Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   RUL31_RS06665 Genome accession   NZ_CP135964
Coordinates   1351875..1352300 (+) Length   141 a.a.
NCBI ID   WP_081250945.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain CD303     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1346875..1357300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RUL31_RS06635 (RUL31_06635) - 1347336..1347529 (+) 194 Protein_1258 peptidoglycan-binding protein -
  RUL31_RS06640 (RUL31_06640) - 1348245..1348583 (-) 339 WP_315916755.1 contact-dependent growth inhibition system immunity protein -
  RUL31_RS06645 (RUL31_06645) - 1348572..1348892 (+) 321 WP_315916756.1 putative quinol monooxygenase -
  RUL31_RS06650 (RUL31_06650) - 1350065..1350346 (-) 282 WP_315916757.1 LCI family antimicrobial peptide -
  RUL31_RS21315 - 1350740..1350880 (+) 141 Protein_1262 hypothetical protein -
  RUL31_RS06655 (RUL31_06655) - 1351017..1351346 (-) 330 WP_315916758.1 hypothetical protein -
  RUL31_RS06660 (RUL31_06660) - 1351406..1351684 (-) 279 WP_003327515.1 hypothetical protein -
  RUL31_RS06665 (RUL31_06665) nucA/comI 1351875..1352300 (+) 426 WP_081250945.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  RUL31_RS06670 (RUL31_06670) - 1352449..1353111 (+) 663 WP_010788290.1 MgtC/SapB family protein -
  RUL31_RS06675 (RUL31_06675) - 1353170..1353409 (-) 240 WP_106359748.1 hypothetical protein -
  RUL31_RS06680 (RUL31_06680) - 1353622..1353765 (-) 144 WP_223251816.1 aspartyl-phosphate phosphatase Spo0E family protein -
  RUL31_RS06685 (RUL31_06685) - 1353894..1354109 (-) 216 WP_081112782.1 spore coat protein -
  RUL31_RS06690 (RUL31_06690) - 1354424..1355824 (+) 1401 WP_315916759.1 polysaccharide deacetylase family protein -
  RUL31_RS06695 (RUL31_06695) - 1355877..1356350 (-) 474 WP_315916760.1 YjfA family protein -
  RUL31_RS06700 (RUL31_06700) - 1356487..1356897 (-) 411 WP_307441944.1 DUF2809 domain-containing protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15458.32 Da        Isoelectric Point: 4.7462

>NTDB_id=887809 RUL31_RS06665 WP_081250945.1 1351875..1352300(+) (nucA/comI) [Bacillus atrophaeus strain CD303]
MKKWITSLFLTAAILFGLMGAEVYAPQDVKGASRYDQVLYFPFSRYPETGDHIKDAIAAGHSDVCTIDRAGADERREESL
KGIPTKPGYDRDEWPMAVCEEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 426 bp        

>NTDB_id=887809 RUL31_RS06665 WP_081250945.1 1351875..1352300(+) (nucA/comI) [Bacillus atrophaeus strain CD303]
ATGAAAAAATGGATCACGAGCTTATTTCTGACCGCAGCGATATTGTTTGGATTGATGGGTGCGGAAGTGTATGCTCCGCA
GGATGTCAAAGGCGCATCACGGTATGATCAGGTCCTTTACTTCCCATTTTCACGTTATCCTGAAACCGGCGATCACATTA
AAGATGCGATAGCGGCCGGGCATTCAGATGTATGTACGATTGACAGGGCCGGGGCGGATGAGCGCAGGGAGGAGTCTTTA
AAGGGGATTCCTACGAAACCGGGGTATGACCGGGATGAGTGGCCGATGGCTGTATGTGAAGAAGGCGGGGCCGGGGCGGA
TATCCGGTATGTCACCCCATCTGATAATCGAGGCGCCGGTTCATGGGTCGGGAATCAAATGAGCGGTTATTCTGATGGTA
CGAGAGTATTATTTATCGTACAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(45-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

59.524

89.362

0.532