Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   RHJ02_RS04900 Genome accession   NZ_CP134178
Coordinates   1043085..1045109 (-) Length   674 a.a.
NCBI ID   WP_206201318.1    Uniprot ID   -
Organism   Thermosynechococcus sp. TG215     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1038085..1050109
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHJ02_RS04870 (RHJ02_04870) - 1038334..1039012 (-) 679 Protein_947 IS630 transposase-related protein -
  RHJ02_RS04875 (RHJ02_04875) - 1039562..1039915 (+) 354 WP_206201314.1 hypothetical protein -
  RHJ02_RS04880 (RHJ02_04880) - 1039912..1040184 (-) 273 WP_149817855.1 DUF3143 domain-containing protein -
  RHJ02_RS04885 (RHJ02_04885) - 1040181..1040654 (-) 474 WP_206201315.1 J domain-containing protein -
  RHJ02_RS04890 (RHJ02_04890) - 1040663..1041883 (-) 1221 WP_206201316.1 type II secretion system F family protein -
  RHJ02_RS04895 (RHJ02_04895) pilT 1041920..1043017 (-) 1098 WP_206201317.1 type IV pilus twitching motility protein PilT Machinery gene
  RHJ02_RS04900 (RHJ02_04900) pilB/pilB1 1043085..1045109 (-) 2025 WP_206201318.1 GspE/PulE family protein Machinery gene
  RHJ02_RS04905 (RHJ02_04905) - 1045371..1045583 (+) 213 WP_206201319.1 hypothetical protein -
  RHJ02_RS04910 (RHJ02_04910) - 1045584..1045781 (+) 198 WP_206201320.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  RHJ02_RS04915 (RHJ02_04915) - 1045829..1047304 (+) 1476 WP_310822156.1 right-handed parallel beta-helix repeat-containing protein -
  RHJ02_RS04920 (RHJ02_04920) - 1047301..1048263 (-) 963 WP_310800520.1 hypothetical protein -
  RHJ02_RS04925 (RHJ02_04925) - 1048354..1049310 (+) 957 WP_206201323.1 glycosyltransferase family 2 protein -
  RHJ02_RS04930 (RHJ02_04930) leuD 1049303..1049893 (-) 591 WP_206201324.1 3-isopropylmalate dehydratase small subunit -

Sequence

Protein


Download         Length: 674 a.a.        Molecular weight: 74401.80 Da        Isoelectric Point: 5.5500

>NTDB_id=879131 RHJ02_RS04900 WP_206201318.1 1043085..1045109(-) (pilB/pilB1) [Thermosynechococcus sp. TG215]
MVNTSSSSSRKALTVRGRAASPTERAIVASGYASIDQVREAMNTARKTGKSLVTVLQEITGTTMPPDVLRQYHKQQLFEL
KVIYGVDCLDPELNRFPTEQIEELINTIVPIDTCRTYQVIPIAKHLDADPPYLLVAMVDPDNLQAIDNLTRLLRAHNLTL
KRMVITLEDYQRLIDPILNKQVAETTASKSAPAAIGDINIEEDIEAMGGLEELEGEQEVDLAEALKGAEDAPIIALVNKI
LAKALTEGVSDIHIEPQEEYLRIRFRKDGVLHQAFDPLPKKIVPAVVSRFKILADLDIAERRAPQDGRIRKMFQGRRVDF
RVNTLPSRWGEKVVLRILDNSATQLGLDKLITDPESLAIVREMTKRPFGLILVTGPTGSGKTTTLYSALAECNSPGVNIS
TAEDPIEYTLPGLTQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPSAIAR
LSEMGIEPFMVSASLIGVVAQRLMRRVCSECRIPYTPTREELARFGLSASKDVNLTLYKANKLTPEQIQAAKATGQPICS
KCGGLGYKGRVGVYEIMRITERLQRLITEGAPTEQIKEAAVEEGMKTLLAYSLGLVKEGLTTLEEVERVTFTDTGLEAEL
KAKRKSSLTCRTCGAEAQPEWLECPYCMTPRFVD

