Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RMV18_RS12465 Genome accession   NZ_CP134774
Coordinates   2831295..2832404 (+) Length   369 a.a.
NCBI ID   WP_311886691.1    Uniprot ID   -
Organism   Pseudomonas sp. VD-NE ext     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2826295..2837404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RMV18_RS12450 - 2827271..2828203 (+) 933 WP_311886689.1 sugar kinase -
  RMV18_RS12455 - 2828317..2828592 (-) 276 WP_007912322.1 peptidylprolyl isomerase -
  RMV18_RS12460 - 2828644..2831181 (-) 2538 WP_311886690.1 PAS domain S-box protein -
  RMV18_RS12465 pilU 2831295..2832404 (+) 1110 WP_311886691.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RMV18_RS12470 - 2832516..2834471 (-) 1956 WP_311886692.1 acetoacetate--CoA ligase -
  RMV18_RS12475 hbdH 2834630..2835403 (-) 774 WP_034154286.1 3-hydroxybutyrate dehydrogenase -
  RMV18_RS12480 - 2835411..2836802 (-) 1392 WP_311886693.1 GntP family permease -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40892.68 Da        Isoelectric Point: 6.5811

>NTDB_id=883528 RMV18_RS12465 WP_311886691.1 2831295..2832404(+) (pilU) [Pseudomonas sp. VD-NE ext]
MEIDALLSILSNKNGSDLFLSTGAPPSARVDGVLTALSEQPFKNGETAAIAASLMDAEQRREFDRDLEMNLAISRAGIGR
FRVNIFKQRNDVSIVIRNVKLDIPRFADLKLPAVLLDTVMLKQGLILFVGATDSGKSTSLAALIDHRNRHSTGHIITIED
PIEYIHRHQRSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVLSTLHAINANQALDRI
INMFPQERRPQLLQTLGNNLKACVSQRLVRTEGGQRRAAVEVLLGTPTISDLIRHNELGDLKAMMEKSGEAGMQTFDQSL
FELVEEGAINEEEALKYSDSANNLKLRIKLHRETVGKVSPSSSDWGLMD

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=883528 RMV18_RS12465 WP_311886691.1 2831295..2832404(+) (pilU) [Pseudomonas sp. VD-NE ext]
ATGGAAATCGATGCGCTGTTGTCTATTTTGTCCAATAAAAACGGTTCCGACCTGTTTCTCTCCACGGGGGCACCGCCAAG
TGCGCGCGTCGACGGCGTGCTTACAGCGTTGAGCGAACAGCCGTTCAAGAACGGTGAAACCGCCGCCATCGCTGCCTCCT
TGATGGACGCCGAGCAACGGCGGGAGTTCGACCGGGATCTGGAAATGAACCTGGCGATTTCGCGAGCGGGCATCGGGCGT
TTTCGTGTGAACATCTTCAAGCAGCGCAACGATGTATCGATCGTCATTCGCAACGTCAAACTCGACATCCCGCGTTTCGC
CGATCTGAAACTGCCGGCAGTACTGCTCGACACCGTTATGCTCAAGCAAGGGCTGATCCTCTTTGTCGGGGCCACCGACT
CCGGTAAATCGACGTCGCTGGCCGCACTGATCGACCACCGCAATCGCCATAGCACCGGGCATATCATCACTATCGAGGAC
CCGATCGAGTACATCCATCGCCACCAGCGCTCGATCATCAATCAGCGTGAAGTCGGGGTCGATACGCGCAGCTTCCACGC
TGCGTTGAAAAACACTCTGCGTCAGGCCCCGGATGTGGTGCTGATCGGCGAAATCCGTGACCGCGAAACCATGGAACATG
CCTTGGCGTTCGCCGATACCGGGCATCTGGTGCTCTCGACGTTGCACGCGATTAACGCCAATCAGGCGTTGGATCGAATC
ATCAATATGTTCCCGCAAGAGCGGCGGCCGCAATTGTTGCAGACGTTAGGCAACAACCTGAAAGCCTGCGTCTCGCAACG
TCTTGTCAGGACGGAGGGTGGGCAGAGAAGGGCGGCAGTGGAGGTGTTGTTGGGGACACCGACTATCAGTGATCTGATAC
GCCACAATGAATTGGGCGATCTCAAAGCGATGATGGAAAAGTCCGGGGAGGCGGGTATGCAGACGTTTGATCAGTCGTTG
TTCGAATTGGTCGAAGAGGGCGCGATCAATGAGGAGGAAGCGTTGAAATATTCCGATTCGGCCAATAATTTGAAGTTGAG
GATAAAGCTGCATCGAGAGACCGTCGGCAAGGTCAGCCCGTCAAGCAGTGATTGGGGGCTGATGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(57-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.587

97.019

0.539
  pilU Acinetobacter baylyi ADP1

52.958

96.206

0.509
  pilU Vibrio cholerae strain A1552

50.992

95.664

0.488
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.763

91.328

0.363
  pilT Acinetobacter nosocomialis M2

39.701

90.786

0.36
  pilT Acinetobacter baumannii D1279779

39.701

90.786

0.36
  pilT Acinetobacter baumannii strain A118

39.701

90.786

0.36