein -
  RUL31_RS13115 (RUL31_13115) comGG 2698874..2699248 (-) 375 WP_088117569.1 competence type IV pilus minor pilin ComGG Machinery gene
  RUL31_RS13120 (RUL31_13120) comGF 2699249..2699644 (-) 396 WP_309484978.1 competence type IV pilus minor pilin ComGF Machinery gene
  RUL31_RS13125 (RUL31_13125) comGE 2699658..2700005 (-) 348 WP_088117570.1 competence type IV pilus minor pilin ComGE Machinery gene
  RUL31_RS13130 (RUL31_13130) comGD 2699989..2700429 (-) 441 WP_390840628.1 competence type IV pilus minor pilin ComGD Machinery gene
  RUL31_RS13135 (RUL31_13135) comGC 2700419..2700718 (-) 300 WP_003325429.1 competence type IV pilus major pilin ComGC Machinery gene
  RUL31_RS13140 (RUL31_13140) comGB 2700733..2701770 (-) 1038 WP_315915363.1 competence type IV pilus assembly protein ComGB Machinery gene
  RUL31_RS13145 (RUL31_13145) comGA 2701757..2702827 (-) 1071 WP_106270996.1 competence type IV pilus ATPase ComGA Machinery gene
  RUL31_RS13150 (RUL31_13150) - 2703207..2704160 (-) 954 WP_088117575.1 magnesium transporter CorA family protein -
  RUL31_RS13155 (RUL31_13155) - 2704282..2705630 (+) 1349 Protein_2562 hemolysin family protein -
  RUL31_RS13160 (RUL31_13160) - 2705697..2706548 (-) 852 WP_199680830.1 STAS domain-containing protein -
  RUL31_RS13170 (RUL31_13170) - 2706815..2707195 (-) 381 WP_003325422.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  RUL31_RS13175 (RUL31_13175) - 2707433..2707678 (+) 246 WP_003325421.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40511.76 Da        Isoelectric Point: 9.1917

>NTDB_id=887831 RUL31_RS13145 WP_106270996.1 2701757..2702827(-) (comGA) [Bacillus atrophaeus strain CD303]
MDPIEKTSRTLIEEAYSVKASDIHIVPREQDALIHFRIDHALLKKRTMKKEECVRLISHFKFLSAMDIGERRKPQSGSLS
LKLKNETVYLRMSTLPTINDESLVIRVLPQHNIPAIERLSLFPKTGATLLSFLKHSHGLIIFTGPTGSGKSTTLYSLVQY
AKKHFNRNIITLEDPVETRDQDVLQVQVNEKAGITYSAGLKAILRHDPDMIVLGEIRDAETAKIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGVNMNEIEQTAIAIAAQRLVDLTCPFCENGCSSVYCRITRDVRRATIYELLYGKNLQQCIQEARGE
RANYQYRTLRQIIRKGIALGYLTTNNYDRWVYHEED

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=887831 RUL31_RS13145 WP_106270996.1 2701757..2702827(-) (comGA) [Bacillus atrophaeus strain CD303]
TTGGATCCGATAGAAAAAACTAGCAGGACATTAATTGAAGAGGCATATTCCGTGAAGGCCTCGGATATTCACATTGTACC
AAGGGAGCAAGATGCTTTGATCCATTTTCGAATTGACCATGCATTGTTGAAGAAGCGGACAATGAAAAAGGAAGAGTGCG
TTCGGCTCATTTCCCATTTTAAATTTCTGTCGGCCATGGATATAGGAGAAAGGCGGAAGCCGCAAAGTGGTTCACTTTCT
TTAAAGCTTAAAAATGAGACAGTTTATCTCAGAATGTCGACACTTCCTACGATTAATGATGAAAGCCTTGTGATCAGAGT
TTTGCCTCAGCATAATATTCCTGCGATCGAAAGACTGTCCCTGTTCCCAAAGACAGGCGCCACGCTGCTATCCTTTTTAA
AGCACTCCCATGGCCTCATTATTTTTACCGGTCCGACAGGCTCAGGGAAATCTACCACCCTATATTCACTGGTTCAATAT
GCAAAAAAGCATTTCAACCGAAATATTATCACTCTCGAAGATCCTGTGGAAACTAGAGATCAGGATGTCTTGCAGGTACA
GGTGAATGAAAAAGCCGGCATTACGTACTCAGCGGGCCTGAAGGCGATTTTGCGCCATGATCCAGACATGATTGTTCTGG
GGGAAATCAGGGATGCTGAAACAGCAAAAATCGCCGTGCGGGCTGCTATGACAGGTCATTTGGTATTAACAAGCCTGCAC
ACAAGAGATGCCAAGGGCGCCATTTACAGACTGCTTGAATTCGGAGTAAATATGAATGAAATAGAGCAAACAGCGATTGC
GATAGCAGCCCAGCGATTAGTCGACTTAACCTGTCCATTTTGTGAAAACGGCTGTTCCTCTGTTTATTGCCGGATTACAC
GTGATGTAAGGCGAGCGACCATTTATGAGCTTCTTTACGGGAAAAATCTTCAGCAATGTATACAAGAAGCTAGAGGAGAA
CGGGCCAATTATCAGTACCGAACCTTACGGCAGATTATCAGAAAAGGAATCGCGCTTGGCTATCTAACGACGAATAATTA
CGACCGGTGGGTTTATCATGAAGAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

87.64

100

0.876
  pilB Glaesserella parasuis strain SC1401

44.027

82.303

0.362