Nucleotide


Download         Length: 2025 bp        

>NTDB_id=879131 RHJ02_RS04900 WP_206201318.1 1043085..1045109(-) (pilB/pilB1) [Thermosynechococcus sp. TG215]
ATGGTCAACACATCGTCATCTTCTTCTCGGAAAGCACTTACCGTGCGCGGCAGAGCCGCTAGCCCCACAGAACGAGCCAT
TGTTGCCTCCGGTTACGCCAGTATCGATCAGGTGCGGGAAGCCATGAACACCGCCCGCAAAACGGGCAAGTCCCTCGTAA
CCGTGTTGCAGGAGATTACGGGCACCACCATGCCCCCCGATGTGTTACGCCAGTACCACAAGCAACAGCTTTTTGAACTC
AAAGTGATCTACGGGGTTGATTGCCTCGATCCCGAACTCAATCGTTTCCCCACCGAGCAAATCGAGGAACTGATCAACAC
GATTGTACCGATTGACACCTGCCGCACCTACCAAGTCATCCCCATTGCCAAGCACCTCGACGCTGATCCCCCCTATCTGC
TCGTGGCGATGGTGGATCCAGATAACCTCCAAGCCATTGACAACCTCACCCGCTTACTCCGCGCCCACAACCTCACCCTC
AAGCGAATGGTGATCACCCTAGAGGATTACCAACGCCTCATTGACCCGATTCTCAATAAACAGGTAGCTGAAACCACCGC
TAGCAAAAGCGCCCCGGCTGCGATCGGTGACATCAACATCGAAGAAGACATCGAAGCCATGGGCGGCCTCGAAGAGCTGG
AAGGGGAGCAGGAGGTTGACCTTGCTGAGGCGCTGAAAGGTGCAGAAGATGCTCCGATTATTGCCCTTGTCAACAAAATT
TTGGCCAAAGCCCTCACCGAAGGCGTTTCCGACATTCACATCGAACCTCAAGAGGAATACCTGCGGATTCGTTTCCGCAA
AGATGGGGTGCTTCACCAAGCCTTTGATCCACTGCCGAAGAAAATCGTGCCAGCCGTCGTTTCCCGCTTCAAAATCTTGG
CTGACCTTGATATTGCTGAGCGGCGTGCCCCCCAAGATGGTCGTATCCGCAAAATGTTCCAAGGGCGACGGGTGGACTTC
CGAGTGAATACCCTCCCCAGCCGCTGGGGCGAAAAAGTCGTACTGCGGATTCTCGATAACTCCGCCACCCAACTCGGTCT
CGACAAGCTGATTACTGACCCTGAGAGCCTTGCTATTGTGCGGGAGATGACCAAGCGCCCCTTTGGCCTCATCCTCGTTA
CAGGTCCCACTGGTTCAGGGAAAACCACCACGCTTTACTCTGCGCTGGCTGAATGTAATAGTCCCGGTGTCAACATCAGT
ACAGCGGAAGACCCGATTGAATACACGCTCCCCGGCCTAACGCAGGTACAGGTTATTCGCGAAAAAGGCATGGACTTTGC
CTCAATTTTGCGAGCCTTTCTCCGCCAAGACCCCGATGTAATTCTGGTGGGCGAAACCCGTGACAAAGAAACCGCAAAAA
CCGCCATTGAAGCGGCTTTGACGGGGCACTTGGTCTTGACAACCCTGCACACTAACGATGCCCCCAGTGCAATTGCCCGC
CTCTCGGAAATGGGCATTGAACCCTTTATGGTTTCCGCCTCCCTCATTGGGGTTGTTGCCCAACGGCTGATGCGGCGGGT
ATGCAGTGAGTGTCGGATTCCCTATACACCCACCCGTGAGGAACTGGCGCGCTTTGGCTTGTCTGCCTCCAAGGATGTCA
ACCTCACCCTCTACAAAGCCAATAAACTGACACCCGAGCAAATTCAAGCGGCTAAGGCAACTGGCCAACCCATTTGCAGT
AAATGTGGTGGTTTGGGCTACAAAGGACGGGTCGGCGTCTACGAAATTATGCGGATCACCGAGCGACTGCAACGGCTGAT
TACCGAAGGTGCCCCCACCGAGCAAATCAAGGAGGCGGCCGTCGAAGAGGGCATGAAAACCCTGCTTGCCTATAGCTTGG
GGTTAGTCAAGGAAGGACTCACCACCCTCGAAGAAGTGGAGCGCGTCACCTTTACGGACACTGGACTAGAGGCAGAACTC
AAGGCCAAACGTAAGAGTTCTTTAACCTGCCGTACCTGCGGTGCCGAGGCGCAGCCCGAATGGTTAGAATGCCCCTATTG
CATGACACCCCGCTTTGTGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(239-506)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(74-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

64.939

97.329

0.632
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.776

94.51

0.366