Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   RPB93_RS06060 Genome accession   NZ_CP135060
Coordinates   1177850..1178893 (+) Length   347 a.a.
NCBI ID   WP_001013167.1    Uniprot ID   -
Organism   Bacillus cereus strain B126_1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1172850..1183893
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RPB93_RS06025 (RPB93_06025) - 1173218..1173706 (+) 489 WP_000764265.1 hypothetical protein -
  RPB93_RS06030 (RPB93_06030) - 1173741..1174052 (-) 312 WP_001093237.1 hypothetical protein -
  RPB93_RS06035 (RPB93_06035) - 1174305..1174520 (+) 216 WP_001008327.1 DUF3912 family protein -
  RPB93_RS06040 (RPB93_06040) - 1174608..1175000 (+) 393 Protein_1173 hypothetical protein -
  RPB93_RS06045 (RPB93_06045) - 1175079..1176473 (-) 1395 WP_001094344.1 L-cystine transporter -
  RPB93_RS06050 (RPB93_06050) - 1176581..1176823 (-) 243 WP_000440716.1 DUF2626 domain-containing protein -
  RPB93_RS06055 (RPB93_06055) - 1176949..1177644 (-) 696 WP_313755367.1 helix-turn-helix domain-containing protein -
  RPB93_RS06060 (RPB93_06060) comGA 1177850..1178893 (+) 1044 WP_001013167.1 competence type IV pilus ATPase ComGA Machinery gene
  RPB93_RS06065 (RPB93_06065) comGB 1178880..1179917 (+) 1038 WP_016123380.1 competence type IV pilus assembly protein ComGB -
  RPB93_RS06070 (RPB93_06070) comGC 1179929..1180228 (+) 300 WP_001178684.1 competence type IV pilus major pilin ComGC -
  RPB93_RS06075 (RPB93_06075) comGD 1180225..1180680 (+) 456 WP_098272476.1 competence type IV pilus minor pilin ComGD -
  RPB93_RS06080 (RPB93_06080) - 1180673..1180975 (+) 303 WP_000829464.1 hypothetical protein -
  RPB93_RS06085 (RPB93_06085) comGF 1180945..1181415 (+) 471 WP_033692316.1 competence type IV pilus minor pilin ComGF -
  RPB93_RS06090 (RPB93_06090) comGG 1181412..1181783 (+) 372 WP_001231318.1 competence type IV pilus minor pilin ComGG -
  RPB93_RS06095 (RPB93_06095) - 1181958..1182608 (+) 651 WP_113303575.1 2OG-Fe(II) oxygenase -
  RPB93_RS06100 (RPB93_06100) aroK 1182724..1183221 (+) 498 WP_000836653.1 shikimate kinase AroK -
  RPB93_RS06105 (RPB93_06105) - 1183260..1183460 (+) 201 WP_000106083.1 YqzE family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39092.47 Da        Isoelectric Point: 8.3536

>NTDB_id=884618 RPB93_RS06060 WP_001013167.1 1177850..1178893(+) (comGA) [Bacillus cereus strain B126_1]
MNGIEIFANAILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTIQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFIDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEADGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=884618 RPB93_RS06060 WP_001013167.1 1177850..1178893(+) (comGA) [Bacillus cereus strain B126_1]
ATGAATGGGATTGAAATTTTTGCGAATGCAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTATAGGAAAAGATTTAATGACGATACAGTGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTCAAATTTTTAGCATCTATGGACATAGGCGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCCACACTTCCAACAGTATATCAAGAAAGTCTTGTTATTCGTCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTTGTGTTTACTGGACCGACTGGATCTGGGAAGACGACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACGCTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGTTTCATAGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAGGCTAGCATTTATGAGCTACTATATGGATATGAGTTAAAACAAGCGCTTAAAGAAGCTGATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCATCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.908

100

0.559
  pilB Haemophilus influenzae 86-028NP

39.752

92.795

0.369