Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   RKV27_RS06030 Genome accession   NZ_CP134533
Coordinates   1156523..1157209 (+) Length   228 a.a.
NCBI ID   WP_001792568.1    Uniprot ID   A0A133PXY3
Organism   Staphylococcus aureus strain KNIH_5844     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1151523..1162209
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RKV27_RS05995 yqeH 1152050..1153150 (+) 1101 WP_001280138.1 ribosome biogenesis GTPase YqeH -
  RKV27_RS06000 aroE 1153164..1153970 (+) 807 WP_000666757.1 shikimate dehydrogenase -
  RKV27_RS06005 yhbY 1153974..1154264 (+) 291 WP_000955231.1 ribosome assembly RNA-binding protein YhbY -
  RKV27_RS06010 nadD 1154267..1154836 (+) 570 WP_000725162.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  RKV27_RS06015 yqeK 1154826..1155410 (+) 585 WP_001019323.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  RKV27_RS06020 rsfS 1155411..1155764 (+) 354 WP_001088022.1 ribosome silencing factor -
  RKV27_RS06025 - 1155767..1156483 (+) 717 WP_000084829.1 class I SAM-dependent methyltransferase -
  RKV27_RS06030 comEA 1156523..1157209 (+) 687 WP_001792568.1 ComEA family DNA-binding protein Machinery gene
  RKV27_RS06035 - 1157301..1157762 (+) 462 WP_000439693.1 ComE operon protein 2 -
  RKV27_RS06040 comEC 1157767..1159968 (+) 2202 WP_020978202.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  RKV27_RS06045 holA 1160025..1160999 (+) 975 WP_001282562.1 DNA polymerase III subunit delta -
  RKV27_RS06050 rpsT 1161044..1161295 (-) 252 WP_001274017.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25863.49 Da        Isoelectric Point: 9.3305

>NTDB_id=880738 RKV27_RS06030 WP_001792568.1 1156523..1157209(+) (comEA) [Staphylococcus aureus strain KNIH_5844]
MVLLYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

Nucleotide


Download         Length: 687 bp        

>NTDB_id=880738 RKV27_RS06030 WP_001792568.1 1156523..1157209(+) (comEA) [Staphylococcus aureus strain KNIH_5844]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTCTAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAAGTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAGGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGAGGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGAAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTTGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A133PXY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

98.238

99.561

0.978
  comEA Staphylococcus aureus N315

97.357

99.561

0.969