Detailed information NTG0886433

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RRP28_RS13435
• Genome accession: NZ_CP135285
• Coordinates: 2987634..2988803 (+)
• Length: 389 a.a.
• NCBI ID: WP_017682837.1
• Uniprot ID: A0A0K8M896
• Organism: Pseudomonas syringae pv. actinidiae strain Yunnan2.4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2982634..2993803

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RRP28_RS13420 (RRP28_13420)	-	2984246..2984800 (+)	555	WP_017684828.1	hypothetical protein	-
RRP28_RS13425 (RRP28_13425)	ltrA	2985285..2986652 (-)	1368	WP_032685472.1	group II intron reverse transcriptase/maturase	-
RRP28_RS13430 (RRP28_13430)	-	2987204..2987479 (-)	276	WP_002553334.1	peptidylprolyl isomerase	-
RRP28_RS13435 (RRP28_13435)	pilU	2987634..2988803 (+)	1170	WP_017682837.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RRP28_RS13440 (RRP28_13440)	-	2989743..2991320 (-)	1578	WP_017699732.1	ABC transporter ATP-binding protein	-
RRP28_RS13445 (RRP28_13445)	-	2991322..2992344 (-)	1023	WP_017682835.1	ABC transporter permease	-
RRP28_RS13450 (RRP28_13450)	-	2992344..2993408 (-)	1065	WP_003383303.1	microcin C ABC transporter permease YejB	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42669.81 Da
• Isoelectric Point: 5.8676

>NTDB_id=886433 RRP28_RS13435 WP_017682837.1 2987634..2988803(+) (pilU) [Pseudomonas syringae pv. actinidiae strain Yunnan2.4]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETLKPGEVAVVAQGLMDEEQKLEFLRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVKTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSENGVTSLTTPPPAPTGSSTASTAEWGLVDDDEPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=886433 RRP28_RS13435 WP_017682837.1 2987634..2988803(+) (pilU) [Pseudomonas syringae pv. actinidiae strain Yunnan2.4]
ATGGATTTCCCGGCGTTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTTTATCTTTCTACCGGAGCACCGCCGTG
TGCCAAATTCAACGGCGTGCTCAAACCGCTGGGCAGCGAAACCCTCAAGCCCGGCGAAGTGGCGGTGGTTGCCCAGGGGC
TGATGGACGAGGAACAGAAGCTCGAGTTTCTCCGGGAACTGGAGATGAACCTCGCGGTGTCATTGGCCGGCATTGGTCGG
TTCCGGATCAATATCTTCATGCAGCGCAACGAAGTGTCCATTGTCGCGCGTAACATCAAGCTTGATATTCCGCGCTTTGA
GGACCTGTTCCTGCCGCCGGTATTGCTCGACGTGATCATGGAAAAACACGGTCTGGTGCTGTTTGTCGGCGCCACCGGCT
CGGGCAAATCGACCTCGCTGGCGGCACTGATCGATTACCGTAACCGCAATGCCAGCGGGCATATCATCACCATTGAAGAC
CCGGTCGAATTTATCCATCGGCACAAGAAATCCATCGTCAATCAGCGGGAGGTCGGCGTCGACACCCGCAGCTTTCGCGC
GGCACTGAAAAACACCTTGCGTCAGGCCCCGGATGTGATTCTGATCGGCGAGATTCGCGATCGCGAAACCATGGAGCACG
CGCTGGCATTCGCCGACACCGGCCACCTGGCGATTTCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGAATC
ATCAACTTCTTCCCGGAAGAGCGGCGTGCGCAGCTGTTGCACGACCTTGGCAACAACCTCAAGGCGTTCGTGTCTCAGCG
GCTGGTAAAGACGCCTGATGGAAAACGGCGCGCGGCGGTGGAGGTGATGATGGGCACGCCGACCATCCGCGATCTGATCC
AGCGTAATGAGCTGACTGAACTCAAGGGCATCATGGAAAAGTCCGGGAGCCTCGGGATGCAGACGTTCGATACCGCGCTG
TTCAACCTGGCCGTCGAAGGTGCGATCAGCGAGGAAGAAGCGTTGAAGAATGCCGACTCGCAAAACAACGTGCGCCTTCG
CCTCAAACTGCACAGCGAAAATGGAGTGACCAGCCTGACCACGCCGCCCCCGGCACCGACCGGCAGCAGCACAGCCAGCA
CCGCCGAGTGGGGCCTGGTCGACGATGACGAGCCGGGGCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-248)

3D structure

• AlphaFold DB: A0A0K8M896

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.072	99.486	0.558
pilU	Acinetobacter baylyi ADP1	51.831	91.26	0.473
pilU	Vibrio cholerae strain A1552	51.429	89.974	0.463
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.426	98.458	0.388
pilT	Pseudomonas aeruginosa PAK	41.399	88.175	0.365