Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   RRV45_RS00545 Genome accession   NZ_CP135435
Coordinates   107861..109240 (+) Length   459 a.a.
NCBI ID   WP_315666846.1    Uniprot ID   A0AA96MWW6
Organism   Bacillus sp. DTU_2020_1000418_1_SI_GHA_SEK_038     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 102861..114240
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RRV45_RS00525 (RRV45_00525) - 103220..103630 (+) 411 WP_410489351.1 CtsR family transcriptional regulator -
  RRV45_RS00530 (RRV45_00530) - 103655..104203 (+) 549 WP_315666843.1 UvrB/UvrC motif-containing protein -
  RRV45_RS00535 (RRV45_00535) - 104206..105285 (+) 1080 WP_315666844.1 protein arginine kinase -
  RRV45_RS00540 (RRV45_00540) clpC 105290..107737 (+) 2448 WP_315666845.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  RRV45_RS00545 (RRV45_00545) radA 107861..109240 (+) 1380 WP_315666846.1 DNA repair protein RadA Machinery gene
  RRV45_RS00550 (RRV45_00550) disA 109244..110317 (+) 1074 WP_315666847.1 DNA integrity scanning diadenylate cyclase DisA -
  RRV45_RS00555 (RRV45_00555) - 110515..111597 (+) 1083 WP_315666848.1 PIN/TRAM domain-containing protein -
  RRV45_RS00560 (RRV45_00560) ispD 111644..112336 (+) 693 WP_315666849.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  RRV45_RS00565 (RRV45_00565) ispF 112365..112844 (+) 480 WP_315666850.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49733.21 Da        Isoelectric Point: 7.4337

>NTDB_id=886451 RRV45_RS00545 WP_315666846.1 107861..109240(+) (radA) [Bacillus sp. DTU_2020_1000418_1_SI_GHA_SEK_038]
MAKRKTKFMCQECGYESPKWMGKCPGCGVWNKMVEEVEKAAGMRRGAFAHSQGSAILSKATPITSIETVSEPRIYTDLNE
LNRVLGGGIVRGSLVLIGGDPGIGKSTLLLQVSSQLANKNHSVLYISGEESMRQTKLRADRLGVASEHLLVYSETSLDEI
SRTIESVNPSFVIVDSIQTIFHPEVTSAPGSVSQVRECTAELMRIGKTKGIAIFIVGHVTKEGSIAGPRLLEHMVDTVLY
FEGERHHTYRILRAVKNRFGSTNEMGIFEMKEFGLEEVENPSEIFLEERSQGAAGSTVVASMEGTRPVLVEIQALISPTS
FGNPRRMATGIDHNRVPLLMAVLEKRVGMLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDKPTRATDCIIGEVGL
TGEVRRVSRIEQRVQEAAKLGFERVILPANNLGGWSGPKGIELVGVSSVSEALKATLGG

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=886451 RRV45_RS00545 WP_315666846.1 107861..109240(+) (radA) [Bacillus sp. DTU_2020_1000418_1_SI_GHA_SEK_038]
ATGGCCAAAAGAAAAACAAAGTTTATGTGTCAGGAATGCGGATACGAATCTCCAAAGTGGATGGGAAAGTGCCCGGGCTG
CGGTGTTTGGAATAAGATGGTGGAGGAAGTGGAGAAAGCAGCTGGTATGAGGAGAGGAGCCTTCGCACACTCACAGGGCT
CGGCCATCCTTTCAAAAGCAACACCCATTACCTCTATAGAAACTGTAAGCGAGCCTCGAATTTACACAGACTTGAATGAA
TTAAATAGAGTTCTTGGTGGAGGAATTGTCAGGGGCTCGTTAGTGCTGATTGGCGGTGATCCAGGGATTGGAAAATCGAC
CTTGCTGCTCCAGGTCTCTTCTCAATTAGCTAATAAAAATCATTCTGTCTTATATATTTCCGGTGAGGAATCCATGAGGC
AGACGAAATTACGTGCGGACCGATTAGGGGTTGCTTCCGAACATTTACTCGTTTATTCGGAAACAAGTCTCGATGAAATT
AGCCGCACGATTGAAAGTGTCAATCCTAGCTTTGTCATTGTCGACTCCATCCAGACGATTTTCCACCCAGAAGTCACCTC
TGCTCCAGGCAGTGTCTCTCAAGTAAGAGAGTGTACGGCGGAGCTAATGAGAATTGGGAAGACGAAAGGTATTGCCATTT
TTATTGTCGGGCATGTTACAAAGGAAGGCTCGATTGCAGGACCAAGATTATTGGAGCATATGGTAGATACAGTTCTTTAC
TTTGAAGGTGAGCGTCATCATACATACCGAATTTTACGAGCGGTCAAGAATCGATTTGGCTCCACGAACGAGATGGGGAT
CTTCGAAATGAAAGAGTTTGGGTTAGAGGAAGTGGAAAATCCATCAGAAATTTTCCTAGAAGAACGATCCCAAGGGGCAG
CTGGTTCAACCGTAGTAGCTTCAATGGAGGGTACTCGTCCAGTCCTTGTAGAAATTCAGGCTCTTATTTCACCAACAAGC
TTTGGAAATCCCCGAAGAATGGCAACCGGAATTGATCATAATCGAGTCCCGCTTTTAATGGCAGTCCTAGAAAAGCGAGT
AGGCATGCTATTGCAGAACCAAGATGCTTATCTAAAGGTTGCTGGGGGAGTTAAGCTGGATGAGCCGGCTATCGACTTGG
CAGTTGCCGTCAGCATCGCTTCTAGCTTTCGTGACAAGCCGACAAGAGCTACTGACTGCATTATAGGTGAAGTGGGTCTT
ACAGGTGAGGTCAGAAGGGTATCAAGGATAGAGCAGCGTGTTCAGGAGGCAGCTAAGTTAGGGTTTGAACGTGTGATTTT
ACCTGCTAATAACCTAGGGGGCTGGTCTGGGCCGAAGGGGATTGAGCTTGTAGGTGTTAGTTCTGTCAGCGAAGCGTTAA
AAGCAACTTTAGGAGGTTAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(70-223)

Subunit ChlI of Mg-chelatase (ChlI)

(365-431)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

77.391

100

0.776
  radA Streptococcus mitis NCTC 12261

62.115

98.911

0.614
  radA Streptococcus pneumoniae Rx1

62.115

98.911

0.614
  radA Streptococcus pneumoniae D39

62.115

98.911

0.614
  radA Streptococcus pneumoniae R6

62.115

98.911

0.614
  radA Streptococcus pneumoniae TIGR4

62.115

98.911

0.614
  radA Streptococcus mitis SK321

61.894

98.911

0.612