Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   RRV45_RS07460 Genome accession   NZ_CP135435
Coordinates   1503995..1507735 (+) Length   1246 a.a.
NCBI ID   WP_315668187.1    Uniprot ID   A0AA96MY98
Organism   Bacillus sp. DTU_2020_1000418_1_SI_GHA_SEK_038     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1498995..1512735
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RRV45_RS07455 (RRV45_07455) addB 1500508..1504017 (+) 3510 WP_315668186.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  RRV45_RS07460 (RRV45_07460) addA 1503995..1507735 (+) 3741 WP_315668187.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  RRV45_RS07465 (RRV45_07465) - 1507779..1507997 (-) 219 WP_315668188.1 spore germination protein -
  RRV45_RS07470 (RRV45_07470) - 1508043..1508414 (-) 372 WP_315668189.1 spore germination protein GerPE -
  RRV45_RS07475 (RRV45_07475) - 1508421..1508597 (-) 177 WP_315668190.1 spore gernimation protein GerPD -
  RRV45_RS07480 (RRV45_07480) gerPC 1508587..1509216 (-) 630 WP_315668191.1 spore germination protein GerPC -
  RRV45_RS07485 (RRV45_07485) - 1509257..1509493 (-) 237 WP_315668193.1 spore germination protein GerPB -
  RRV45_RS07490 (RRV45_07490) - 1509509..1509730 (-) 222 WP_315668194.1 spore germination protein -
  RRV45_RS07495 (RRV45_07495) - 1509907..1510260 (+) 354 WP_315668195.1 YisL family protein -

Sequence

Protein


Download         Length: 1246 a.a.        Molecular weight: 143596.13 Da        Isoelectric Point: 4.8757

>NTDB_id=886462 RRV45_RS07460 WP_315668187.1 1503995..1507735(+) (addA) [Bacillus sp. DTU_2020_1000418_1_SI_GHA_SEK_038]
MKSTIPAKPANVTWTDDQWKAIMAKGQDILVAAAAGSGKTAVLVERIINKIVSEEESINVDELLVVTFTNASAAEMRHRI
GEALDKAINANPKSAHLRKQLSLLNKASISTLHSFCLEMVRKYYYLLDIDPSFRIADETEAQLLRDEVLEELFEEEYGKE
GNDSFFALVDTFTNDRSDTALQDIIRDIYDFARSNPSPDEYLDSIIEMYNVDENSEIEGMPFVQSLLQDMDLQLKGAKQL
LEYGLELTKLPGGPAPRAENFIADLHVVQTLLEAKKDSWQTLYEAMQSWSFGRAKNCKGDEYDSELIEKAKKLRDKAKKI
IQDLQDELFSRKPDSFLRDMKEMKQYIETLVYIVKEFSIRFNQIKTEKGLVDFADLEHFCLEILSDKSDEGNLTPSDAAV
ACRNQFKEVLVDEYQDVNMVQEAILQLVTMDSEVAGNLFMVGDVKQSIYRFRLAEPNLFLGKYNRFEKNGEEAGLRIDLA
KNFRSRKEVLHGTNYIFKQIMGVSVGEIEYDEAAELKAGAPYPEDESYPIELTVIDLEGTEEEEPQDNEQDGFDAAELAQ
SQLEARFMAAKIKEIISERKNVYNPKMKSGRPAEYRDIVILLRSMTWAPQIMEEFKQQGIPVYANLSTGYFEATEVSIMM
SLLKIIDNPYQDIPLAAVLRSPIVGLTEEELSQIRIQHKKASFYEAMASFCNVKPAFELEHLYAKVRPFYEQLMNWRVMA
RQGSLSELIWQLYRETRFYDFVGGMPGGKQRQANLRAFFDRARQYEATSFRGLFRFLRFIERMRDRGDDLGAARALGEQE
DVVRLMTIHSSKGLEFPFVFIAGLGRNFNTMDLKKAYMLDKEYGFAAKYVNAEKRISYPSLPQIAFKRKKKMEMLSEEMR
VLYVALTRAKEKLYLVSSVKKAEKKVNQWLQSAENPNWLLNEFDRASAISFLDWIGPALVRHRDYDSRISQLISSEITDH
PSTWKVQIINSEEVAKLVEVLEAEKDTYLQMVFSGEEVPVQSPFKTMVEDQLSWKYSYLEASQNRSKQSVSEVKRQKEIF
SQEDSGIEIIRQFKKPILNRPQFMQEKSLSPAERGTAMHMVMQHISFKEQMTVKSISAKMDEMVRKELLTEEQRAAIDPS
LIELFFKTELGRRMIMAREINREIPFSVSLPASDIYPEWKGEEESVLIQGIIDCLFEDEQGFVLIDYKTDQITGRFKGGI
DEAKSVLANRYRVQIQMYTRALEQILKRDIQERYLYFFDGALIIKL

