Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   RRV45_RS07455 Genome accession   NZ_CP135435
Coordinates   1500508..1504017 (+) Length   1169 a.a.
NCBI ID   WP_315668186.1    Uniprot ID   A0AA96MZL9
Organism   Bacillus sp. DTU_2020_1000418_1_SI_GHA_SEK_038     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1495508..1509017
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RRV45_RS07430 (RRV45_07430) - 1495924..1496100 (+) 177 WP_315668181.1 hypothetical protein -
  RRV45_RS07435 (RRV45_07435) - 1496129..1497136 (+) 1008 WP_315668182.1 3-oxoacyl-ACP synthase -
  RRV45_RS07440 (RRV45_07440) - 1497144..1498040 (+) 897 WP_315668183.1 alpha/beta hydrolase -
  RRV45_RS07445 (RRV45_07445) fabG 1498054..1498794 (+) 741 WP_315668184.1 3-oxoacyl-ACP reductase FabG -
  RRV45_RS07450 (RRV45_07450) - 1498852..1500369 (+) 1518 WP_315668185.1 long-chain fatty acid--CoA ligase -
  RRV45_RS07455 (RRV45_07455) addB 1500508..1504017 (+) 3510 WP_315668186.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  RRV45_RS07460 (RRV45_07460) addA 1503995..1507735 (+) 3741 WP_315668187.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  RRV45_RS07465 (RRV45_07465) - 1507779..1507997 (-) 219 WP_315668188.1 spore germination protein -
  RRV45_RS07470 (RRV45_07470) - 1508043..1508414 (-) 372 WP_315668189.1 spore germination protein GerPE -
  RRV45_RS07475 (RRV45_07475) - 1508421..1508597 (-) 177 WP_315668190.1 spore gernimation protein GerPD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134811.79 Da        Isoelectric Point: 6.5946

>NTDB_id=886461 RRV45_RS07455 WP_315668186.1 1500508..1504017(+) (addB) [Bacillus sp. DTU_2020_1000418_1_SI_GHA_SEK_038]
MSVRLLIGRSGSGKTDYCLNEIRAELKRDPIGNPPLIYLVPEQMTFLSEYKLITTPGLGGMIRSQVYSFTRLAWKILQET
GGISRYHINSVGINMLIRKIIDEKKDSLKLFQRAADKNGFIQQVEQMMTEFKRYCVQPEELSEKQAQLTQEEHVNKALHD
KLHDLELIYRSFEESLIDKYVDSEDYFRLLVEKAGQSEYLQKAEVYIDGFYSFTPQEFMIVEQLMKHCKKVTITLTLDQP
FKKNPPDELHLFRMSGETCQTIYEIAAQQGLQVEEVILNGQKRWGKPSLKHLEEKFESRPAEGYKGETAVHIGHAANRRA
EIEGIARKINRLVRTKGLRYRDIAILSRNGQDYFDIIGTIFHDYQIPYFIDQKRTMLHHPLVELIRSTLEVINGNWRYEP
IFRAVKTELLFPAKLNAHHLREQMDRLENYCLAYGIQGDKWTNKKRWIYRRIRGLEFESFGQTDAEKEIEQELNELRDMI
TGPILRLSRRLKKAHSGRQYCEAVYLYLEELDVPAKLESWKLEEDNKGNLVKSREHEQSWNAIMELLDQYVEMLGEEEVT
LKQFASILEAGLESLRFTLIPPALDGVMAADLDMSRLSDIKAAFIIGMNDGVFPAKFSEEGLLADSDREQLLVNGLKIAP
ASKTRLLDEEFIAYKAFATPSDWLFISYPLANEEGKALLPSSYIKRLTDLLPDCVHHDYLSDPAELPENEQLEYAVNLHT
SLAYLTSQMGLKKRNYPIYDFWWDIYNYFLHHEQWRGQALKVLSSLNYENKTKQLKEEMSKDLYGDIIQASVSRMELFHG
CPFSHFAQHGLKLKERQVFRLEAPDIGDLFHAALKYIAETVMSKKLSWTDLTRKQCEILAQEAVEMLAPKLQNEILLSSN
RHYYIKRKLEQIISRASYVLSEHAKMSGFSPIGLELGFGPKGELPPLTFPLKNGAKMELIGRIDRVDKAEDKDGVFLRVI
DYKSSSKDINVNEVYYGLALQMLTYLDIIVSHSHALIGTEANPAGILYFHVHNPIISTSKMLTIDDIEQEIFKKFKMNGL
ILGEENVIKLMDQTLDNGDSSIISAGFKKDGTLSKRSKIASKQEFEQLRQYVRKMYTKTGNQITEGKVDISPYKMKDKTP
CTFCSFKPVCQFDESLGANEYRMLVPQPKDDVLASIRKEVGKDEINYSS

