Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilJ   Type   Machinery gene
Locus tag   RKE53_RS00860 Genome accession   NZ_CP134413
Coordinates   180073..182727 (-) Length   884 a.a.
NCBI ID   WP_104396692.1    Uniprot ID   -
Organism   Microcystis aeruginosa NRERC-214     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 175073..187727
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RKE53_RS00850 (RKE53_00850) - 177000..178337 (-) 1338 WP_311131211.1 AAA-like domain-containing protein -
  RKE53_RS00855 (RKE53_00855) - 178785..179996 (-) 1212 WP_311131212.1 glycosyltransferase -
  RKE53_RS00860 (RKE53_00860) pilJ 180073..182727 (-) 2655 WP_104396692.1 methyl-accepting chemotaxis protein Machinery gene
  RKE53_RS00865 (RKE53_00865) - 182821..183351 (-) 531 WP_104396691.1 chemotaxis protein CheW -
  RKE53_RS00870 (RKE53_00870) pilH 183447..183812 (-) 366 WP_104396690.1 response regulator transcription factor Machinery gene
  RKE53_RS00875 (RKE53_00875) - 184199..184291 (-) 93 WP_228009813.1 DUF4388 domain-containing protein -
  RKE53_RS00880 (RKE53_00880) hmpF 184941..186581 (+) 1641 WP_311131213.1 pilus motility taxis protein HmpF -
  RKE53_RS00885 (RKE53_00885) - 187185..187409 (+) 225 WP_104396687.1 hypothetical protein -

Sequence

Protein


Download         Length: 884 a.a.        Molecular weight: 95883.76 Da        Isoelectric Point: 4.3563

>NTDB_id=879982 RKE53_RS00860 WP_104396692.1 180073..182727(-) (pilJ) [Microcystis aeruginosa NRERC-214]
MPSGKEYAKLYGKANTAYCQGNLEGAAVIVKEMISKYPDDANVILLQGHIYVGLQQYSLAEGSYEKVLELAKNSANFFDL
VDYARRGLDQIQQLRFEAENGDFMIAAAESIAYSEAAFDPSQGFSNSLSGGSQAAALERQQEVMDWDSGLFNDEDLGEPT
AGTVDSYGDDFGESEATAFSSLILAGSDSQGSHWSGDFADGEPPFPFLEAAEIDSLQEGWGGKFGSTGEDTFTADWRENP
LTGRDQELFYADTLEPGTFAIDPQPPHRQGAKTGLNQGRVTQKRYEGDEARDFRELKLNDEDLDGFSQLHLTDIAEEVGE
SELFTGSGEEALTAGFVPEPSARLNASSAVGEPSFHPSRLEIPDDTRDSHLGPVSERLLRPVVEVNPGKLAFFVNASLGK
KQLILAALAGITPVILIFAVSTTSWLFSLSAAKPGPNSKEKTEKVAPKPAPASLSPFSQPVLTEMLLTGLGAFALTWLGL
QLLISQIKRSLEDLQSQFDHISEGNFNAKATIYSEDEFGQLANSFNQMARVVATTTTQAQGRAAETERERKYLQRQVIRL
LDDVEGAARGDLTVKAEVSADVLGAVADAFNLTIQNLREIVRQVKKAAKQVNQASTNSESFARNQSSDALRMAEELAVTL
NSVQMMTDSIQRVAENAREAEEVARTSSITALKGGDSVERTVAGILQIRDTVSETARKVKRLAEASQEISKIVAVVSQIA
SRTNLLALNASIQAARAGDAGRGFAVVADEVRQLADRAAKSLKEIEQIVLQIQSETGSVMTAMEEGIQQVIDVTERSEQA
KKSLEDIIQVSNRIDTLVRSITADTVKQRENSLNVAQVMQSVELTAQETSQESQQVAGSLQKLVSISRELLSSVERFKID
SEDN