Nucleotide


Download         Length: 3741 bp        

>NTDB_id=886462 RRV45_RS07460 WP_315668187.1 1503995..1507735(+) (addA) [Bacillus sp. DTU_2020_1000418_1_SI_GHA_SEK_038]
ATGAAATCAACTATTCCAGCTAAGCCAGCTAATGTAACATGGACAGATGATCAATGGAAGGCGATTATGGCAAAGGGGCA
GGATATTCTTGTTGCAGCAGCGGCAGGTTCCGGGAAAACAGCTGTCCTTGTTGAAAGGATCATTAATAAAATTGTATCAG
AGGAAGAATCAATCAACGTTGATGAGTTGCTTGTTGTGACATTTACGAATGCCTCAGCAGCAGAAATGAGACACAGGATT
GGAGAGGCTCTTGATAAAGCCATTAATGCAAATCCTAAATCTGCCCATTTACGAAAGCAGCTGAGCCTGTTAAATAAAGC
ATCGATCTCTACACTTCACTCATTTTGTCTGGAAATGGTTAGAAAATACTACTATTTACTCGATATTGATCCGAGCTTTC
GAATTGCTGACGAAACAGAAGCACAGCTTTTAAGAGATGAAGTGCTTGAAGAGCTTTTCGAAGAGGAGTATGGGAAAGAA
GGAAATGATTCGTTTTTTGCTCTTGTAGATACGTTTACAAATGACAGGAGTGACACTGCTCTACAGGACATCATCAGGGA
TATATATGATTTTGCGAGATCAAATCCCTCTCCTGATGAATACCTTGATTCCATCATCGAGATGTATAATGTTGATGAAA
ACAGTGAAATCGAAGGAATGCCCTTTGTACAGTCCCTTTTACAGGATATGGATTTGCAGCTTAAGGGGGCAAAACAGCTG
CTTGAATATGGGCTTGAGCTAACTAAGCTTCCGGGTGGCCCTGCACCAAGAGCAGAAAACTTTATTGCCGACCTTCATGT
TGTCCAAACATTACTGGAAGCGAAAAAAGACTCCTGGCAAACGCTTTACGAGGCGATGCAATCCTGGTCATTTGGCAGGG
CGAAAAATTGCAAGGGCGATGAGTATGATAGCGAGCTAATTGAAAAGGCAAAGAAGCTAAGAGATAAGGCAAAAAAAATC
ATTCAGGATCTTCAGGATGAGCTATTTTCCCGTAAGCCTGATAGCTTCCTGCGAGATATGAAGGAAATGAAGCAGTATAT
TGAGACACTCGTCTATATCGTGAAGGAATTTTCAATACGGTTTAATCAAATAAAGACAGAAAAAGGTTTAGTGGATTTTG
CCGACCTCGAACATTTCTGTTTAGAAATTCTTTCTGATAAGAGTGACGAAGGAAACTTAACACCTTCAGATGCAGCAGTT
GCCTGTAGAAATCAGTTCAAGGAAGTTCTAGTTGATGAGTATCAAGATGTTAACATGGTTCAGGAAGCGATCCTTCAGCT
TGTGACAATGGACTCAGAGGTTGCGGGAAACCTTTTTATGGTGGGCGATGTGAAACAATCTATTTACCGCTTCCGTCTTG
CAGAACCGAATTTATTCTTAGGCAAATACAACAGGTTTGAGAAAAACGGTGAAGAAGCAGGCTTAAGAATTGACCTTGCC
AAAAACTTTAGAAGCCGAAAAGAAGTGCTGCACGGAACGAATTATATATTTAAACAGATTATGGGTGTTTCTGTCGGTGA
AATTGAATATGACGAAGCAGCAGAGCTGAAAGCTGGAGCCCCATATCCAGAAGATGAATCTTATCCCATTGAATTAACGG
TTATCGATTTAGAAGGGACAGAAGAGGAAGAACCGCAGGACAATGAGCAGGATGGGTTTGACGCGGCAGAGCTTGCCCAA
TCCCAGCTAGAGGCAAGATTCATGGCAGCAAAAATAAAAGAAATCATTTCTGAGCGGAAGAACGTATACAATCCAAAAAT
GAAATCAGGGCGTCCGGCGGAATACCGGGATATCGTCATTTTGCTGCGATCGATGACATGGGCACCTCAGATCATGGAGG
AATTTAAGCAACAGGGTATTCCCGTATATGCTAATTTATCAACGGGTTATTTCGAGGCCACTGAAGTATCCATTATGATG