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=886461 RRV45_RS07455 WP_315668186.1 1500508..1504017(+) (addB) [Bacillus sp. DTU_2020_1000418_1_SI_GHA_SEK_038]
ATGTCCGTTCGTTTATTAATTGGCCGCTCAGGAAGTGGTAAAACAGACTATTGTTTAAATGAAATAAGAGCTGAATTAAA
AAGGGATCCAATTGGGAATCCACCACTTATTTACTTAGTTCCTGAGCAAATGACATTTCTATCAGAATATAAATTAATTA
CAACACCAGGACTTGGCGGGATGATCCGCAGTCAAGTATATTCCTTCACTCGCCTTGCCTGGAAAATACTTCAGGAAACA
GGCGGTATCAGCCGCTATCATATAAACAGTGTCGGAATTAATATGCTCATTCGAAAAATCATTGATGAGAAAAAGGATAG
TCTTAAGCTGTTTCAGCGTGCTGCCGATAAAAATGGGTTTATCCAGCAAGTAGAACAAATGATGACGGAATTCAAAAGAT
ATTGTGTTCAGCCCGAAGAACTTTCAGAGAAACAAGCTCAACTAACACAAGAGGAACATGTAAATAAGGCACTTCACGAT
AAGCTTCACGATCTTGAACTCATTTACAGAAGCTTTGAAGAATCACTGATTGATAAATACGTAGACTCCGAGGATTACTT
TCGGCTCCTAGTGGAAAAAGCAGGACAATCTGAATATTTACAGAAAGCAGAAGTTTATATTGATGGGTTTTATAGCTTTA
CACCCCAGGAATTTATGATTGTAGAGCAGTTAATGAAGCACTGCAAGAAAGTAACAATCACTTTAACCCTTGATCAGCCA
TTTAAGAAGAATCCGCCTGATGAACTGCATCTTTTTAGAATGTCAGGTGAAACCTGCCAGACTATTTATGAAATCGCTGC
ACAACAAGGACTTCAAGTAGAAGAAGTAATCTTGAACGGCCAGAAAAGATGGGGAAAGCCATCCTTAAAGCACCTTGAAG
AAAAGTTCGAATCACGCCCAGCAGAAGGTTATAAAGGGGAGACTGCTGTTCATATCGGCCATGCAGCAAATCGCAGAGCA
GAAATTGAAGGCATTGCTAGAAAAATTAATCGGCTAGTGCGAACAAAAGGGCTTCGTTATCGTGATATTGCCATTTTATC
TAGAAATGGCCAGGATTATTTTGATATTATTGGAACGATCTTTCATGATTATCAAATACCTTATTTTATCGATCAAAAAA
GGACGATGCTGCATCATCCGCTTGTTGAATTAATACGGTCAACTCTTGAGGTAATCAACGGAAACTGGAGATATGAGCCG
ATTTTTAGAGCAGTAAAAACTGAATTGTTATTTCCGGCAAAATTAAATGCCCATCATTTAAGGGAACAGATGGACAGGCT
TGAGAACTATTGCTTGGCTTATGGCATTCAAGGCGATAAATGGACGAATAAGAAAAGATGGATTTATCGGAGAATTAGAG
GCTTAGAGTTTGAAAGCTTTGGGCAAACCGATGCGGAAAAGGAAATAGAGCAGGAGTTAAATGAACTAAGAGATATGATC
ACTGGTCCAATCCTTAGACTATCGCGCCGCCTAAAGAAAGCTCATTCGGGACGGCAGTATTGTGAAGCTGTCTATTTATA
CCTAGAAGAATTAGATGTTCCGGCTAAATTAGAGAGCTGGAAGCTTGAAGAAGATAATAAGGGAAATTTAGTGAAATCCC
GTGAACATGAGCAGTCTTGGAATGCAATTATGGAGCTGCTTGACCAATATGTAGAAATGCTCGGGGAAGAGGAAGTTACG