Nucleotide


Download         Length: 2655 bp        

>NTDB_id=879982 RKE53_RS00860 WP_104396692.1 180073..182727(-) (pilJ) [Microcystis aeruginosa NRERC-214]
ATGCCGTCAGGAAAAGAATATGCGAAACTCTATGGAAAGGCGAACACCGCCTATTGCCAGGGCAACCTTGAGGGTGCCGC
CGTCATTGTCAAAGAAATGATCAGCAAGTATCCCGATGATGCCAATGTCATCCTTTTACAGGGACATATTTATGTAGGAC
TACAACAATACAGCTTGGCCGAGGGAAGTTACGAAAAAGTGCTGGAATTGGCTAAAAATTCTGCCAATTTCTTTGATCTA
GTAGATTATGCTCGACGGGGTCTCGATCAGATTCAGCAATTGCGTTTTGAAGCCGAGAATGGAGATTTTATGATCGCTGC
TGCGGAAAGCATCGCCTACAGTGAGGCAGCTTTCGATCCCAGTCAAGGTTTCAGCAATTCTTTGTCCGGGGGGTCGCAAG
CGGCTGCTCTCGAGCGGCAACAGGAGGTAATGGACTGGGATAGTGGTCTTTTTAATGACGAGGATCTCGGAGAGCCTACG
GCGGGTACAGTTGATTCCTATGGCGATGATTTTGGGGAATCCGAGGCAACCGCCTTTAGTTCCCTTATCCTGGCTGGTTC
AGACTCTCAAGGTTCCCACTGGTCCGGGGATTTTGCGGATGGAGAACCCCCTTTCCCCTTTTTAGAAGCAGCGGAGATTG
ACTCGCTCCAGGAAGGGTGGGGCGGTAAGTTCGGGTCAACGGGAGAAGACACTTTTACGGCTGACTGGCGGGAAAATCCC
CTCACCGGGAGAGATCAGGAGTTATTCTATGCCGATACCCTAGAACCAGGCACTTTTGCCATTGATCCGCAACCGCCCCA
CCGACAAGGGGCGAAAACTGGCCTTAATCAGGGCAGAGTCACTCAAAAAAGATACGAGGGAGACGAAGCCAGAGATTTTA
GAGAACTAAAACTCAATGATGAGGATTTAGACGGTTTTTCCCAACTGCATCTGACGGATATAGCCGAAGAAGTGGGGGAA
TCGGAGCTATTTACTGGTTCGGGAGAGGAAGCTTTAACTGCTGGTTTTGTCCCCGAACCCAGTGCGCGCCTTAATGCCAG
TTCTGCTGTGGGAGAGCCTAGTTTTCATCCTTCTCGTCTAGAAATCCCCGATGACACAAGAGATAGTCATCTTGGTCCTG
TTTCCGAGCGCCTGCTCCGGCCAGTGGTGGAAGTCAATCCGGGCAAATTGGCCTTTTTTGTCAATGCTTCTTTGGGCAAA
AAACAACTAATCCTAGCGGCTTTAGCGGGTATTACCCCTGTAATCTTAATTTTTGCCGTTAGTACCACCTCTTGGCTATT
TTCTCTATCGGCTGCCAAACCGGGCCCGAATAGCAAGGAAAAAACCGAAAAAGTTGCCCCGAAACCAGCCCCAGCCAGCC
TTTCCCCTTTCAGTCAACCGGTACTAACCGAGATGTTATTAACTGGACTGGGAGCTTTTGCCCTAACTTGGCTAGGTTTA
CAGTTGCTAATTAGTCAGATTAAGCGCAGTCTCGAGGATTTGCAAAGCCAATTCGATCATATCTCGGAAGGCAATTTTAA
CGCTAAAGCGACGATCTACTCCGAAGATGAATTCGGTCAACTGGCCAATAGTTTCAATCAGATGGCCCGGGTAGTGGCAA
CTACCACCACCCAGGCCCAAGGCCGCGCCGCCGAAACGGAACGGGAACGGAAATATCTACAAAGGCAAGTGATTCGACTT
CTCGATGATGTGGAAGGAGCGGCTCGGGGGGATCTGACCGTGAAGGCGGAGGTAAGTGCCGATGTTTTAGGGGCGGTGGC
CGATGCTTTTAATTTGACTATCCAAAACCTGCGCGAAATTGTCCGACAGGTGAAAAAAGCGGCAAAACAGGTGAATCAAG
CCAGTACTAATAGTGAATCTTTCGCCCGCAATCAGTCTAGCGATGCCCTGCGGATGGCGGAGGAATTGGCCGTCACCCTT
AATTCGGTACAGATGATGACCGACTCAATTCAACGGGTGGCAGAAAACGCCAGAGAAGCCGAAGAAGTCGCCCGCACTTC
CTCGATTACCGCTCTAAAGGGGGGTGATTCCGTGGAAAGAACCGTGGCGGGAATTCTGCAAATCCGCGACACCGTATCAG
AAACGGCGCGAAAAGTCAAGCGTTTGGCGGAAGCTTCCCAGGAAATCTCGAAAATTGTCGCGGTAGTCTCTCAGATTGCC
TCGCGGACTAATTTATTGGCTCTTAATGCCTCGATTCAAGCGGCGCGAGCGGGAGATGCGGGCCGCGGTTTTGCGGTGGT
GGCCGATGAGGTGCGTCAGTTGGCCGATAGAGCTGCTAAATCTCTTAAGGAAATTGAACAGATCGTTTTACAGATTCAAT
CGGAAACGGGTTCGGTAATGACGGCAATGGAGGAGGGGATTCAACAGGTTATCGATGTCACCGAGCGCTCGGAACAGGCG
AAGAAGTCCCTAGAAGATATTATCCAAGTTTCTAACCGCATCGATACGCTGGTGCGATCGATTACCGCCGATACGGTCAA
ACAACGGGAAAATTCCCTCAATGTCGCCCAAGTTATGCAGTCGGTGGAGTTAACCGCTCAGGAAACTTCCCAAGAATCTC
AACAGGTGGCGGGTTCTTTGCAAAAATTGGTGAGTATCTCCCGGGAGCTGCTTTCTTCGGTAGAACGGTTTAAGATAGAC
TCGGAAGATAATTAA

Domains

Predicted by InterproScan.

Methyl-accepting chemotaxis protein (MCP) signalling domain (MCPsignal)

(662-842)

HAMP domain (HAMP)

(482-530)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilJ Synechocystis sp. PCC 6803

49.891

100

0.518