Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   RP314_RS02825 Genome accession   NZ_CP135199
Coordinates   548329..549714 (+) Length   461 a.a.
NCBI ID   WP_077722929.1    Uniprot ID   -
Organism   Bacillus velezensis strain CMML21-47     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 543329..554714
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RP314_RS02805 (RP314_02805) - 544476..545123 (-) 648 WP_060657503.1 YigZ family protein -
  RP314_RS02810 (RP314_02810) degS 545346..546509 (+) 1164 WP_003151385.1 two-component sensor histidine kinase DegS Regulator
  RP314_RS02815 (RP314_02815) degU 546586..547275 (+) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  RP314_RS02820 (RP314_02820) - 547373..548221 (+) 849 WP_003151387.1 DegV family protein -
  RP314_RS02825 (RP314_02825) comFA 548329..549714 (+) 1386 WP_077722929.1 DEAD/DEAH box helicase Machinery gene
  RP314_RS02830 (RP314_02830) - 549772..550056 (+) 285 WP_017418816.1 late competence development ComFB family protein -
  RP314_RS02835 (RP314_02835) comFC 550062..550751 (+) 690 WP_202980098.1 phosphoribosyltransferase family protein Machinery gene
  RP314_RS02840 (RP314_02840) - 550825..551244 (+) 420 WP_043867469.1 TIGR03826 family flagellar region protein -
  RP314_RS02845 (RP314_02845) flgM 551314..551580 (+) 267 WP_003151393.1 flagellar biosynthesis anti-sigma factor FlgM -
  RP314_RS02850 (RP314_02850) - 551595..552077 (+) 483 WP_003151394.1 flagellar protein FlgN -
  RP314_RS02855 (RP314_02855) flgK 552093..553610 (+) 1518 WP_015387629.1 flagellar hook-associated protein FlgK -
  RP314_RS02860 (RP314_02860) flgL 553622..554539 (+) 918 WP_003151396.1 flagellar hook-associated protein FlgL -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52525.88 Da        Isoelectric Point: 10.0531

>NTDB_id=886181 RP314_RS02825 WP_077722929.1 548329..549714(+) (comFA) [Bacillus velezensis strain CMML21-47]
MSEGASEFSEVRDFFYGRHLLRSEIPFSDQRINRFIEKEYITAEPSIIRRKNRYVCQRCGQSDQACFAAFWAPSAKRQIT
YCRACVMMGRADELTSLYSWNQAPENSWEPVKLSWEGTLTDGQKQAAAALTDAIKERQELLVWAVCGSGKTEMLFPGIEF
ALNHGLRVCVATPRTDVVLELLPRLKKAFEKVDVSALYGGSEDKGRLTPLMISTAHQLMRYRDIFDVMIIDEVDAFPFSA
DETLRFAVDKARKKNSALVYVTATPSDTLKKNAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLQKGKLPRSVTDWV
RQKLQSQLPVFLFVPSVHVLKKTTDYFQKLNIRAEGVHAEDTFRKDKVKRFRDGLLDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKHFSGDVVMFHFGMTNGMKKAKKHIEHMNKLAQKSKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=886181 RP314_RS02825 WP_077722929.1 548329..549714(+) (comFA) [Bacillus velezensis strain CMML21-47]
ATGTCCGAAGGTGCTTCAGAATTTTCAGAGGTGCGGGATTTCTTTTACGGCCGGCATTTATTGAGATCTGAGATACCGTT
TTCAGATCAGCGCATCAATCGTTTTATAGAAAAAGAGTATATAACGGCCGAGCCGTCAATCATCCGCCGGAAAAACCGTT
ACGTCTGCCAACGATGCGGTCAGTCTGATCAAGCCTGCTTTGCGGCATTTTGGGCGCCCTCTGCCAAAAGGCAGATTACC
TATTGCCGTGCCTGTGTCATGATGGGAAGGGCTGATGAACTGACATCATTATATTCTTGGAATCAAGCCCCTGAAAACAG
CTGGGAGCCTGTAAAGCTTTCTTGGGAAGGAACACTGACAGATGGACAGAAGCAGGCCGCCGCCGCGCTTACAGATGCGA
TAAAAGAGAGACAAGAGCTGTTAGTATGGGCGGTTTGCGGATCGGGGAAAACAGAGATGCTGTTTCCTGGTATCGAGTTT
GCCCTGAATCACGGTTTGCGCGTCTGTGTCGCGACTCCGCGTACAGATGTCGTACTTGAACTGCTGCCGAGACTGAAAAA
AGCATTTGAGAAAGTCGACGTTTCTGCTTTGTACGGCGGCAGCGAGGACAAAGGCCGCTTGACTCCGCTGATGATTTCAA
CCGCTCATCAGCTGATGCGCTACCGAGACATTTTTGATGTCATGATAATAGATGAAGTAGATGCGTTTCCTTTTTCAGCC
GATGAGACATTAAGGTTCGCTGTTGATAAAGCGAGGAAGAAAAACAGCGCCCTTGTTTATGTAACGGCAACTCCTTCAGA
CACATTAAAAAAGAATGCTGAAGCCGGTTTGCTGAAAAGCGTTCGCATACCGGCCAGATATCATCGGAAACCTCTCCCGG
AGCCGCGTTTTTTGTGGTGCGGCAATTGGAAGAAGAAGCTTCAGAAAGGAAAACTGCCGCGCTCTGTAACGGATTGGGTC
CGCCAAAAACTTCAATCACAGCTGCCGGTTTTCTTATTCGTTCCGTCTGTACACGTATTGAAAAAGACAACGGATTATTT
TCAGAAATTAAACATACGGGCAGAAGGCGTACATGCGGAAGACACATTCAGAAAGGATAAAGTGAAACGGTTCAGAGACG
GCCTGCTTGACCTGCTGGTAACGACTACGATTTTAGAAAGAGGAGTTACCGTACCCAAAGTTCAGACATGTGTCCTCGGC
GCAGAAGCACCTATCTTTACTGAGAGCGCGCTCGTACAGATCGCGGGCAGAACGGGAAGGCATTATAAACATTTCAGCGG
TGATGTCGTCATGTTTCATTTCGGAATGACAAACGGAATGAAAAAAGCAAAAAAACATATAGAACATATGAACAAATTGG
CACAAAAAAGTAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(328-421)

Type III restriction enzyme, res subunit (ResIII)

(118-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

62.826

99.783

0.627