Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   RKV28_RS08815 Genome accession   NZ_CP134534
Coordinates   1771945..1772631 (-) Length   228 a.a.
NCBI ID   WP_001802022.1    Uniprot ID   A0A7U7EYP5
Organism   Staphylococcus aureus strain KNIH_5618     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1766945..1777631
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RKV28_RS08795 rpsT 1767859..1768110 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  RKV28_RS08800 holA 1768155..1769129 (-) 975 WP_001282563.1 DNA polymerase III subunit delta -
  RKV28_RS08805 comEC 1769186..1771387 (-) 2202 WP_001557362.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  RKV28_RS08810 - 1771392..1771853 (-) 462 WP_000439693.1 ComE operon protein 2 -
  RKV28_RS08815 comEA 1771945..1772631 (-) 687 WP_001802022.1 ComEA family DNA-binding protein Machinery gene
  RKV28_RS08820 - 1772671..1773387 (-) 717 WP_044416994.1 class I SAM-dependent methyltransferase -
  RKV28_RS08825 rsfS 1773390..1773743 (-) 354 WP_001088020.1 ribosome silencing factor -
  RKV28_RS08830 yqeK 1773744..1774328 (-) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  RKV28_RS08835 nadD 1774318..1774887 (-) 570 WP_000725167.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  RKV28_RS08840 yhbY 1774890..1775180 (-) 291 WP_000955235.1 ribosome assembly RNA-binding protein YhbY -
  RKV28_RS08845 aroE 1775184..1775990 (-) 807 WP_000666757.1 shikimate dehydrogenase -
  RKV28_RS08850 yqeH 1776004..1777104 (-) 1101 WP_001280137.1 ribosome biogenesis GTPase YqeH -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25900.65 Da        Isoelectric Point: 9.6751

>NTDB_id=880795 RKV28_RS08815 WP_001802022.1 1771945..1772631(-) (comEA) [Staphylococcus aureus strain KNIH_5618]
MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENNSLSKLEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLDDADVSRINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHVKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTI

Nucleotide


Download         Length: 687 bp        

>NTDB_id=880795 RKV28_RS08815 WP_001802022.1 1771945..1772631(-) (comEA) [Staphylococcus aureus strain KNIH_5618]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAGTGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAATAGTTTGTCCAAATTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAAGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGATGATGCAGATGTAAGTCGAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGTAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACCGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTAGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGATATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7U7EYP5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus N315

100

100

1
  comEA Staphylococcus aureus MW2

97.368

100

0.974