TTAAAACAATTTGCTTCCATTCTTGAAGCTGGTTTGGAATCTCTTCGTTTTACCCTTATCCCTCCAGCGTTGGATGGGGT
TATGGCTGCTGATTTGGACATGTCGCGTCTTTCTGACATTAAAGCCGCCTTTATCATCGGAATGAATGATGGTGTCTTTC
CAGCTAAATTTTCTGAGGAGGGACTGCTAGCCGACAGTGACCGGGAACAGCTCCTTGTTAACGGTTTAAAAATTGCACCA
GCCAGCAAAACACGGCTCCTGGATGAAGAATTTATTGCCTATAAAGCATTTGCTACGCCTTCTGATTGGCTATTCATCAG
CTATCCGCTTGCCAATGAGGAAGGAAAAGCGCTGCTTCCGTCTTCCTATATAAAAAGATTAACAGATTTATTGCCTGATT
GTGTACACCATGATTATCTATCAGACCCTGCAGAGCTGCCAGAGAATGAACAGCTGGAGTATGCAGTAAATCTGCACACC
TCCTTAGCTTATTTAACATCCCAGATGGGGCTAAAGAAAAGAAATTATCCGATTTATGATTTCTGGTGGGATATTTATAA
TTATTTCCTGCATCATGAACAATGGAGAGGACAAGCTCTTAAAGTTTTATCTAGTTTAAATTATGAAAATAAGACAAAGC
AATTAAAAGAAGAAATGAGCAAGGATTTATATGGAGATATCATTCAGGCGAGTGTTTCAAGAATGGAGCTATTCCACGGA
TGTCCGTTTTCACATTTTGCCCAGCATGGTTTAAAGCTGAAGGAACGTCAAGTATTCCGGCTTGAAGCTCCCGATATCGG
AGATTTATTTCATGCCGCTTTAAAATACATCGCTGAAACTGTGATGAGCAAAAAACTTTCCTGGACTGATTTAACAAGAA
AGCAATGTGAAATCCTCGCACAAGAGGCGGTCGAAATGCTTGCTCCTAAATTACAAAATGAAATCCTGCTCAGCTCCAAT
CGTCATTATTATATTAAAAGGAAGCTGGAGCAAATTATTAGCCGTGCCTCTTATGTATTAAGTGAGCATGCAAAGATGAG
CGGATTTTCTCCAATCGGACTAGAACTCGGCTTTGGACCAAAGGGGGAGCTGCCTCCGCTTACGTTCCCGCTAAAAAATG
GAGCGAAGATGGAGCTTATCGGCAGGATCGACCGTGTGGATAAAGCAGAGGATAAGGATGGAGTATTTTTAAGAGTTATA
GACTATAAATCGAGTTCAAAGGATATAAACGTGAATGAAGTCTACTATGGTCTAGCCTTGCAAATGTTAACTTATTTAGA
TATTATTGTCTCCCATTCACATGCACTGATCGGGACAGAAGCAAACCCAGCAGGGATTTTATATTTCCATGTCCACAACC
CAATCATCAGTACTTCAAAAATGCTCACAATAGATGATATTGAGCAGGAGATATTTAAAAAGTTTAAAATGAATGGCCTC
ATATTAGGGGAAGAAAATGTGATTAAGCTAATGGATCAGACATTAGATAACGGCGACTCGTCCATCATTTCTGCAGGCTT
TAAGAAGGATGGTACACTATCTAAGCGTTCCAAAATTGCAAGCAAGCAAGAATTTGAACAACTCCGTCAGTATGTCCGAA
AAATGTATACGAAGACAGGAAATCAAATTACTGAAGGAAAAGTGGACATTTCTCCTTATAAAATGAAGGATAAAACACCA
TGTACATTTTGTTCCTTTAAACCTGTATGCCAATTCGATGAGTCACTAGGAGCAAATGAATACCGCATGCTTGTTCCTCA
GCCGAAAGATGATGTGCTAGCTAGTATTCGAAAGGAGGTAGGGAAGGATGAAATCAACTATTCCAGCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

52.528

99.829

0.524