TCACTATTAAAAATAATTGACAATCCATATCAGGATATCCCGCTTGCAGCTGTATTAAGGTCCCCAATTGTCGGCTTGAC
TGAGGAAGAGCTGTCCCAGATAAGAATTCAACACAAAAAGGCTTCGTTTTATGAGGCAATGGCATCTTTTTGCAATGTTA
AGCCTGCATTTGAACTTGAACACTTATATGCAAAAGTCCGGCCATTCTATGAACAGCTAATGAATTGGCGCGTCATGGCA
AGGCAAGGCTCTCTTTCTGAGCTAATCTGGCAATTATATAGAGAGACAAGGTTTTATGATTTCGTTGGCGGCATGCCTGG
CGGAAAGCAAAGACAGGCAAATCTTCGGGCATTTTTTGACCGTGCAAGGCAATATGAAGCTACATCGTTTAGAGGATTAT
TCAGGTTCTTAAGATTTATTGAACGCATGAGGGATAGGGGAGATGATCTCGGTGCTGCACGTGCCTTAGGAGAACAGGAG
GATGTTGTTCGCCTGATGACGATTCATAGCAGCAAAGGGCTTGAGTTTCCTTTCGTATTCATAGCTGGGCTTGGCCGGAA
TTTTAATACGATGGATTTGAAAAAAGCCTATATGCTAGATAAGGAATATGGATTCGCTGCAAAATACGTAAATGCTGAAA
AACGAATATCGTATCCATCTCTTCCCCAAATTGCTTTTAAAAGGAAAAAGAAGATGGAAATGCTTTCGGAAGAAATGCGG
GTCCTCTATGTAGCCTTGACGAGGGCTAAGGAAAAGCTTTACTTAGTATCGTCAGTCAAAAAGGCTGAGAAGAAGGTAAA
TCAATGGCTGCAATCTGCTGAAAATCCAAACTGGCTGTTAAATGAATTTGATCGCGCATCTGCGATAAGCTTTCTTGATT
GGATTGGTCCAGCTCTCGTACGGCATAGAGATTATGATAGCAGGATTAGTCAGCTCATTTCCAGTGAAATTACCGACCAC
CCTTCAACATGGAAGGTTCAGATTATAAACAGTGAAGAGGTTGCAAAACTTGTTGAAGTGCTAGAAGCCGAAAAAGATAC
CTATCTGCAAATGGTATTCTCAGGTGAGGAAGTTCCTGTACAGTCTCCATTTAAGACTATGGTTGAAGACCAATTGTCAT
GGAAATATTCATATTTAGAAGCTTCCCAAAACCGTTCTAAGCAGTCAGTTTCTGAGGTGAAAAGGCAAAAGGAGATATTT
TCCCAGGAAGATAGCGGGATAGAAATCATCCGTCAATTCAAAAAGCCTATATTAAACCGGCCACAATTTATGCAGGAGAA
GTCATTATCTCCGGCAGAACGAGGAACCGCTATGCATATGGTCATGCAGCACATTAGCTTTAAAGAACAGATGACTGTAA
AATCTATTTCTGCCAAAATGGATGAAATGGTTCGTAAAGAATTACTGACAGAAGAGCAGCGAGCGGCCATCGATCCGTCT
TTAATTGAGCTTTTTTTCAAAACTGAGCTTGGAAGAAGAATGATAATGGCAAGAGAGATTAATCGAGAAATTCCTTTCAG
TGTATCTTTACCAGCAAGCGATATTTATCCGGAATGGAAGGGTGAAGAGGAGTCTGTCTTAATCCAGGGGATTATTGATT
GCCTTTTTGAGGATGAACAAGGTTTCGTGTTAATTGACTATAAGACCGATCAAATTACCGGCCGTTTTAAAGGCGGGATT
GATGAAGCAAAATCGGTACTTGCAAATCGTTACAGAGTTCAAATTCAGATGTATACAAGGGCTTTAGAGCAAATTCTAAA
AAGAGACATTCAAGAAAGATATTTATACTTTTTTGATGGAGCACTTATTATTAAATTATAG

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(477-897)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-466)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1062-1227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

55.333

100

0.554